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2.
CHRISTER ERSÉUS 《Zoologica scripta》1990,19(1):57-63
A hypothesis of phylogenetic relationships within the family Tubificidae is presented, based on a Wagner analysis of morphological characters in the different subfamilies. Two major lincages are recognized. One, including the subfamilies Tubificinae, Telmatodrilinae and Limnodriloidinae, is supported by a synapomorphic ability to form slender spermatozeugmata in the spermathecae; the other, including Rhyacodrilinae (paraphyletic), Phallodrilinae, and the (present family) Naididae, is supported by two synapomorphics, the possession of modified penial setae and numerous coelomocytes (the latter secondarily lost in the Phallodrilinae). Some implications for the classification of the Tubificidae are discussed. 相似文献
3.
Foreign protein expression from the commonly used SV40 promoter has been found to be primarily during the S-phase of the cell cycle. Simple mathematical models with this cell cycle phase dependent expression of foreign protein suggest that the specific production rate will be proportional to the cell growth rate, which is particularly disadvantageous in high cell density fed-batch or perfusion bioreactors. In this study we investigate this predicted relationship between the production rate and growth rate by culturing recombinant CHO cells in a continuous suspension bioreactor. One CHO cell line, GS-26, has been stably transfected with the plasmid pSVgal, which contains the E. coli lac Z gene under the control of the SV40 promoter. This GS-26 cell line was grown in suspension cultures over a range of specific growth rates in batch and continuous modes. The intracellular -galactosidase activity was assayed using a standard spectrophotometric method after breaking the cells open and releasing the enzyme. A strong growth associated relationship is found between the intracellular -galactosidase content and the specific growth rate in batch and continuous cultures, as predicted. 相似文献
4.
The role of acid phosphatases in plant phosphorus metabolism 总被引:18,自引:0,他引:18
Hydrolysis of phosphate esters is a critical process in the energy metabolism and metabolic regulation of plant cells. This review summarizes the characteristics and putative roles of plant acid phosphatase (APase). Although immunologically closely related, plant APases display remarkable heterogeneity with regards to their kinetic and molecular properties, and subcellular location. The secreted APases of roots and cell cultures are relatively non-specific enzymes that appear to be important in the hydrolysis and mobilization of Pi from extracellular phosphomonoesters for plant nutrition. Intracellular APases are undoubtedly involved in the routine utilization of Pi reserves or other Pi -containing compounds. A special class of intracellular APase exists that demonstrate a clear-cut (but generally nonabsolute) substrate selectivity. These APases are hypothesized to have distinct metabolic functions and include: phytase, phosphoglycolate phosphatase, 3-phosphoglycerate phosphatase, phosphoenolpyruvate phosphatase, and phosphotyrosyl-protein phosphatase. APase expression is regulated by a variety of developmental and environmental factors. Pi starvation induces de novo synthesis of extra- and intracellular APases in cell cultures as well as in whole plants. Recommendations are made to achieve uniformity in the analyses of the different APase isoforms normally encountered within and between different plant tissues. 相似文献
5.
A simple neural network model is proposed for kindling — the phenomenon of generating epilepsy by means of repeated electrical stimulation. The model satisfies Dale's hypothesis, incorporates a Hebb-like learning rule and has low periodic activity in absence of shocks. Many of the experimental observations are reproduced and some new experiments are suggested. It is proposed that the main reason for kindling is the formation of a large number of excitatory synaptic connections due to learning. 相似文献
6.
Sharma Shivani Kumar Amit Dhakte Priyanka Raturi Gaurav Vishwakarma Gautam Barbadikar Kalyani M. Das B. K. Shivaraj S. M. Sonah Humira Deshmukh Rupesh 《Journal of Plant Growth Regulation》2023,42(1):46-59
Journal of Plant Growth Regulation - Crop improvement in light of the rapidly changing climate and the increasing human population continues to be one of the primary concerns for researchers across... 相似文献
7.
S. P. Gautam A. K. Gupta R. Shrivastava M. Awasthi 《World journal of microbiology & biotechnology》1996,12(1):99-100
Two thermostable enzymes produced by the thermophilic fungus Paecilomyces varioti, a chitinase and laminarinase, were used to isolate protoplasts of a thermophilic fungus, Malbranchea sulfurea. The frequency of protoplast regeneration observed (35%) was considerably higher than that obtained using commercial lytic enzymes. 相似文献
8.
CHRISTER ERSÉUS 《Zoologica scripta》1981,10(1):15-31
A taxonomic and morphological account of a collection of Phallodrilinae from coralline sands at Heron Island in Australia's Great Barrier Reef is given. Ten new forms are described: Jamiesoniella athecata gen. et sp.n. Phallodrilus rectisetosus heronensis subsp.n. P. geniculatus sp.n. P. filithecatus sp.n. P. clavatus sp.n. (latter three all devoid of an alimentary canal), Bathydrilus superiovasatus sp.n. Coralliodrilus atriobifidus sp.n. C. oviatriatus sp.n. C. parvigenitalis sp.n. and the gutless C. avisceralis sp.n. Morphological notes are given for Phallodrilus albidus Jamieson, 1977 and Bathydrilus rohdei (Jamieson, 1977). The new meiobenthic genus Jamiesoniella is characterized by having simple atria with only one pair of prostate glands, which are attached to ectal parts of atria, and by lacking spermathecae and penial setae, The genus is probably related to Aktedrilus Knöllner and Bacescuella Hrabě. A gutless species, Phallodrilus comorensis sp.n. is described from the Comoro Islands in the Mozambique Channel (W Indian Ocean). 相似文献
9.
Vema A Panigrahi SK Rambabu G Gopalakrishnan B Sarma JA Desiraju GR 《Bioorganic & medicinal chemistry》2003,11(21):4643-4653
Three-dimensional quantitative structure-activity relationship (3D-QSAR) models were developed for 100 anilinoquinazolines, inhibiting epidermal growth factor receptor (EGFR) kinase. The studies included molecular field analysis (MFA) and receptor surface analysis (RSA). The cross-validated r2 (r2cv) values are 0.81 and 0.79 for MFA and RSA, respectively. The predictive ability of these models was validated by 28 test set molecules. The results of the best QSAR model were further compared with structure-based investigations using docking studies with the crystal structure of EGFR kinase domain. The results helped to understand the nature of substituents at the 6- and 7-positions, thereby providing new guidelines for the design of novel inhibitors. 相似文献
10.
Dendrimers represents a highly branched three-dimensional structure that provides a high degree of surface functionality and versatility. PAMAM dendrimers are used as well-defined nanocontainers to conjugate, complex or encapsulate therapeutic drugs or imaging moieties. Star-burst [PAMAM] dendrimers represent a superior carrier platform for drug delivery. The present study was aimed at synthesis of a surface modified dendrimer for cancer targeted drug delivery system. For this 4.0 G PAMAM dendrimer was conjugated with Gallic acid [GA] and characterized through UV, IR, 1H NMR and mass spectroscopy. Cytotoxicity study of dendrimer conjugate was carried out against MCF-7 cell line using MTT assay. The study revealed that the conjugate is active against MCF-7 cell line and might act synergistically with anti-cancer drug and gallic acid-dendrimer conjugate might be a promising nano-platform for cancer targeting and cancer diagnosis. 相似文献