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I. S. Ivanov V. A. Lazarenko S. V. Ivanov G. N. Goryainova A. V. Ivanov 《Cell and Tissue Biology》2013,7(1):95-102
At present, the use of synthetic prostheses is an obligatory condition of surgical treatment of extensive and giant ventral hernias. We have carried out a comparative investigation of the dynamics of the collagen Type I to Type III ratio in the mouse paraprosthetic region upon use of the implants Esphyl (polyethylene), Ecophlon (polytetrafluorethylene), and Uniflex (polyvinylidenfluoride) with or without single and twofold introduction of cultured fibroblasts into paraprosthetic region at different time periods. Use of staining with Sirius Red and polarization microscopy revealed that, at a short time period (10 days after endoprosthesis implantation), the fibroblast introduction and the multiplicity of their introduction do not affect the collagen type ratio. At later time periods (30–60 days after the endoprosthesis implantation), the collagen type I ratio increased with use of all materials. At all steps of the experiment, the collagen type I/III ratio was the highest in the case of use of the Uniflex endoprosthesis. The exogenous fibroblasts accelerate the increase in the collagen type I/III ratio to a greater degree during twofold than upon single introduction. 相似文献
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A new efficient method for the parallel and sequential stepwise generation of single-domain antibodies to various high-abundance human-plasma proteins has been described. Single-domain antibodies have a number of features that favorably distinguish them from classical antibodies. In particular, they are able to recognize unusual unique conformational epitopes of native target proteins, small in size, and relatively easily produced and modified; have enhanced stability; and rapidly renature after denaturation. As a consequence, the immunosorbents that utilize these antibodies can be reused without any significant loss of activity. The principal novelty and universality of the described method is that it enables the sequential generation of antibodies to a number of high-abundance and yet unknown antigens of a complex protein mixture without the need for purified antigens. The effectiveness of the method is demonstrated by the example of generation of single-domain antibodies to a number of high-abundance proteins of the human blood plasma. The produced antibodies are promising biotechnological tools that can be used to develop prototypes for new diagnostic and therapeutic agents, as well as appropriate immunoaffinity-based methods for removal, enrichment, analysis, and/or targeting of specified proteins and their complexes from (in) the human blood. As we show, the generated single-domain antibodies can be efficiently used in designing new immunosorbents. As a rule, commercially available analogous immunosorbents that utilize classical antibodies remove many major proteins from the blood plasma immediately, while immunosorbents for many individual proteins are difficult to find and rather expensive. Single-domain antibodies generated by our method are unique new materials that allow for the development of more efficient and delicate approaches to pretreatment of plasma and the analysis of various blood plasma biomarkers. 相似文献
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S. V. Ivanov I. S. Ivanov G. N. Goryainova A. V. Tsukanov T. P. Katunina 《Cell and Tissue Biology》2012,6(3):309-315
To treat hernias, polymer net implants are widely used. We carried out a comparative study of the morphological picture of
inflammation, cellular composition of tissues, and dynamics of scar formation in mice in using the implants Esfil and Uniflex
under conditions of single and double introduction of cultivated fibroblasts and without fibroblasts in the area of disposition
of the endoprostheses. A more expressed inflammatory reaction throughout the entire period of time was observed in implantation
of the material Esfil. In using the material Uniflex, there was revealed a higher percent of fibroblasts than in using the
Esfil prosthesis. Introduction of cultivated fibroblasts in using both materials modified the curve of dynamics of the inflammatory
process by making it smoother. Use of the Uniflex endoprosthesis is preferable. 相似文献
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Tillib S. V. Goryainova O. S. Sachko A. M. Ivanova T. I. Gaas M. Ya. Vorob’ev N. V. Kaprin A. D. Shegay P. V. 《Molecular Biology》2022,56(4):616-627
Molecular Biology - A number of single-domain antibodies (nanobodies) obtained previously to major marker blood proteins were tested as tools to preprocess urine samples from patients with bladder... 相似文献
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