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A preliminary report on the use of transfer factor for treating stage D3 hormone-unresponsive metastatic prostate cancer 总被引:4,自引:0,他引:4
Dr. Giancarlo Pizza Caterina De Vinci Diego Cuzzocrea Domenico Menniti Ernesto Aiello Paolo Maver Giuseppe Corrado Piero Romagnoli Ennio Dragoni Giuseppe LoConte Umberto Riolo Aldopaolo Palareti Paolo Zucchelli Vittorio Fornarola Dimitri Viza 《Biotherapy》1996,9(1-3):123-132
As conventional treatments are unsuccessful, the survival rate of stage D3 prostate cancer patients is poor. Reports have
suggested the existence of humoral and cell-mediated immunity (CMI) against prostate cancer tumour-associated antigens (TAA).
These observations prompted us to treat stage D3 prostate cancer patients with an in vitro produced transfer factor (TF) able
to transfer, in vitro and in vivo, CMI against bladder and prostate TAA. Fifty patients entered this study and received one
intramuscular injection of 2–5 units of specific TF monthly. Follow-up, ranging from 1 to 9 years, showed that complete remission
was achieved in 2 patients, partial remission in 6, and no progression of metastatic disease in 14. The median survival was
126 weeks, higher than the survival rates reported in the literature for patients of the same stage. 相似文献
4.
Anna Rita Migliaccio Giovanni Migliaccio Giancarlo Mancini Mariusz Ratajczak Alan M. Gewirtz John W. Adamson 《Journal of cellular physiology》1993,157(1):158-163
The murine white (W) spotting locus is the site of the c-kit gene and encodes a tyrosine kinase receptor while the complementary Steel (Sl) iocus encodes its ligand. Mutations at either locus have profound effects on hematopoiesis, particularly erythroid and mast cell proliferation. We added c-kit antisense oligonucleotides to long-term suspension cultures of enriched human umbilical cord progenitor cells. This resulted in the suppression of c-kit gene expression and the preferential suppression of the generation of erythroid burst-forming cells (BFU-E) which extended over the life of the culture (3 weeks). The results provide an in vitro model of the “W phenotype” in human hematopoiesis and confirm the importance of c-kit gene function in early erythropoiesis. Because the generation of BFU-E was suppressed even after c-kit gene expression had recovered, this gene product may be critical to the erythroid commitment process. © 1993 Wiley-Liss, Inc. 相似文献
5.
M. Correale I. Abbate A. Paradiso F. Schittulli C. D. Dragone T. Tedone G. Gargano A. M. Catino M. D. Musci M. De Lena 《Cell biochemistry and biophysics》1993,22(1-3):101-110
Using a new immunoradiometric assay (ELSA pS2 Cis-France), a total of 200 cytosols obtained from primary breast tumors were examined for pS2 content, which is an estrogen-regulated protein actually studied as a marker of hormone sensitivity and favorable prognostic factor in breast cancer. In our patient group, the median pS2 value corresponding to 5.3 ng/mg of cytosolic proteins was used as cutoff. pS2 content was not related to menopause status, tumor size, or nodal involvement, whereas a positive correlation was found between pS2 and ER/PgR status. Moreover, the association of pS2 with steroid receptors seems to identify subgroups of patients better than ER/PgR alone. 相似文献
6.
Bruno Cavalleri Giancarlo Volpe Giogio Tuan Marisa Berti Francesco Parenti 《Current microbiology》1978,1(5):319-324
Two chlorophenyl-containing antibiotics have been isolated from a strain ofActinoplanes (ATCC 33002). Antibiotic A 15104 Y is a chlorinated phenylpyrrole compound whose structure has been confirmed by chemical
synthesis. Antibiotic A 15104 Z is a chlorophenol derivative for which a structural formula is proposed on the basis of its
physicochemical properties. A 15104 Y shows antimicrobial activity against Gram-positive, Gram-negative, and acid-fast bacteria,
yeasts, fungi, and protozoa, while A 15104 Z possesses a low activity against Gram-positive bacteria andTrichophyton. A 15104 Y has a weak activity in curing experimentally infected mice, at a dose that is one-fifth the LD50 for the same species. This is the first example of a chloropyrrole derivative isolated from an actinomycete. 相似文献
7.
