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1.
M. L. Getchell B. Zielinski J. A. DeSimone T. V. Getchell 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1987,160(2):155-168
Topical application of the odorants guaiacol (10(-3) mol/l, 1-30 min) and 2-isobutyl-3-methoxypyrazine (IBMP, 10(-5)-10(-3) mol/l, 15 min) caused time- and concentration-dependent reductions in the secretory granule content of acinar cells of the superficial Bowman's glands (sBG) and moderate to extensive vacuolation in acinar cells of sBG and deep olfactory glands (dG). Topical application of 9.8 mg/ml scopolamine 10 min before 10(-4) mol/l IBMP significantly reduced the amount of secretory granule depletion from sBG compared to that seen with IBMP alone and resulted in less extensive vacuolation in sBG and dG acinar cells. The i.p. injection of 42 mg/kg propranolol 10 min before topical application of 10(-4) mol/l IBMP had no effect on the action of IBMP. Guaiacol and IBMP also had time- and concentration-dependent effects on the secretory activity of sustentacular cells in the olfactory epithelium. The protrusion of secretory material into the mucociliary matrix that covers the epithelial surface and vacuolation within the secretory material resulted from odorant application. Scopolamine and propranolol had no effects on the action of IBMP on sustentacular cell secretory activity. When applied in the vapor phase, guaiacol elicited action potentials recorded from individual olfactory receptor neurons; the impulse frequency was concentration-dependent and showed tonic and phasic components when the duration of stimulation was varied. Low to moderate concentrations of IBMP delivered in the vapor phase evoked monophasic negative slow voltage transients recorded from the surface of the olfactory mucosa. The amplitudes of these transients increased with increasing stimulus concentrations. Higher concentrations or longer stimulus durations evoked longer-latency positive-voltage generating processes and negative afterpotentials. The properties of the electrophysiological responses to both odorants were characteristic of responses evoked by a wide variety of 'typical' odorants. 相似文献
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N. S. Rama Krishma Thomas V. Getchell Marilyn L. Getchell 《Cell and tissue research》1994,275(3):435-450
The expression of three classes of glutathione S-transferases (GSTs), Alpha, Mu, and Pi was investigated in the nasal mucosae of rats during development using immunohistochemical methods. GST Alpha and Mu were first detected in the supranuclear region of sustentacular cells on embryonic days 16. The Bowman's glands expressed differential patterns of immunoreactivity during development, beginning at postnatal day (P) 2 and P6 for Alpha and Mu classes, respectively and being greatest at P11 for both. The acinar cells of vomeronasal glands in the vomeronasal organ expressed Alpha and Mu classes of GSTs from P11 onwards. In the septal organ of Masera, the supranuclear region of sustentacular cells expressed GSTs from P11 with little or no variation during development. In the respiratory mucosa, Alpha and Mu classes of GSTs were detected at the brush borders of ciliated cells and in the acinar cells of posterior septal glands, but not in anterior septal or respiratory glands located on the turbinates. Compared to olfactory mucosa, the changes in immunoreactivity for GSTs were less pronounced in the respiratory mucosa during development. Specific GST Pi immunoreactivity was not detected in the nasal mucosae at any stage of development studied. The occurrence of GSTs in the nasal mucosa, including olfactory, vomeronasal, septal, and respiratory epithelia, suggests that the GSTs are actively involved in the biotransformation of xenobiotics including odorants and pheromones, and may also participate in perireceptor processes such as odorant clearance. In addition, we have developed a working model describing the cellular localization of certain phase I (e.g., cytochrome P-450s) and phase II (e.g., GSTs, -glutamyl transpeptidase) biotransformation enzymes in the olfactory mucosa and their proposed roles in xenobiotic metabolism. 相似文献
