排序方式: 共有23条查询结果,搜索用时 31 毫秒
1.
Siegbahn Nils Mosbach Klaus Grodzki Karola Zocher Rainer Madry Norbert Kleinkauf Horst 《Biotechnology letters》1985,7(5):297-302
Summary The multienzyme enniatin synthetase was covalently immobilized to N-hydroxysuccinimide activated agarose. The stability of the immobilized enzyme at 25°C was enhanced compared to the soluble enzyme. Immobilization experiments also indicated that the enniatins are synthesized by a single molecule and thus do not require interactions of several enzyme molecules. 相似文献
2.
Norbert Madry Rainer Zocher Karola Grodzki Horst Kleinkauf 《Applied microbiology and biotechnology》1984,20(2):83-86
Summary The multifunctional enzyme enniatin synthetase was immobilized by adsorption to propyl agarose. The immobilized multienzyme retained 45% of the activity of the free enzyme; an operational half-life of about 15 h was estimated. Selective synthesis of several different enniatin homologues was achieved with propyl agarose-bound enniatin synthetase. In addition to enniatin A, B, and C formation, a selective synthesis of non-naturally occurring depsipeptides, containing norvaline, norleucine, or -aminobutyric acid as sole amino acid moieties, was observed. 相似文献
3.
4.
5.
6.
7.
8.
9.
Jan Pinowski Andrzej Haman Leszek Jerzak Barbara Pinowska Miłosława Barkowska Andrzej Grodzki Krzysztof Haman 《Journal of thermal biology》2006
Using an electronic apparatus simulating a bird roosting in a nest at night, we examined the insulating qualities of Eurasian Tree Sparrow (Passer montanus) nests built in nest boxes under winter conditions. Nests of different construction were compared with an empty box, and with roosting in open air. Energy savings in an empty box accounted for 18%, in boxes with incomplete nests for 23% and in boxes with complete nests up to 36%. The insulating value of nests mostly depended on their completeness and the proportion of feathers in the lining. 相似文献
10.
J. Holuša K. Lukášová R. Wegensteiner W. Grodzki M. Pernek J. Weiser 《Journal of Applied Entomology》2013,137(3):181-187
The objective of the current study was to identify pathogens of the large larch bark beetle, Ips cembrae, which is a secondary pest that has produced several local outbreaks across Europe in recent years. Beetles were collected from pheromone traps, trap trees and emergence traps (Larix decidua) during 2007 to 2011 at 10 study sites in central Europe. A total of 3379 mature and callow beetles were examined with a light microscope, and only two microsporidian pathogens [Chytridiopsis typographi and a diplokaryotic microsporidium (probably Nosema sp.)] and two gregarines (Gregarina typographi and Mattesia schwenkei) were found. Within the I. cembrae populations, the infection rate for C. typographi ranged from 2 to 58%. Nosema sp. occurred in only two beetles in 2007 (at two study sites). G. typographi was recorded only in Austria and Croatia and only in 1–2% of the beetles in those countries. Mattesia schwenkei was observed solely in Croatia in 0.6% of the beetles in that country. Only one fungal pathogen in the genus Fusarium was found and only in two mature beetles (0.7%) in 2010. The pathogen species found during our study of I. cembrae were very similar to the pathogens previously identified for Ips typographus. No species‐specific pathogen was detected. 相似文献