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1.
Nucleotide variation at the hypervariable esterase 6 isozyme locus of Drosophila simulans 总被引:2,自引:0,他引:2
Esterase 6 (Est-6/EST6) is polymorphic in both Drosophila melanogaster and
D. simulans for two common allozyme forms, as well as for several other
less common variants. Parallel latitudinal clines in the frequencies of the
common EST6-F and EST6-S allozymes in these species have previously been
interpreted in terms of a shared amino acid polymorphism that distinguishes
the two variants and is subject to selection. Here we compare the sequences
of four D. simulans Est-6 isolates and show that overall estimates of
nucleotide heterozygosity in both coding and 5' flanking regions are more
than threefold higher than those obtained previously for this gene in D.
melanogaster. Nevertheless, the ratio of replacement to exon silent-site
polymorphism in D. simulans is less than the ratio of replacement to silent
divergence between D. simulans and D. melanogaster, which could be the
result of increased efficiency of selection against replacement
polymorphisms in D. simulans or to divergent selection between the two
species. We also find that the amino acid polymorphisms separating EST6- F
and EST6-S in D. simulans are not the same as those that separate these
allozymes in D. melanogaster, implying that the shared clines do not
reflect shared molecular targets for selection. All comparisons within and
between the two species reveal a remarkable paucity of variation in a
stretch of nearly 400 bp immediately 5' of the gene, indicative of strong
selective constraint to retain essential aspects of Est-6 promoter
function.
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2.
Endothelial progenitor cells (EPC) participate in revascularization and angiogenesis. EPC can be cultured in vitro from mononuclear cells of peripheral blood, umbilical cord blood or bone marrow; they also can be transdifferentiated from mesenchymal stem cells (MSC). We isolated EPCs from Wharton's jelly (WJ) using two methods. The first method was by obtaining MSC from WJ and characterizing them by flow cytometry and their adipogenic and osteogenic differentiation, then applying endothelial growth differentiating media. The second method was by direct culture of cells derived from WJ into endothelial differentiating media. EPCs were characterized by morphology, Dil-LDL uptake/UEA-1 immunostaining and testing the expression of endothelial markers by flow cytometry and RT-PCR. We found that MSC derived from WJ differentiated into endothelial-like cells using simple culture conditions with endothelium induction agents in the medium. 相似文献
3.
J Daugherty TM Evans T Skillom LE Watson NP Money 《Fungal genetics and biology : FG & B》1998,24(3):354-363
Classical studies on spore release within the Saprolegniaceae (Oomycetes) led to the proposition that different mechanisms of sporangial emptying represent steps in an evolutionary transition series. We have reevaluated this idea in a phylogenetic framework using internal transcribed spacer sequences of four genera. These data were compared with the response to osmotic stress exhibited by each taxon. Saprolegnia emerges as the most basal genus, sister to Achlya, Thraustotheca, and Dictyuchus. Achlya and Thraustotheca are most closely related, while Dictyuchus appears to have evolved along a separate evolutionary lineage. The resulting phylogenetic framework is consistent with the idea that the mechanism of sporangial emptying exhibited by Saprolegnia represents the plesiomorphic condition from which the other mechanisms were derived independently. These alternative mechanisms of spore release may have resulted from a small number of mutations that inhibited axonemal development and altered the temporal and spatial expression of lytic enzymes that degrade the sporangial wall. Copyright 1998 Academic Press. 相似文献
4.
The genus Jorunna is characterized by a dorsum covered withcaryophyllidia, a prostate with two sections, a penis usuallyunarmed but occasionally armed with hooks, a copulatory spine,the presence of an accessory gland and a labial cuticle smoothor armed with jaw elements. The examination of 216 non-typespecimens, 30 types, and a review of the literature show thatthere are 16 valid species of the genus Jorunna: J. tomentosa(Cuvier, 1804); J. funebris (Kelaart, 1859); J. pantherinaAngas,1864; J. rubescens (Bergh, 1876); J. labialis (Eliot, 1908);J. parva (Baba, 1938); J. spazzola (Marcus, 1955); J. hartleyi(Burn, 1958); J. alisonaeMarcus, 1976; J. lemchei (Marcus, 1976);J. evansi (Eliot, 1906); J. pardusBehrens & Henderson, 1981;J.ramicolaMiller, 1996 and J. onubensis Cervera, García-Gómez& García, 1986. In addition, two new species fromthe Eastern Pacific are described: J. osae n. sp. and J. tempisquensisn. sp. We propose two new combinations: Jorunna parva and J.evansi. New records for the genus Jorunna are provided fromItaly, Algeria, Seychelles, Madagascar, Thailand, Marshall Islands,New Caledonia, Île de la Réunion, Sudan, PapuaNew Guinea, Indonesia, Panama, Costa Rica, Bahamas, and SouthernMexico. We present the first preliminary phylogenetic analysisof this cryptobranch dorid genus, based on morphological anatomicaldata, and discuss the biogeography and evolution of severalcharacters in this group. The phylogeny supports the hypothesisthat the genus Jorunna is a monophyletic group and shows thatKentrodoris is nested within it. (Received 31 December 2004; accepted 10 January 2008) 相似文献
5.
Systematics and biogeography of Pleurobranchus Cuvier, 1804 sea slugs (Heterobranchus: Nudipleura: Pleurobranchidae). Addendum
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6.
