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Plant Reactions to Inoculation of Roots with Fungi and Bacteria 总被引:1,自引:0,他引:1
The potential of 120 isolates of fungi and bacteria from plant rhizospheres to interfere with plant development and growth was studied in greenhouse experiments. The pure cultured isolates were obtained from plant roots in the field and applied as suspensions to the roots of eight test plant species. 10–20% of the isolates caused distinct symptoms on shoots, growth retardations without other symptoms or growth promotions. Responses of treated plants ranged from death of plants soon after treatment to up to about 40% higher shoot fresh weight than in control plants. Two bacterial isolates induced strong reactions in most of the plant species tested while other isolates showed a more or less pronounced specificity by giving reactions in only some of the plant species tested. 相似文献
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An isolate (G15) of a bacterium, frequently isolated from roots of various plant species, was identified asSerratia plymuthica. At low temperature (viz. 2–8°C), the studied isolated readily produced a red pigment which proved useful in recognizing the bacteria on reisolation.
In laboratory tests it exhibited strong antagonism againstBotrytis cinerea andGerlachia nivalis and moderate antagonism againstRhizoctonia solani, Fusarium culmorum andPythium sp. The bacterium significantly increased growth of lettuce plants when applied to the roots under non-sterile conditions
in greenhouse tests. Various strains ofSerratia plymuthica are supposed to be common as rhizosphere bacteria under Swedish conditions. 相似文献
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With the aim of elucidating mechanisms behind bacteria-induced deleterious effects and differential cultivar responses to bacterial inoculations, wheat seedlings were subjected to various tests under gnotobiotic conditions. Inoculation with two deleterious Pseudomonas isolates, Å 112 (fluorescent) and Å 313 (nonfluorescent), induced leaf symptoms and shoot and root growth inhibition, while inoculation with growthneutral bacteria (Serratia liquefaciens andEscherichia coli) had no such effects. Deleterious effects were induced at low inoculum densities (<103 cells per plant), but required addition of nutrient broth in small amounts for consistency. Effects similar to those obtained with living inoculum could be induced by treating plants with sterile culture filtrates from isolate Å 313 or volatile bacterial metabolites from isolate Å 112. Wheat cultivars previously found to differ in their reaction to inoculation under non-sterile conditions, responded differentially to Å 112 and Å 313 also in the gnotobiotic assay. The results agree with the hypothesis that neither cultivar reaction nor the bacterial effects as such are mediated by interactions with an indigenous rhizosphere microflora. 相似文献
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Characteristics of a plant deleterious rhizosphere pseudomonad and its inhibitory metabolite(s) 总被引:3,自引:0,他引:3
A cell-free culture filtrate of the plant growth inhibitory bacterial isolate Å313, identified as Pseudomonas fluorescens, was tested for its effect on wheat root elongation in vitro, with and without various pretreatments. The filtrate showed a strongly inhibitory effect on root elongation and could be heated to 100°C for 5 min or incubated at a pH within the range of 4–10 without losing its activity. Unlike other root growth-inhibitory bacterial metabolites the effect of the filtrate was not reversed by methionine, nor was any inhibitory activity present in the water phase after extraction with chloroform. The inhibitory agent(s) was formed when the bacterium was grown in an artificial medium as well as in root exudates from wheat. Two wheat cultivars differing in reaction to inoculation with living cells of Å313 showed the expected difference in growth reduction when exposed to culture filtrate, indicating a cultivar difference in sensitivity to the metabolite(s) formed by the bacterium. Isolate Å313 invaded intercellular spaces of the root cortex of gnotobiotically grown wheat plants, but did not produce pectolytic enzymes in vitro, nor induce a hypersensitive response in tobacco plants. 相似文献
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Selected rhizosphere bacterial isolates, previously determined as plant growth deleterious, were tested for their ability to suppress plant growth after foliar spray applications, for selectivity with regard to plant species, and in pilot field experiments for their potential as weed biocontrol agents. Inundative foliar applications of aqueous bacterial suspension were performed on a range of weed and crop species. Plant symptoms after spraying ranged from rapid necrosis and wilting to an overall growth suppression or stunting. Significant and selective reductions in biomass of up to 90% fresh weight, as well as large reductions in plant survival and plant height were recorded in greenhouse pot experiments. However, monocotyledonous plants were affected weakly or not at all by two isolates extensively tested. Effects of these were dose- and plant age-dependent, and were for some plants enhanced by high relative humidity. For one isolate, A153, effects were also expressed in cell-free culture filtrates pointing to involvement of specific metabolites. In pilot field experiments, strong growth suppression was observed on broad-leaved plants, while barley crop plants were unaffected. 相似文献
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In previous glasshouse experiments, the soilbacterium Serratia plymuthica, strainA153, showed strong growth-suppressingactivities against a range of broad-leavedweeds after foliar spraying. In field tests ofthis strain in spring wheat, spring barley andpotatoes, variable effects were achieved on arange of weeds including Chenopodiumalbum, Stellaria media, Polygonumconvolvulus and Galeopsis speciosa. Atone site, good suppression of C. albumwas observed when the strain was applied in atank mix with another bacterial isolate or withreduced doses of a herbicide. Effects on weedsappeared to be independent of the applicationvolume (1000, 600, 500 l ha–1), but weedswere in some cases more strongly suppressed athigher bacterial doses. Barley yields weresomewhat reduced by the bacterial application,but wheat yields were less affected. AlthoughS. plymuthica suppressed certain weedswhen applied in the field in a simple aqueousformulation and with conventional sprayingequipment, the level of weed suppression wasunsatisfactory from a practical standpoint. 相似文献
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Jens P. Levenfors Thomas H. Eberhard Jolanta J. Levenfors Berndt Gerhardson Margareta Hökeberg 《BioControl》2008,53(4):651-665
Seed lots of winter wheat and rye, naturally infested with Microdochium nivale and Fusarium spp., were treated with an isolate of Pseudomonas, which was recovered from roots of Brassica napus. Seeds were treated with bacterial fermentate and dried before sowing or they were directly sprayed in the furrow-opener
at the moment of sowing. Besides field experiments, parallel climate chamber bioassays were performed to assess the effect
of bacterial treatment on snow mould caused by seed-borne M. nivale and Fusarium spp. The biocontrol effect was assessed by plant density counts and by measuring yield. Significant biocontrol activity,
measured by plant density counts, was detected both in field and climate chamber experiments sown with wheat. Biocontrol effect
after spray application at sowing was less pronounced, although a slight increase in plant density was observed. The cell
concentration required to obtain adequate biocontrol effect was 109 CFU per ml for the dose used. The bacterial isolate was identified by 16S rDNA sequencing and biochemical tests as a Pseudomonas brassicacearum strain. 相似文献
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