全文获取类型
收费全文 | 1471篇 |
免费 | 79篇 |
国内免费 | 1篇 |
出版年
2023年 | 2篇 |
2022年 | 14篇 |
2021年 | 23篇 |
2020年 | 15篇 |
2019年 | 22篇 |
2018年 | 40篇 |
2017年 | 34篇 |
2016年 | 49篇 |
2015年 | 93篇 |
2014年 | 87篇 |
2013年 | 106篇 |
2012年 | 104篇 |
2011年 | 123篇 |
2010年 | 74篇 |
2009年 | 39篇 |
2008年 | 103篇 |
2007年 | 81篇 |
2006年 | 83篇 |
2005年 | 93篇 |
2004年 | 78篇 |
2003年 | 67篇 |
2002年 | 59篇 |
2001年 | 25篇 |
2000年 | 10篇 |
1999年 | 8篇 |
1998年 | 12篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1995年 | 11篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 7篇 |
1990年 | 6篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1985年 | 5篇 |
1984年 | 11篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1972年 | 2篇 |
1971年 | 3篇 |
1970年 | 2篇 |
1966年 | 6篇 |
1952年 | 1篇 |
排序方式: 共有1551条查询结果,搜索用时 0 毫秒
1.
2.
3.
4.
B Nowicki M Rhen V V?is?nen-Rhen A Pere T K Korhonen 《Journal of general microbiology》1985,131(5):1263-1266
Immunofluorescence staining with fimbria-specific antibodies was used to study the organization of fimbriate cells in colonies of Escherichia coli strain 3040. The strain has both type-1 and S fimbriae and shows fast phase variation between the fimbrial types. Colonies stained in sectors whose length and number per colony were dependent on the fimbrial phase of progeny cells. It is proposed that such sectors result from fimbrial phase variation. 相似文献
5.
Summary Amino acid starvation of Escherichia coli relA mutants may be used as a method for efficient plasmid DNA amplification. Here we demonstrate DNA degradation which occurs at elevated temperatures (42–43°C) after plasmid amplification in amino acid-starved bacteria. These results may explain the previously described low efficiency of plasmid DNA amplification at elevated temperatures. 相似文献
6.
Jacek Hennig Jocelyn Malamy Grzegorz Grynkiewicz Janusz Indulski Daniel F. Klessig 《The Plant journal : for cell and molecular biology》1993,4(4):593-600
Salicylic acid (SA) has been proposed to play a role in the induction of pathogenesis-related (PR) proteins and systemic acquired resistance (SAR) in tobacco. Since SA is rapidly converted to salicylic acid β-glucoside (SAG) in tobacco, we have attempted to assess the role of SAG in pathogenesis by application of chemically synthesized SAG to tobacco leaves. SAG was as active as SA in induction of PR-1 gene expression. This induction was preceded by a transient release of SA, which occurred in the extracellular spaces. The existence of a mechanism that releases SA from SAG suggests a possible role for SAG in SAR. 相似文献
7.
Markéta Ondračková Maria Yu Tkachenko Veronika Bartáková Anna Bryjová Michal Janáč Grzegorz Zięba Kacper Pyrzanowski Yuriy Kvach 《Journal of fish biology》2023,102(2):426-442
In Poland, distribution of non-native pumpkinseed Lepomis gibbosus (Centrarchidae) is strictly limited to the Oder river basin, where it was introduced in the early 20th century. Recently, several populations have been found in waterbodies adjacent to the Oder, particularly in its lower reaches. In this study, we compare the genetic relatedness of populations in the Oder basin with other European populations using nuclear (microsatellite) and mitochondrial (partial cytochrome c oxidase subunit I; cox1) markers. Microsatellite analysis indicated that four populations in the lower Oder form a separate cluster, while one in the middle Oder clustered with Danubian populations, from where probably having been introduced. Microsatellite data suggested that the lower Oder populations differ from other non-native European populations, making it impossible to estimate the source of introduction. Nevertheless, analysis of cox1 indicated that Oder pumpkinseeds belong to the same haplotype as the vast majority of European populations. Parasitological examination confirmed the presence of two North American species, the monogenean Onchocleidus dispar and trematode Posthodiplostomum centrarchi, in the lower Oder, both previously unknown in the region. Fifteen other parasite species were acquired, including glochidia of invasive Sinanodonta woodiana. In the middle Oder, parasite infection was more limited. Fish from the Gryfino Canal, considered one of the most invasive populations in Europe, showed the highest parasite abundance and diversity, and the highest somatic condition and growth rate due to warm water released from the Dolna Odra power plant. Our results highlight significant differences in somatic condition and parasite infection in long-established non-native pumpkinseed populations in the same river system, reflecting mainly environmental conditions. 相似文献
8.
Stephen Nowicki Peter Marler Alicia Maynard Susan Peters 《Ethology : formerly Zeitschrift fur Tierpsychologie》1992,90(3):225-235
Song sparrow (Melospiza melodia) songs are composed largely of pure-tonal sounds. This paper investigates the role that learning plays in the development of the tonal structure of song sparrow songs, as well as the role that tonal quality plays in determining the suitability of songs as models for learning. 20 birds were trained with both normal pure-tonal songs and modified songs that included harmonic overtones. The harmonic-modified songs were obtained from birds singing in a helium atmosphere, the result of which is to perturb vocal tract resonances and thus alter a song's tonal quality. Subjects learned equally well from normal and harmonic models. Birds that learned material from harmonic models reproduced some of this material with harmonic overtones, but the majority of notes learned from harmonic models were subsequently reproduced as pure-tonal copies. Thus, the tonal structure of songs does not influence young song sparrows in their selection of song models, but there is a strong tendency to reproduce songs in a pure-tonal fashion, even if learned from harmonic models. 相似文献
9.
An assessment of the annual mass balance of carbon,nitrogen, and phosphorus in Narragansett Bay 总被引:11,自引:3,他引:8
Narragansett Bay is a relatively well-mixed, high salinity coastal embayment and estuary complex in southern New England (USA). Much of the shoreline is urban and the watershed is densely developed. We have combined our data on C, N, and P inputs to this system, on C, N, and P accumulation in the sediments, and on denitrification with extensive work by others to develop approximate annual mass balances for these elements. The results show that primary production within the bay is the major source of organic carbon (4 times greater than other sources), that land drainage and upstream sewage and fertilizer are the major sources of N, and that landward flowing bottom water from offshore may be a major source of dissolved inorganic phosphorus. Most of the nutrients entering the bay arrive in dissolved inorganic form, though DON is a significant component of the N carried by the rivers. About 40% of the DIN in the rivers is in the form of ammonia. Sedimentation rates are low in most of Narragansett Bay, and it appears that less than 20% of the total annual input of each of these elements is retained within the system. A very small amount of C, N, and P is removed in fisheries landings, denitrification in the sediments removes perhaps 10–25% of the N input, and most of the carbon fixed in the system is respired within it. Stoichiometric calculations suggest that some 10–20% of the organic matter formed in the bay is exported to offshore and that Narragansett Bay is an autotrophic system. Most of the N and P that enters the bay is, however, exported to offshore waters in dissolved inorganic form. This assessment of the overall biogeochemical behavior of C, N, and P in the bay is consistent with more rigorously constrained mass balances obtained using large living models or mesocosms of the bay at the Marine Ecosystem Research Laboratory (MERL). 相似文献
10.