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The organization of the mucomicrovillar complex of the vomeronasal sensory epithelium of adult rats was examined using confocal laser scanning microscopy. In specimens labeled with the FITC-conjugated isolectin B4 of Bandeiraea simplicifolia, which recognizes terminal -galactose sugar residues of glycoconjugates, we demonstrated that the mucomicrovillar complex was composed of islet-like structures with a high-density -galactose core. The mucomicrovillar complex was further resolved into sensory and mucoid components in double-labeling and dual scanning experiments. The sensory component, which consists of the dendritic terminals of olfactory marker protein-immunoreactive vomeronasal receptor neurons, contained cytosolic glycoconjugates with terminal -galactose sugar residues. The extracellular mucoid component consisted of glycoconjugates containing terminal -galactose derived from the glands associated with the vomeronasal organ. These results demonstrated the complex microchemical organization of the sensory and mucoid components of the mucomicrovillar complex. 相似文献
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Detection of viral hemorrhagic septicemia in round gobies in New York State (USA) waters of Lake Ontario and the St. Lawrence River 总被引:1,自引:0,他引:1
Groocock GH Getchell RG Wooster GA Britt KL Batts WN Winton JR Casey RN Casey JW Bowser PR 《Diseases of aquatic organisms》2007,76(3):187-192
In May 2006 a large mortality of several thousand round gobies Neogobius melanostomus (Pallas, 1814) occurred in New York waters of the St. Lawrence River and Lake Ontario. Necropsies of sampled fish from these areas showed pallor of the liver and gills, and hemorrhagic areas in many organs. Histopathologic examination of affected tissues revealed areas of necrosis and hemorrhage. Inoculations of fathead minnow Pimephales promelas (Rafinesque, 1820) cell cultures with dilutions of tissue samples from the necropsied gobies produced a cytopathic effect within 5 d post-inoculation. Samples of cell culture supernatant were tested using RT-PCR and confirmed the presence of viral hemorrhagic septicemia virus (VHSV). Sequence analysis of the VHSV isolate resulted in its assignment to the type-IVb subgroup. The detection of VHSV in a relatively recent invasive fish species in the Great Lakes and the potential impact of VHSV on the ecology and economy of the area will require further investigation and careful management considerations. 相似文献
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A series of tests was conducted to characterize differences in the mortality of the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), and rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae), exposed to three commodities treated with a liquid and dry spinosad formulation. In laboratory bioassays, adults of the two insect species were exposed to untreated wheat, Triticum aestivum L., corn, Zea mays L., and sorghum, Sorghum bicolor (L.) Moench., and to commodities treated with 1 mg (AI)/kg of liquid and dry spinosad formulations. Mortality was assessed from independent samples examined at specific time intervals to determine immediate mortality and after 24 h of recovery on untreated grain at 28 degrees C and 65% RH to determine delayed mortality. Comparison of the time required for 50% (LT50) and 95% (LT95) mortality indicated that R. dominica adults were consistently and significantly more susceptible (died quickly) than S. oryzae adults when exposed to spinosad-treated commodities. In general, the toxicity of liquid and dry spinosad formulations was similar against R. dominica or S. oryzae. The toxicity of spinosad to each species varied slightly among the three commodities, and there were no consistent trends to suggest that spinosad was more effective on one commodity versus another. LT50 values based on immediate mortality for R. dominica on all commodities ranged from 0.45 to 0.74 d; corresponding values based on delayed mortality ranged from 0.04 to 0.23 d, suggesting delayed toxic action of spinosad in R. dominica. LT50 values based on immediate and delayed mortality for S. oryzae on all three commodities treated with the two spinosad formulations were essentially similar and ranged from 2.75 to 4.56 d. LT95 values for R. dominica based on immediate mortality on spinosad-treated commodities ranged from 1.75 to 3.36 d, and those based on delayed mortality ranged from 0.49 to 1.88 d. There were no significant differences in LT95 values based on immediate and delayed mortality for S. oryzae on spinosad-treated commodities, and the LT95 values ranged from 7.62 to 18.87 d. The toxicity of spinosad was enhanced during a 24-h holding period after removal from spinosad-treated commodities only against R. dominica adults, and possible reasons for increased postexposure mortality of R. dominica adults after brief exposures to spinosad warrant further study. 相似文献
