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排序方式: 共有149条查询结果,搜索用时 125 毫秒
1.
V Savelli R Rizzoli E Rizzi A Galanzi A Buffa R Rana G Lattanzi B Baratta 《Bollettino della Società italiana di biologia sperimentale》1991,67(12):1081-1088
In order to investigate the kinetics of vocal fold epithelium a bromodeoxyuridine-anti bromodeoxyuridine method has been applied in vivo at both light and electron microscopy level. This method is able to define the length of both epithelium turnover and cell-cycle in basal elements, as well as the existence of a higher proliferation rate during night time in comparison with day time. Moreover distinct labeling patterns observed in incorporating cells allow us to define the precise localization in S-phase of cycling elements. 相似文献
2.
O. Limaj M. Ortolani V. Giliberti A. Di Gaspare F. Mattioli S. Lupi 《Plasmonics (Norwell, Mass.)》2013,8(2):851-858
We studied surface plasmon sensors based on micrometric metal meshes by optical transmission spectroscopy as a function of the angle of incidence. The mesh period was set to 2 μm for operation at mid-infrared wavelengths. Metal meshes on dielectric substrates were compared to suspended meshes obtained with a lift-off-free fabrication process, which reduces plasmon damping and increases the quality factor up to 25. We have numerically calculated the electric field distribution of “dark” quadrupole-like modes and found that the suspended mesh provides an enhanced interaction volume extending up to hundreds of nanometers in free space. Our sensors have been experimentally tested and they exhibited a sensitivity up to 1.4?·?10?3?nm?1, at least 1 order of magnitude better than standard mid-infrared absorption spectroscopy. 相似文献
3.
Bonanomi Giuliano Zotti Maurizio Cesarano Gaspare Sarker Tushar C. Saulino Luigi Saracino Antonio Idbella Mohamed Agrelli Diana D’Ascoli Rosaria Rita Angelo Adamo Paola Allevato Emilia 《Plant and Soil》2021,460(1-2):263-280
Plant and Soil - Data about woody debris (WD) decomposition are very scarce for the Mediterranean basin. The specific aim of this work is to explore the relationships between WD traits with the... 相似文献
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Zannini C Turchetti S Guarch R Buffa D Pesce C 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》1999,21(4):358-362
OBJECTIVE: To collect quantitative and stereologic data on the main cell types represented in the seminiferous tubule in order to produce a three-dimensional representation of the morphologic events of normal cell production and maturation. STUDY DESIGN: Three-dimensional reconstruction and differential cell counting were performed on serial sections of normal testicular tissue from normal young men through image analysis and computer-based rendering. RESULTS: Peculiar periodicity in the total number of tubular cells considered along the sequence of serial sections was recognized. Also, consensual periodicity pertaining to each cell type was recognized. Adjacent high cellularity and low cellularity segments included a fairly constant mixture of cell types considered. CONCLUSION: A newly defined cellular wave, composed of regular alternations of high and low cellularity segments, was identified in the normal human seminiferous tubule. 相似文献
6.
Prognostic significance of standardized AgNOR analysis and Ki-67 immunostaining in gastrointestinal stromal tumors 总被引:2,自引:0,他引:2
Rodolico V Martorana A Gulotta G Diana G Leonardi V Cocorullo G Ajello F 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》2003,25(4):199-209
OBJECTIVE: To evaluate the prognostic utility of argyrophilic nucleolar organizer region (AgNOR) protein parameters and Ki-67-immunostained growth fraction (Ki-67 labelling index) and to correlate AgNORs with Ki-67 LI and the main clinicopathologic parameters in gastrointestinal stromal tumors (GISTs). STUDY DESIGN: On 55 patients with surgically excised GISTs, visualization and quantification of AgNORs were performed as specified in the guidelines of the Committee on AgNOR Quantification. RESULTS: AgNOR protein area (NORA) > or = 5.28 microns 2 was statistically associated with mitotic rate > or = 5 x 10 high-power fields (hpfs) (P < .001) and presence of necrosis (P < .001); Ki-67 LI > or = 9.69% was significantly associated with mitotic rate > or = 5 x 10 hpfs (P < .001), size > or = 5 cm (P = .033) and presence of necrosis (P < .001). Ki-67 LI and NORA strongly correlated. Preliminary Kaplan-Meier survival analysis showed that an increased value of NORA, Ki-67 LI, mitotic rate, tumor size and presence of necrosis had a negative influence on patient survival. Multivariate regression analysis showed that only NORA and Ki-67 LI were independent parameters in predicting the clinical outcome for patients with GISTs. Mitotic rate and necrosis remained as independent prognostic factors when NORA and Ki-67 LI were not allowed to enter in models. CONCLUSION: AgNOR protein quantity, as determined by image cytometry, and Ki-67 immunostaining seem to represent reliable predictive parameters in GISTs and are independent of mitotic rate, tumor dimension and necrosis. 相似文献
7.
Cossu G De Angelis L Borello U Berarducci B Buffa V Sonnino C Coletta M Vivarelli E Bouche M Lattanzi L Tosoni D Di Donna S Berghella L Salvatori G Murphy P Cusella-De Angelis MG Molinaro M 《The International journal of developmental biology》2000,44(6):699-706
In amniotes, myogenic commitment appears to be dependent upon signaling from neural tube and dorsal ectoderm, that can be replaced by members of the Wnt family and by Sonic hedgehog. Once committed, myoblasts undergo different fates, in that they can differentiate immediately to form the myotome, or later to give rise to primary and secondary muscle fibers. With fiber maturation, satellite cells are first detected; these cells contribute to fiber growth and regeneration during post-natal life. We will describe recent data, mainly from our laboratory, that suggest a different origin for some of the cells that are incorporated into the muscle fibers during late development. We propose the possibility that these myogenic cells are derived from the vasculature, are multi-potent and become committed to myogenesis by local signaling, when ingressing a differentiating muscle tissue. The implications for fetal and perinatal development of the whole mesoderm will also be discussed. 相似文献
8.
This study is an analysis of the patterns of variation of the human hand, particularly the metric characters of palm, fingers and distal phalanges. Anthropometric measurements were performed on 146 Sardinian men and women, aged 21 to 31 years. The data were analyzed by inferential statistics (paired Student's t test, analysis of variance), and Principal Components Analysis. The results indicate that size factors are the principal source of variation. A residual adimensional component of variability is related to diversification between the fingers as a whole and the distal phalanges, and between the thumb and the other fingers. Sexual dimorphism is evident. Men present greater dimensions and greater relative length of the thumb with respect to the other fingers than women. 相似文献
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