全文获取类型
收费全文 | 1874篇 |
免费 | 139篇 |
出版年
2024年 | 4篇 |
2023年 | 13篇 |
2022年 | 32篇 |
2021年 | 68篇 |
2020年 | 47篇 |
2019年 | 69篇 |
2018年 | 81篇 |
2017年 | 78篇 |
2016年 | 101篇 |
2015年 | 111篇 |
2014年 | 123篇 |
2013年 | 144篇 |
2012年 | 144篇 |
2011年 | 133篇 |
2010年 | 90篇 |
2009年 | 69篇 |
2008年 | 107篇 |
2007年 | 96篇 |
2006年 | 78篇 |
2005年 | 57篇 |
2004年 | 50篇 |
2003年 | 42篇 |
2002年 | 41篇 |
2001年 | 32篇 |
2000年 | 19篇 |
1999年 | 23篇 |
1998年 | 6篇 |
1997年 | 11篇 |
1996年 | 12篇 |
1995年 | 8篇 |
1994年 | 3篇 |
1993年 | 5篇 |
1992年 | 11篇 |
1991年 | 12篇 |
1990年 | 7篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1982年 | 8篇 |
1980年 | 5篇 |
1978年 | 3篇 |
1974年 | 2篇 |
1970年 | 4篇 |
1968年 | 8篇 |
1967年 | 2篇 |
1965年 | 4篇 |
1964年 | 2篇 |
排序方式: 共有2013条查询结果,搜索用时 15 毫秒
1.
F F Dias M K Shaikh Y B Bhatt D C Modi V R Subramanyam 《The Journal of applied bacteriology》1986,60(4):271-275
Mutants of Bacillus amyloliquefaciens resistant to at least 10 micrograms/ml of tunicamycin were isolated and shown to be pleiotropic. The mutants were more resistant to streptomycin, chloramphenicol, kanamycin and neomycin than was the parent strain but less resistant to penicillin G and tetracycline. They were more autolytic, presumably due to an altered cell wall. The mutants produced reduced levels of amylase, penicillinase and both metal and serine protease besides having an enhanced sporulation frequency and being more motile. 相似文献
2.
D.A.M. da Silva M.S. Fernandes E.H. Endo A.C.P. Vital E.A. Britta M.E. Favero J.C. Castro P.T. Matumoto-Pintro B.P. Dias Filho C.V. Nakamura M. Machinski Junior J.M.G. Mikcha B.A. de Abreu Filho 《Letters in applied microbiology》2021,72(1):41-52
The use of rosemary essential oil (RO) and its combination with nisin (RO+N) in preventing the multiplication of Alicyclobacillus acidoterrestris in orange juice was evaluated. The minimum inhibitory and bactericidal concentrations (MIC and MBC) for RO were both 125 μg ml−1 while RO+N displayed a synergistic effect. The use of RO and RO+N at concentrations of 1, 4 and 8× MIC in orange juice for 96 h was evaluated in terms of their sporicidal effectiveness. With regard to the action against A. acidoterrestris spores, RO at 8× MIC was sporostatic, whereas RO+N at 1× MIC was sporicidal. Morphological changes in the structure of the micro-organism after treatment were also observed by microscopy. Furthermore, flow cytometric analysis showed that most cells were damaged or killed after treatment. In general, the antioxidant activity after addition of RO+N decreased with time. The results demonstrate that using the combination of RO and nisin can prevent the A. acidoterrestris growth in orange juice. 相似文献
3.
4.
M N Melo P Williams N M Rocha E H Babá W Mayrink M S Michalick C A da Costa M Dias P A Magalh?es 《Memórias do Instituto Oswaldo Cruz》1987,82(4):557-561
Attempts have been made to characterize two strains of Leishmania that became infective to golden hamsters only after they had been maintained for several years in a chemically defined culture medium. Observations were made on the growth rates of promastigotes in vitro, course of infection in hamsters, morphology of amastigotes, and electrophoretic mobility patterns of eight isoenzymes. Information was obtained about the buoyant densities of n-DNA and k-DNA, and one strain was tested against monoclonal antibodies. The identity of both strains remains obscure. 相似文献
5.
Calli from hypocotyl and root explants of Digitalis obscura L. showed regeneration of adventitious shoots, roots and embryos when transferred to Murashige & Skoog medium supplemented with cytokinins alone or in combination with auxins. Optimum shoot-bud formation was achieved in the presence of IAA and BA, while roots mainly appeared either in absence of growth regulators or with IAA and Kn. Embryo formation took place only in those combinations that included Kn. Embryo development was influenced by the type of auxin, and precocious germination occurred in media with NAA. Mechanically isolated cells from hypocotyl- and root-derived calli were plated in MS medium supplemented with several IAA and BA combinations. Single cells were able to proliferate forming callus within 20–30 days in culture. In order to induce organogenesis, calli were transferred to various regeneration media. Shoot-bud differentiation efficiency depended on both callus origin and medium initially used for cell culture, best results being obtained in calli grown from hypocotyl-derived cells cultured in the presence of casein hydrolysate. A further subculture to medium containing coconut milk and lower concentrations of NH4NO3 and sucrose promoted shoot development. Rooting was readily achieved upon transferring shoots onto half-strength MS medium. Plantlets were ultimately established in soil.Abbreviations BA
benzyladenine
- BM
basal medium
- CH
casein hydrolysate
- CM
coconut milk
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indoleacetic acid
- Kn
kinetin
- MS
Murashige & Skoog
- NAA
naphthaleneacetic acid 相似文献
6.
D C Modi Y B Bhatt M K Shaikh V R Subramanyam F F Dias 《The Journal of applied bacteriology》1986,61(4):315-318
Anilogel-E, a cross-linked starch, can be used with distinct advantages where native starch or soluble starch are conventionally used, e.g. in scoring for amylolytic organisms, as an ingredient of fermentation media, and in enhancing protoplast regeneration. It is particularly useful for the direct visualization of amylase producing micro-organisms on solid media, making prior replication of colonies unnecessary. 相似文献
7.
Sphaerotilus natans required calcium for the production of sheaths and probably requires calcium for growth as well, though at a lower concentration. Neither strontium nor barium substituted for calcium. S. natans grew attached to the culture vessels of the continuous-flow apparatus even when no sheaths were produced. Tentative evidence showed that the requirement for calcium is shared by the manganese-oxidizing species S. discophorus. 相似文献
8.
Growth-promoting Activity of Spent Sulfite Liquor for Sphaerotilus natans Growing in a Continuous-Flow Apparatus 下载免费PDF全文
The calcium in ammonia-base spent sulfite liquor, unlike that in calcium-base spent liquor, is not available for growth of Sphaerotilus natans. It has not been possible to obtain a mixed slime with Sphaerotilus as the dominant organism in a continuous-flow apparatus fed spent sulfite liquor. 相似文献
9.
Dias FF 《Applied microbiology》1967,15(6):1512-1513
10.
Utilization of Inorganic Nitrogen Compounds by Sphaerotilus natans Growing in a Continuous-Flow Apparatus 总被引:3,自引:3,他引:0 下载免费PDF全文
Sphaerotilus natans was grown, attached, in a continuous-flow apparatus with inorganic nitrogen compounds (NH4Cl, NaNO2, or NaNO3) as the only sources of nitrogen. The growth rate with NH4+-containing medium did not differ from that with media containing glutamate or Casitone as the nitrogen source. 相似文献