Giancarlo Lunazzi Claudio Tiribelli Bruno Gazzin Gianluigi Sottocasa 《生物化学与生物物理学报:生物膜》1982,685(2):117-122
Bilitranslocase, a plasma membrane protein involved in bilirubin and other organic anion uptake by the liver, exhibits a high molecular weight (170 000) when isolated in the presence of deoxycholate. This value is decreased to approx. 100 000 if deoxycholate is not included in the isolation medium. Both preparations can be resolved into two kinds of subunit, α and β, of 37 000 and 35 500, respectively, by reduction with 2-mercaptoethanol and addition of sodium dodecyl sulfate. Under these conditions the two subunits are still capable of high-affinity sulfobromophthalein binding and, despite the presence of the detergent, may be isolated by preparative polyacrylamide gel electrophoresis still associated with the dye. It may be suggested that the physiological subunit composition of bilitranslocase is α2-β. 相似文献
8.
Arnold Demain Giancarlo Lancini 《Journal of industrial microbiology & biotechnology》1991,7(2):155-156
Society for Industrial Microbiology 相似文献
9.
Bruno Samorì Giorgio Lenaz Maurizio Battino Giancarlo Marconi Ida Domini 《The Journal of membrane biology》1992,128(3):193-203
Summary A general approach is developed to interpret linear dichroism (LD) spectra of ubiquinones (Q
n) in host bilayers. Information is reported in terms of guest-host mutual orientation and localization. The overall orientational anisotropy of guest ubiquinone molecules is described by a basic set of limiting orientation/localization modes. Assignments of the UV transitions of the ubiquinone chromophore were obtained by the liquid crystal-linear dichroism technique and molecular orbital (CNDO/S) calculations. The LD spectra of Q
n in the bilayers provided by the lyotropic nematic mesophase exhibited by water solutions of potassium laurate and decanol were interpreted on the basis of the above assignments. The resulting experimental evidence showed a multisite distribution in the host bilayer for the aromatic heads of all the investigated Q
n derivatives except Q0. The orientational distribution suggested by the LD spectra fits the solubilization model recently proposed by G. Lenaz [J. Membrane Biol. (1988) 104:193–209] for ubiquinone in lipid membranes. Within this model Q
n molecules are located in the midplane and their headgroups oscillate transversally across the membrane. Q
0 instead has a single site location, close to the polar bilayer interface. Experimental evidence that the headgroup carbonyls tend to grasp the polar interface of the host bilayer was also obtained. Orientation and location distributions of Q
n guest molecules are therefore likely to result from the tendency of their aromatic heads to grasp the polar heads of the host bilayer and from the concurrent tendency of their chains to settle into the hydrocarbon host interior.abbreviations AA
average absorption
- OD, OD
optical densities for plane polarized radiations parallel () and perpendicular () to the sample optical axis
- OD
OD — OD
- EPR
electron paramagnetic resonance
- LC-LD
liquid crystal-linear dichroism
- LD
linear dichroism
-
LD
r
reduced linear dichroism.
- MO
molecular orbital
- N
nematic
- NMR
nuclear magnetic resonance
-
S
jj
order parameters of the directions j of the transition moments of the guest chromophore
-
S
ii
order parameters of the orientational axes i of the guest molecule with respect to the magnetic field
-
S
ii
order parameters of the axes i of the guest molecules with respect to the bilayer axis a
-
S
a
order parameters of the host bilayer axis a with respect to the orienting magnetic field
-
j,i
deflection angles between the directions j and the axes i
-
O
i
optical factors of the i axis see Eq. (A4)]
- Qn
ubiquinone whose isoprenoid chain contains n isoprenoid units
Dr. A. Rossi is gratefully acknowledged for the t.e.m. reduction of the spectra. Ubiquinone homologs were kind gifts from Eisai Co., Tokyo, Japan. This work was supported by M.U.R.S.T., and C.N.R. Target Project on Biotechnology and Bioinstrumentation, Rome, Italy. 相似文献
10.
Giuseppe Bellucci Giancarlo Berti Maria Ferretti Franco Marioni Franca Re 《Biochemical and biophysical research communications》1981,102(3):838-844
The hydrolysis of (±)--3-bromo-1,2-epoxycyclohexane in the presence of rabbit liver microsomes was investigated, and found to yield, beside -3-bromocyclohexane--1,-2-diol, 2,3-epoxycyclohexanol. It was demonstrated that the latter compound was the only product of the enzymatic reaction, whereas the diol resulted from a non enzymatic hydration in the reaction medium. These data provide the first direct proof for a general base catalysis in the enzymatic epoxide hydration, previously hypothesized on the basis of several lines of indirect evidence, and disprove alternative mechanisms involving protonation of the oxirane oxygen. 相似文献