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1.
The effects of ethylene oxide (EtO) on survival of soil microflora and on selected chemical properties of a sandy-loam soil
were examined.
Soil sterilization was achieved after 8 hours exposure to EtO. Ethylene oxide treatment increased soil pH and organic matter
content. Extractable Mn and Fe slightly increased whereas P content decreased. Total N was not affected by the treatment. 相似文献
2.
Pyridoxal phosphate-sensitized photoinactivation of glutamate decarboxylase from Clostridium perfringens 总被引:1,自引:0,他引:1 下载免费PDF全文
Ivo Cozzani Costantino Santoni Giulio Jori Giorgio Gennari Antonio Mario Tamburro 《The Biochemical journal》1974,141(2):463-468
1. l-Glutamate decarboxylase (EC 4.1.1.15) from Clostridium perfringens was inactivated by exposure to visible light at pH6.2. 2. Inactivation does not occur at pH4.6 or in the absence of bound pyridoxal phosphate. 3. On prolonged photo-oxidation six histidine residues per molecule of enzyme were destroyed. 4. The loss of six cysteine residues per molecule occurred both in irradiated samples and in controls oxygenated in the dark. 5. This dark-oxidation of cysteine residues is apparently required before the photo-oxidation process. 6. The absorbance, fluorescence and circular-dichroism properties of the enzyme as well as its elution volume during Sephadex gel-filtration were unaffected by prolonged irradiation. 7. However, an apparently homogeneous product of photo-oxidation could be separated from the control enzyme by ion-exchange chromatography. 8. The K(m) for l-glutamate was unchanged in an irradiated sample retaining 22% of control activity. 9. These data and the catalytic role of imidazole residues at the active sites of amino acid decarboxylases are discussed. 相似文献
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Osvaldo A. Sant'Anna Olga M. Ibanez Vera C. A. Ferreira Marisa Gennari Denise Mouton 《Immunogenetics》1983,18(4):359-364
High (H/s) and low (L/s) antibody responder lines of mice selected according to their response to the somatic (s) antigen of Salmonella (Selection IV) have unexpected inverse capacity for antibody production to rabbit gamma globulin (RGG): H/s mice are low or even nonresponders to this antigen, whereas L/s mice are high responders. It was shown that the phenotypic variability within each line is due to environmental factors. RGG was a selection antigen in Selection V; the high (H/p) and low (L/p) responder mice are therefore considered as homozygous for the RGG genes. Responsiveness to RGG was investigated in F1 and F2 hybrids obtained by crossing the phenotypically similar RGG responder or nonresponder mice of Selections IV and V. The results support the hypothesis that the same genes control the response to RGG in L/s and H/p lines as well as in H/s and L/p lines. This means that the genes specific for RGG responsiveness were independent from those regulating responses to the s antigen. Unaffected by the selective breeding in Selection IV, they have been fixed by chance in an inverse way in H/s and L/s lines. 相似文献
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