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Polygalacturonase-inhibiting protein accumulates in Phaseolus vulgaris L. in response to wounding, elicitors and fungal infection 总被引:7,自引:0,他引:7
Carl W. Bergmann Yuki Ito Darrell Singer Peter Albersheim Alan G. Darvill Nicole Benhamou Laurence Nuss Giovanni Salvi Felice Cervone Giulia De Lorenzo 《The Plant journal : for cell and molecular biology》1994,5(5):625-634
Polygalacturonase-inhibiting protein (PGIP) is a cell wall-associated protein that specifically binds to and inhibits the activity of fungal endopolygalacturonases. The Phaseolus vulgaris gene encoding PGIP has been cloned and characterized. Using a fragment of the cloned pgip gene as a probe in Northern blot experiments, it is demonstrated that the pgip mRNA accumulates in suspension-cultured bean cells following addition of elicitor-active oligogalacturonides or fungal glucan to the medium. Rabbit polyclonal antibodies specific for PGIP were generated against a synthetic peptide designed from the N-terminal region of PGIP; the antigenicity of the peptide was enhanced by coupling to KLH. Using the antibodies and the cloned pgip gene fragment as probes in Western and Northern blot experiments, respectively, it is shown that the levels of PGIP and its mRNA are increased in P. vulgaris hypocotyls in response to wounding or treatment with salicylic acid. Using gold-labeled goat-anti-rabbit secondary antibodies in EM studies, it has also been demonstrated that, in bean hypocotyls infected with Colletotrichum lindemuthianum, the level of PGIP preferentially increases in those cells immediately surrounding the infection site. The data support the hypothesis that synthesis of PGIP constitutes an active defense mechanism of plants that is elicited by signal molecules known to induce plant defense genes. 相似文献
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Harshad S. Ugamraj Kevin Dang Laure-Hlne Ouisse Benjamin Buelow Eduardo N. Chini Giulia Castello James Allison Starlynn C Clarke Laura M. Davison Roland Buelow Rong Deng Suhasini Iyer Ute Schellenberger Sankar N. Manika Shipra Bijpuria Astrid Musnier Anne Poupon Maria Cristina Cuturi Wim van Schooten Pranjali Dalvi 《MABS-AUSTIN》2022,14(1)
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Human Cytomegalovirus UL131-128 Genes Are Indispensable for Virus Growth in Endothelial Cells and Virus Transfer to Leukocytes 下载免费PDF全文
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Vittorio de Franciscis Vittorio E. Avvedimento Giulia Colletta Vincenzo Zimarino Valeria M. Ursini Fortunato Ciliberto Giancarlo Vecchio 《Experimental cell research》1987,171(2):483-491
We have exploited a recently characterized system of rat thyroid epithelial cells transformed by the wild-type (wt) and a temperature-sensitive (ts) mutant strain of the Kirsten murine sarcoma virus (Ki-MSV) in order to study the effects of the K-ras oncogene on the gene expression of differentiated thyroid epithelial cells. By using cDNAs isolated from normal thyroid glands as probes, we were able to identify three sets of cellular sequences whose expression is influenced by the v-K-ras oncogene. The first set of genes is irreversibly repressed by transformation with both the wt and the ts viruses. The second set of genes is repressed in the ts-Ki-MSV-transformed cells but not in the same cells grown at the nonpermissive temperature. A third set of genes is present at higher levels at the nonpermissive temperature than at the permissive temperature. This system has allowed us to isolate and characterize a number of cDNA clones belonging to each of these three sets of genes. These specific cDNAs are suitable probes to study phenotypical changes during transformation of epithelial cells. 相似文献