Irene TM Arkesteijn Lucas A Smolders Sandra Spillekom Frank M Riemers Esther Potier Bj?rn P Meij Keita Ito Marianna A Tryfonidou 《Arthritis research & therapy》2015,17(1)
IntroductionEarly degenerative changes in the nucleus pulposus (NP) are observed after the disappearance of notochordal cells (NCs). Thus, it has been suggested that NCs play an important role in maintaining the NP and may have a regenerative potential on other cells of the NP. As the number of resident NP cells (NPCs) decreases in a degenerating disc, mesenchymal stromal (stem) cells (MSCs) may be used for cell supplementation. In this study, using cells of one species, the regenerative potential of canine NCs was assessed in long-term three-dimensional coculture with canine NPCs or MSCs.MethodsCanine NCs and canine NPCs or MSCs were cocultured in alginate beads for 28 days under hypoxic and high-osmolarity conditions. Cell viability, cell morphology and DNA content, extracellular matrix production and expression of genes related to NC markers (Brachyury, KRT18) and NP matrix production (ACAN, COL2A1, COL1A1) were assessed after 1, 15 and 28 days of culture.ResultsNCs did not completely maintain their phenotype (morphology, matrix production, gene expression) during 28 days of culture. In cocultures of NPCs and NCs, both extracellular matrix content and anabolic gene expression remained unchanged compared with monoculture groups, whereas cocultures of MSCs and NCs showed increased glycosaminoglycan/DNA. However, the deposition of these proteoglycans was observed near the NCs and not the MSCs. Brachyury expression in the MSC and NC coculture group increased in time. The latter two findings indicate a trophic effect of MSCs on NCs rather than vice versa.ConclusionsNo regenerative potential of canine NCs on canine NPCs or MSCs was observed in this study. However, significant changes in NC phenotype in long-term culture may have resulted in a suboptimal regenerative potential of these NCs. In this respect, NC-conditioned medium may be better than coculture for future studies of the regenerative potential of NCs.
Electronic supplementary material
The online version of this article (doi:10.1186/s13075-015-0569-6) contains supplementary material, which is available to authorized users. 相似文献7.
A new species of zephyrinid nudibranch of the genus Janolus Bergh 1884 is described from the Pacific Coast of North America and Costa Rica. J. anulatus sp. nov. differs from other species of Janolus by its external and internal morphology. J. anulatus has a brown or white body with pink, white, and brown spots, smooth papillae epithelium at the base and papillated in the distal part, unbranched digestive gland ducts, smooth jaws, and smooth rachidian and lateral teeth. The species is compared with other species from the Panamic Province and the Western Atlantic. A new extension range of J. barbarensis is documented. 相似文献
8.
Margaret I Butler Jeremy Gray Timothy JD Goodwin Russell TM Poulter 《BMC evolutionary biology》2006,6(1):42-26
Background
We recently described a mini-intein in the PRP8 gene of a strain of the basidiomycete Cryptococcus neoformans, an important fungal pathogen of humans. This was the second described intein in the nuclear genome of any eukaryote; the first nuclear encoded intein was found in the VMA gene of several saccharomycete yeasts. The evolution of eukaryote inteins is not well understood. In this report we describe additional PRP8 inteins (bringing the total of these to over 20). We compare and contrast the phylogenetic distribution and evolutionary history of the PRP8 intein and the saccharomycete VMA intein, in order to derive a broader understanding of eukaryote intein evolution. It has been suggested that eukaryote inteins undergo horizontal transfer and the present analysis explores this proposal. 相似文献9.
10.
Species names and metaphyly: a case study in Discodorididae (Mollusca, Gastropoda, Euthyneura, Nudibranchia, Doridina) 总被引:1,自引:0,他引:1
Dayrat, B. & Gosliner, T. M. (2005). Species names and metaphyly: a case study in Discodorididae (Mollusca, Gastropoda, Euthyneura, Nudibranchia, Doridina) —Zoologica Scripta, **, ***–***. Absence of resolution in phylogenetic trees, or metaphyly, is a common phenomenon. It mainly results from the fact that each data set has its own limit and can hardly be expected to reconstruct alone an entire hierarchy. Because metaphyly helps point out which regions of a tree merit further investigation, one should not avoid metaphyly but rather should try to detect it by addressing carefully node reliability. In this paper we explore the implication of metaphyly for species names. We present a phylogenetic analysis of Discodorididae (Mollusca, Gastropoda, Nudibranchia, Doridina), with an emphasis on relationships among species of Discodoris and its traditionally close ‘allies’ such as Peltodoris and Anisodoris. We demonstrate that some species must be transferred to different discodoridid subclades with which they share synapomorphies, but that many species form a metaphyletic group at the base of Discodorididae, and therefore cannot be placed in any taxon of genus level. We demonstrate that the current International Code of Zoological Nomenclature does not allow taxonomists to handle this situation because it requires selecting a taxon name of genus rank for every species binomial. Then we evaluate the results provided by new forms of species names, both in a rank‐based system, such as the current nomenclature, and a rank‐free system. All solutions considered would cause radical changes to the ‘spirit’ of the current ICZN (and, by extension, to the other current codes). In a rank‐free system of nomenclature, such as the PhyloCode, the best result is obtained with an epithet‐based form that does not mention supra‐specific relationships. Under this method, official species names would take the form ‘boholiensis Bergh, 1877’, although page numbers and letters can be added for uniqueness purposes. Taxonomists would then be free to add supra‐specific taxon names in ‘common’ species names, such as Discodorididae boholiensis Bergh, 1877 or simply Discodorididae boholiensis. Here we wish to stimulate discussion of a problem that we believe merits wide debate: absence of resolution in phylogenetic reconstruction and its impact on species nomenclature. 相似文献