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1.
In this paper the recent population changes of the Wild Boar in different European countries is analysed through the study of hunting statistics. A simultaneous increase in numbers is observed throughout the whole area during the period 1965–1975. From 1975 onwards the population stabilizes itself apart from in peripheral areas like Finland. Potentially favourable factors which play a part in this process are discussed and certain reproductive and dispersive characteristics which favour its invasive behaviour are discussed. 相似文献
2.
V I Kiselev I M Tarasova A Iu Malinin O I Kiselev A I Glukhov 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1988,(9):21-26
A thermoresistant htpR mutant having a decreased level of proteolytic activity has been selected in E. coli strain K802 after the directed mutagenesis in vivo. The mutation results in the bacteriophage T7 RNA-polymerase stability, aminoglycosidephosphotransferase stability as well as in the decrease in the rate of proteolytic degradation of cytoplasmic proteins during the heat shock. The obtained mutant strain can, probably be used as a host for alien polypeptides production. 相似文献
3.
The chronic experiments on 24 male cats were carried out to study the influence of changes in functional interrelations between alpha- and beta-adrenoreactive amygdalar structures and corresponding receptors of vascular wall upon haemostatic system. It is shown that activation of alpha-adrenoreactive structures causes the hypercoagulative effect and that of beta-adrenoreactive receptors--the hypocoagulation activation. The central adrenoreactive structures realize their regulative influences through corresponding peripheral adrenoreceptors of the vascular wall. 相似文献
4.
To study the mechanism of precise excision ofgypsy from genomic sites, the integrase domain ofgypsy pol was cloned and expressed inEscherichia coli. The endonuclease activity of recombinant integrase was assayed with synthetic substrates corresponding to 3′-U5 ofgypsy LTR and to the known genomic insertion sites ofgypsy. Integrase nicked the 5′-A ⇓ YR-3′ triplet in the (+) strand of the double-stranded substrates; cleavage of a single-stranded
substrate was nonspecific. Cleavage proved to be affected by the local conformation of the substrate: the (+) strand was cleaved
more efficiently when the (−) strand had an unpaired base in the triplet and was not cleaved when the (−) strand was interrupted
or branched. The triplet corresponded to the consensus region ofgypsy insertion (5′-YRYR ⇓ YR-3′), the site of cleavagein vitro coinciding with the site of insertionin vivo. The unique mechanism ofgypsy excision was assumed to depend to a great extent on the enzymic properties of its integrase. 相似文献
5.
6.
Jing-Ming Yang Ya-Yue Liu Wen-Cong Yang Xiao-Xiang Ma Ying-Ying Nie Evgenia Glukhov 《Bioscience, biotechnology, and biochemistry》2020,84(8):1546-1553
ABSTRACT A new isoflavone derivative compound 1 (psoralenone) was isolated from soybean inoculated with a marine fungus Aspergillus terreus C23-3, together with seven known compounds including isoflavones 2–6, butyrolactone I (7) and blumenol A (8). Their structures were elucidated by MS, NMR, and ECD. Psoralenone displayed moderate in vitro anti-inflammatory activity in the LPS-induced RAW264.7 cell model. Compound 2 (genistein) showed moderate acetylcholinesterase (AChE) inhibitory activity whereas compounds 2, 5 (biochanin A), 6 (psoralenol), and 7 exhibited potent larvicidal activity against brine shrimp. Compounds 3 (daidzein), 4 (4?-hydroxy-6,7-dimethoxyisoflavone), and 5–7 showed broad-spectrum anti-microbial activity, and compound 7 also showed moderate 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. 相似文献
7.
I. A. Glukhov A. P. Kotnova Y. E. Stefanov Y. V. Ilyin 《Doklady. Biochemistry and biophysics》2016,466(1):1-4
A retrotransposon of the Mag family was found in the Drosophila simulans genome for the first time. We also identified novel transposable elements representing the Mag family in seven Drosophila species. The high similarity between the 3’ and 5’ long terminal repeats in the found copies of transposable elements indicates that their retrotransposition has occurred relatively recently. Thus, the Mag family of retrotransposons is quite common for the genus Drosophila. 相似文献
8.
9.
Effective selection and analysis of IS1 element transposition using the oLpLN region of phage lambda
The plasmid pNT6 permits selection of IS1-element insertions into the plasmid occurring with the frequency about 10%. The bacteriophage promoter pL cloned in pNT6 is a hot-spot region for IS1-element insertion. The frequency of IS1 transposition into pL depends on genotype. The plasmid pNT6 may be considered to be a useful target DNA for screening and analysis of IS1-element transposition. 相似文献
10.
I L Glukhov 《Molekuliarnaia biologiia》1990,24(5):1200-1210
A variety of approaches to determine the complete sequence of large DNA fragments is summarized. Main attention is paid to a transposon-based DNA sequencing strategy. 相似文献