Bioactive Components in Methyl tert-Butyl Ether Extract of Sea Buckthorn (Hippophae rhamnoides L.) Green Waste

Russian Journal of Bioorganic Chemistry - Lipophilic constituents of sea buckthorn leafy shoots, which is a large-scale waste product of sea buckthorn oil production and rejuvenating pruning of...     

Bioactive Components of Sea Buckthorn <Emphasis Type="Italic">Hippophae rhamnoides</Emphasis> L. Foliage

The composition of lipophilic components of sea buckthorn leafy shoots, a large tonnage waste in the production of sea buckthorn oil and during renewing the cultural plantings of sea buckthorn, was studied. Hexane was used as an extraction solvent for raw materials; it provides a high degree of lipophilic component extraction and is an analogue of extraction gasoline used in the food and perfume industries. The chemical composition of the hexane extract of sea buckthorn leafy shoots was studied by gas chromatography–mass spectrometry and high-performance liquid chromatography. Sixty-seven neutral and twenty-nine acidic components, including polyprenols, dolichols, triterpene alcohols and acids, sterols, were identified. β-Sitosterol was the main component of the sterol fraction. Its content was 6.9% of the extract mass, which is much higher than in the essential extracts of leaves and pulp of sea buckthorn fruit. It is mostly found in the free form in the extract. The acidic fraction contains highly active triterpene acids (up to 5% of the extract mass) along with the major aliphatic acids. Components with the chain length of 11 and 17 isoprene units predominate in the fraction of polyprenols and dolichols (up to 4.2%). The results allow us to consider sea buckthorn leafy shoots as a promising source of biologically active compounds.     

Composition and properties of wood and cellulose of tropical plants

A component, chemical and morphological composition of wood of the Howea fosteriana palm and the Cereus peruvians cactus and the wood stem of the Dracaena sanderiana was studied. The supramolecular structure and the physicochemical properties of cellulose samples which were extracted from the wood species were examined by wide-angle X-ray scattering, Fourier transform infrared spectroscopy, high-resolution ¹³CP/MAS nuclear magnetic resonance in a solid state, and scanning electron microscopy.     

Quantitative analysis of cellulose nitrates by Fourier transform infrared spectroscopy

Quantitative express analysis of nitrogen content in cellulose nitrates by Fourier transform infrared spectroscopy has been developed. The slope of the dependence of the ratio of the band intensity (and area) to sample weight in a tablet, on the nitrogen content in a sample was used to find the reduced extinction coefficients for quantitative analysis of nitrogen content in cellulose nitrate samples by IR spectroscopy. The results were compared with the nitrogen content values in the same samples determined by the ferrosulfate method.