Chromatin matrix activity in hepatocyte nuclei after disturbance of the hepatic vagal innervation

The level of matrix activity of extranucleolar and nucleolar chromatin in hepatocytes of white noninbred rats has been studied in dynamics after disturbance of the vagal innervation. In order to estimate the labelling intensity of nuclear structures, the histoautoradiographic method is used. After bilateral subdiaphragmal vagotomy at early stages (1-2 weeks) the level of the nucleoplasm and nucleolus increases essentially, and by the 30th day a tendency to its normalization is noted. A conclusion is made that the peripheral nervous system influences the intensity of metabolic processes in the nucleus.     

Effect of radioprotective agents on the epithelium of small intestine mucosa in irradiated animals

In experiments on irradiated (10 Gy) mice a study was made of the morphological changes in the small intestine mucosa and in its functional status estimated by absorption of stain uranine. It was shown that the preventive administration of cystamine and S-(omega)-aminopropyl-beta-aminoethylthiophosphoric acid to the exposed mice increased the proliferation processes in the crypt and the cell movement per villus providing a favourable effect of the radioprotective agents on the absorption function.     

Cytochemical research on the matrix activity and status of the histone component of the neurocyte chromatin in the rat cerebellar cortex during postnatal differentiation

Evident differences in the ammoniacal silver staining pattern of histones were demonstrated for neurones of different layers of adult rat cerebellar cortex. These differences were formed during postnatal differentiation. It has been also shown for Purkinje and granular cells that time-course of age-dependent changes in histone staining are not coincident with that for template activity of these cells.     

Experience with the international standardization of the biological activity of the 3d Standard for streptomycin

The activity of the third International Standard of streptomycin was determined to be equal to 755 IU/mg with the use of accurately weighed amounts of streptomycin and 78037 IU with the use of streptomycin ampoules. The Standardization Center of the WHO decided to use the whole content of the ampoule of the Third International Standard of streptomycin, the activity of which is determined to be equal to 78500 IU per an ampoule.     

Effect of neurotensin on pancreatic function in man

The effect of neurotensin on submaximally-stimulated hepatobiliary and pancreatic secretion was studied in 6 healthy subjects. An intravenous infusion of neurotensin 1.4 ± 0.3 pmol/kg/min, designed to reproduce plasma neurotensin immunoreactivity levels within the physiological range, produced a significant increase in pancreatic bicarbonate output. Plasma concentrations of pancreatic polypeptide rose by 83 ± 16 pmol/l and were associated with a small reduction in trypsin, but no significant change in bilirubin outputs.     

Testing the sterility of injection preparations of penicillin series antibiotics]

The efficiency of 3 variants of the method for determination of microbial flora was compared on the injection preparation of potassium benzylpenicillin artificially infected with Staph. aureus 209P and the spores of Bac. subtilis ATCC 6633 in different doses and with different amounts of the preparation in the vials. The procedure of the preparation dissolution in the vial with the thioglycol medium containing penicillinase proved to be most effective. The microbe detection amounted to 100 per cent. The procedure was less labour- and time-consuming since addition of penicillinase to each vial with the thioglycol medium was excluded. The risk of the medium occasional infection with microbial flora during the assay was decreased.     

Dry erythrocytic diagnostic agent for the determination of antiglobulins]

Dry erythrocytic diagnostic agents were obtained under experimental conditions for determination of antiglobulins forming in the organism of man and animals under the effect of serum preparations from the blood of horses and homologoum immunoglobulins. A study was made of the sera of 100 patients with tick-borne encephalitis treated with heterologous and homologous immunoglobulins of directed action; in response to the administration of horse gamma-globulin antiglobulins (in titres below 1 : 10000) appeared in the serum; they circulated in the blood for long periods and inhibited the accumulation of hormonal antibodies to the causative agent; in the majority of cases a high level of antiglobulins to the foreign protein correlated with the presence of remote side-reactions of the serum sickness type. In patients treated with immunoglobulin of human origin antiglobulins were determined in low titres, disappeared from the blood in 15--20 days and did not hinder the accumulation of antihemmagglutinins to the tick-borne encephalitis virus.