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Colleen A. Burge Carolyn S. Friedman Rodman Getchell Marcia House Kevin D. Lafferty Laura D. Mydlarz Katherine C. Prager Kathryn P. Sutherland Tristan Renault Ikunari Kiryu Rebecca Vega-Thurber 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2016,371(1689)
Linking marine epizootics to a specific aetiology is notoriously difficult. Recent diagnostic successes show that marine disease diagnosis requires both modern, cutting-edge technology (e.g. metagenomics, quantitative real-time PCR) and more classic methods (e.g. transect surveys, histopathology and cell culture). Here, we discuss how this combination of traditional and modern approaches is necessary for rapid and accurate identification of marine diseases, and emphasize how sole reliance on any one technology or technique may lead disease investigations astray. We present diagnostic approaches at different scales, from the macro (environment, community, population and organismal scales) to the micro (tissue, organ, cell and genomic scales). We use disease case studies from a broad range of taxa to illustrate diagnostic successes from combining traditional and modern diagnostic methods. Finally, we recognize the need for increased capacity of centralized databases, networks, data repositories and contingency plans for diagnosis and management of marine disease. 相似文献
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Cells infiltrating the nonsensory epithelium of the vomeronasal organ of virus-antibody-free rats exhibited surface immunoreactivity for
2-microglobulin and immunoglobulin (Ig) E. They were further characterized by using immunohistochemical techniques with antibodies to cell-specific markers or histochemical techniques for immunocytes with surface receptors for IgE. Localization of intracellular granules immunoreactive for lactoferrin and CD18, a leukocyte adhesion molecule, unequivocally identified these cells as neutrophils. The low number of IgA-and IgG-immunoreactive B lymphocytes, T lymphocytes, and accessory immunocytes in the vomeronasal organ as well as the rest of the nasal cavity confirmed the absence of infection. We hypothesize that the operation of the vomeronasal pump induces repeated episodes of transient focal ischemia followed by reperfusion, which results in release of neutrophil chemoattractants and modulation of adhesion factors that regulate the extravasation and migration of neutrophils into the nonsensory epithelium. The distribution of immunoreactivity for interleukin 8 suggests that it is not the primary neutrophil chemoattractant in this system while that of CD18 suggests its active involvement in neutrophil extravasation. In addition to their role in immune surveillance, neutrophils may stimulate ion/water secretion into the vomeronasal lumen, affecting the perireceptor processes regulating stimulus access and clearance from the sensory epithelium. 相似文献
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The localization of -D-galactose, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine sugar residues of glycoconjugates in the vomeronasal organ, olfactory mucosa, and septal organ in the nasal mucosae of rats was investigated using lectinohistochemical techniques combined with bright-field, epifluorescence, and confocal laser scanning microscopy. Glycoconjugates in the mucomicrovillar complex of the vomeronasal organ contained all the sugar residues investigated, whereas glycoconjugates in the mucociliary complex of the olfactory mucosa and septal organ contained only N-acetyl-D-glucosamine. Vomeronasal receptor neurons expressed glycoconjugates with terminal -D-galactose and -N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine residues, whereas olfactory and septal receptor neurons expressed glycoconjugates with only N-acetyl-D-glucosamine residues. Secretory granules of glands of the vomeronasal organ contained glycoconjugates with terminal -D-galactose and N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine, whereas those of the Bowman's glands and glands of septal organ contained glycoconjugates with only internal N-acetyl-D-glucosamine residues. The results demonstrate that the glycoconjugates expressed by vomeronasal receptor neurons and glands contain terminal -D-galactose and -N-acetyl-D-galactosamine sugar residues that are not expressed by analogous cells in the olfactory mucosa and septal organ. 相似文献