全文获取类型
收费全文 | 238篇 |
免费 | 15篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 6篇 |
2015年 | 6篇 |
2014年 | 8篇 |
2013年 | 17篇 |
2012年 | 11篇 |
2011年 | 16篇 |
2010年 | 7篇 |
2009年 | 9篇 |
2008年 | 11篇 |
2007年 | 13篇 |
2006年 | 11篇 |
2005年 | 11篇 |
2004年 | 6篇 |
2003年 | 7篇 |
2002年 | 7篇 |
2001年 | 7篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 3篇 |
1992年 | 2篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 7篇 |
1987年 | 9篇 |
1986年 | 4篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1975年 | 5篇 |
1974年 | 1篇 |
1973年 | 4篇 |
1972年 | 1篇 |
1971年 | 4篇 |
1970年 | 1篇 |
1969年 | 3篇 |
1968年 | 3篇 |
1966年 | 1篇 |
1964年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有253条查询结果,搜索用时 15 毫秒
1.
V Busiello A Di Girolamo C Cini C Foppoli M Di Girolamo 《Physiological chemistry and physics and medical NMR》1987,19(1):23-27
The intracellular transport of thialysine and selenalysine in CHO cells has been studied. Data have been obtained indicating that the two lysine analogs can be transported by both the cationic aminoacid transport system and by the L transport system. The affinity of the cationic aminoacid transport system is similar for the two lysine analogs but lower than that for lysine and the affinity of the L transport system for the two lysine analogs is lower than that for leucine. 相似文献
2.
A new, simple and rapid procedure for the purification of high amounts of mitochondrial porins from different tissues of mammalia is described. The method consists in a single step hydroxyapatite / celite chromatography of Triton X-100 solubilized mitochondrial membranes. For optimal purification several factors are critical such as the absence of salts, a low protein / detergent ratio and an exact hydroxyapatite / celite ratio of 2:1. 相似文献
3.
4.
J. W. Grosser F. G. Gmitter Jr. N. Tusa G. Reforgiato Recupero P. Cucinotta 《Plant cell reports》1996,15(9):672-676
Summary Somatic hybridization experiments in Citrus that involve the fusion of protoplasts of one parent isolated from either nucellus-derived embryogenic callus or suspension cultures with leaf-derived protoplasts of a second parent, often result in the regeneration of diploid plants that phenotypically resemble the leaf parent. In this study, plants of this type regenerated following somatic fusions of the following three parental combinations were analyzed to determine their genetic origin (nuclear and organelle): (embryogenic parent listed first, leaf parent second) (1) calamondin (C. microcarpa Bunge) + Keen sour orange (C. aurantium L.), (2) Cleopatra mandarin (C. reticulata Blanco) + sour orange, and (3) Valencia sweet orange (C. sinensis (L.) Osbeck) + Femminello lemon (C. limon (L.) Burm. f.). Isozyme analyses of PGI, PGM, GOT, and IDH zymograms of putative cybrid plants, along with RFLP analyses using a nuclear genome-specific probe showed that these plants contained the nucleus of the leaf parent. RFLP analyses using mtDNA-specific probes showed that these plants contained the mitochondrial genome of the embryogenic callus donor, thereby confirming cybridization. RFLP analyses using cpDNA-specific probes revealed that the cybrid plants contained the chloroplast genome of either one or the other parent. These results support previous reports indicating that acquisition of the mitochondria of embryogenic protoplasts by leaf protoplasts is a prerequisite for recovering plants with the leaf parent phenotype via somatic embryogenesis following somatic fusion.Abbreviations cp
chloroplast
- GOT
glutamateoxaloacetate transaminase
- IDH
isocitrate dehydrogenase
- mt
mitochondria
- PEG
polyethylene glycol
- PGI
phosphoglucose isomerase
- PGM
phosphoglucomutase
- RFLP
restriction fragment length polymorphism
Florida Agricultural Experiment Station Journal Series No. R-04631. 相似文献
5.
Thiaisoleucine is an isoleucine analogue having the gamma-methylene group of the valerianic carbon chain substituted by a sulphur atom. It has been demonstrated that thiaisoleucine is activated and transferred to tRNAIle by rat liver aminoacyl-tRNA synthetase and inhibits isoleucine incorporation into polypeptides in protein synthesizing systems from rat liver or rabbit reticulocytes, whereas it does not affect either leucine incorporation or ribosome run-off or polypeptide chain elongation rate. All tests were performed in comparison with O-methyl-threonine, an isoleucine analogue with the gamma-methylene group substituted by an oxygen atom. In all the reactions studied, both thiaisoleucine and O-methyl-threonine act as competitive inhibitors of isoleucine. With respect to O-methyl-threonine, thiaisoleucine shows higher activity as an isoleucine inhibitor. 相似文献
6.
Accumulation of poliovirus Lsc-2ab by West Coast oysters was determined by using a stationary seawater system, and depuration was determined by using both stationary and free-flow systems. Results indicate that these shellfish have the same pattern of accumulation and localization of viruses as do East Coast species. However, uptake appeared to occur more rapidly than described for East Coast shellfish. There appeared to be a gradual diffusion of virus from the digestive area into the body. Depuration was found to occur more rapidly and completely under free-flow conditions than in a stationary system. 相似文献
7.
A variant clone of Chinese hamster ovary (CHO) cells resistant to thialysine has been isolated. It maintains the phenotypic properties even after 250 generations in medium without thialysine. Growth rate, cell viability and protein synthesis rate of the variant are much less affected by thialysine than the parental strain. In both the parental strain and the variant, thialysine acts in competition with lysine as indicated by the fact that all thialysine effects can be completely reversed by lysine. 相似文献
8.
Girolamo A. Vitello Francesco Calì Mirella Vinci Carmela Scuderi Francesca LEpiscopo Antonino Musumeci Sebastiano A. Musumeci Antonio G. Nicotera 《Journal of musculoskeletal & neuronal interactions》2020,20(4):610
Spinal muscular atrophy (SMA) refers to a group of genetic neuromuscular disorders affecting lower motor neurons causative of numerous phenotypes. To date, according to the age of onset, maximum muscular activity achieved, and life expectation four types of SMA are recognized, all caused by mutations in the SMN1 gene with SMN2 copy number influencing disease severity. Herein, we describe the case of a 31-year-old young male with normal psychomotor development who has experienced fatigue, cramps, and muscle fasciculations in the lower limbs for a period of 2 months. Based on electrophysiological and clinical findings we performed SMA genetic, clinical exome and RNA expression of candidate genes which led us to suggest SMN1-SMN2 genes [(2+0) and (0+0)] combination as possibly being implicated in the phenotype. 相似文献
9.
Vegetation History and Archaeobotany - Studies of the origins of agriculture in the Near East have revealed that the eight plant species known as “Founder Crops”, i.e. emmer, einkorn,... 相似文献