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Evangelos J. Giamarellos-Bourboulis Maria Raftogiannis Anastasia Antonopoulou Fotini Baziaka Pantelis Koutoukas Athina Savva Theodora Kanni Marianna Georgitsi Aikaterini Pistiki Thomas Tsaganos Nikolaos Pelekanos Sofia Athanassia Labrini Galani Efthymia Giannitsioti Dimitra Kavatha Flora Kontopidou Maria Mouktaroudi Garyfallia Poulakou Vissaria Sakka Periklis Panagopoulos Antonios Papadopoulos Kyriaki Kanellakopoulou Helen Giamarellou 《PloS one》2009,4(12)
Background
The pandemic by the novel H1N1 virus has created the need to study any probable effects of that infection in the immune system of the host.Methodology/Principal Findings
Blood was sampled within the first two days of the presentation of signs of infection from 10 healthy volunteers; from 18 cases of flu-like syndrome; and from 31 cases of infection by H1N1 confirmed by reverse RT-PCR. Absolute counts of subtypes of monocytes and of lymphocytes were determined after staining with monoclonal antibodies and analysis by flow cytometry. Peripheral blood mononuclear cells (PBMCs) were isolated from patients and stimulated with various bacterial stimuli. Concentrations of tumour necrosis factor-alpha, interleukin (IL)-1beta, IL-6, IL-18, interferon (FN)-alpha and of IFN-gamma were estimated in supernatants by an enzyme immunoassay. Infection by H1N1 was accompanied by an increase of monocytes. PBMCs of patients evoked strong cytokine production after stimulation with most of bacterial stimuli. Defective cytokine responses were shown in response to stimulation with phytohemagglutin and with heat-killed Streptococcus pneumoniae. Adaptive immune responses of H1N1-infected patients were characterized by decreases of CD4-lymphocytes and of B-lymphocytes and by increase of T-regulatory lymphocytes (Tregs).Conclusions/Significance
Infection by the H1N1 virus is accompanied by a characteristic impairment of the innate immune responses characterized by defective cytokine responses to S.pneumoniae. Alterations of the adaptive immune responses are predominated by increase of Tregs. These findings signify a predisposition for pneumococcal infections after infection by H1N1 influenza. 相似文献4.
Christianna Choulaki Garyfallia Papadaki Argyro Repa Eleni Kampouraki Konstantinos Kambas Konstantinos Ritis George Bertsias Dimitrios T. Boumpas Prodromos Sidiropoulos 《Arthritis research & therapy》2015,17(1)
IntroductionInterleukin-1β (IL-1β) is a major inflammatory cytokine, produced predominantly by innate immune cells through NLRP3-inflammasome activation. Both intrinsic and extrinsic danger signals may activate NLRP3. Genetic variations in NLRP3-inflammasome components have been reported to influence rheumatoid arthritis (RA) susceptibility and severity. We sought to assess the activity of NLRP3-inflammasome in patients with active RA compared to healthy individuals.MethodIntracellular protein expression of NLRP3, ASC, pro- and active caspase-1, pro- and active IL-1β was assessed by immunoblotting both at baseline and upon inflammasome activation. NLRP3 function (IL-1β secretion) was assessed upon priming of TLR2 (Pam(3)CysSK(4), TLR3 (poly(I:C)) or TLR4 (LPS) and ATP sequential treatment. We used caspase inhibitors (casp-1, 3/7 and 8) to assess their contribution to IL-1β maturation. All experiments were performed in whole blood cells.ResultsActive RA patients (n = 11) expressed higher basal intracellular levels of NLRP3 (p < 0.008), ASC (p < 0.003), active caspase-1 (p < 0.02) and pro-IL-1β (p < 0.001). Upon priming with TLR4 (LPS) and ATP, RA-derived cell extracts (n = 7) displayed increased expression of NLRP3 (p < 0.01) and active caspase-1 (p < 0.001). Secreted IL-1β in culture supernatants from whole blood cells activated with TLR4 (LPS) or TLR3 agonist (poly(I:C)) plus ATP was higher in RA patients (n = 20) versus controls (n = 18) (p < 0.02 for both). Caspase-1 inhibition significantly reduced IL-1β secretion induced by all stimuli, whereas caspase-8 inhibition affected only TLR4 and TLR3 cell priming.ConclusionPatients with active RA have increased expression of NLRP3 and NLRP3-mediated IL-1β secretion in whole blood cells upon stimulation via TLR3 and TLR4 but not TLR2. In these patients, IL-1β secretion seems to be predominately driven by caspase-1 and caspase-8. Targeting NLRP3 or downstream caspases may be of benefit in suppressing IL-1β production in RA. 相似文献
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Iga K. Mieczkowska Garyfallia Pantelaiou-Prokaki Evangelos Prokakis Geske E. Schmidt Lukas C. Müller-Kirschbaum Marcel Werner Madhobi Sen Taras Velychko Katharina Jannasch Christian Dullin Joanna Napp Klaus Pantel Harriet Wikman Maria Wiese Christof M. Kramm Frauke Alves Florian Wegwitz 《Cell death & disease》2021,12(12)
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Moschopoulou G Papanastasiou I Makri O Lambrou N Economou G Soukouli K Kintzios SE 《Plant cell reports》2007,26(12):2063-2069
We investigated a possible relationship between the levels of reactive oxygen species (ROS) and the stimulation of frond division
of the aquatic plant Spirodela polyrrhiza (duckweed) during a 7-day experimental culture period. In particular, we monitored superoxide concentration using a state-of-the-art
cell biosensor. A considerable reduction in ROS and superoxide concentration was observed during the first 2 days of culture,
whereas duckweed cultures achieved near exponential growth rates after the second day. In addition, apoptotic markers such
as the cytoplasmic concentration of cytochrome c, mitochondrial membrane depolarization and the activity of caspase-3 declined during the culture period and at least before
daughter frond maturation. We suggest that S. polyrrhiza frond division may have been stimulated by the observed reduction of free radicals and the associated avoidance of cell apoptotic
pathways in cultured plants. 相似文献
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Shima Safaiyan Simon Besson-Girard Tuğberk Kaya Ludovico Cantuti-Castelvetri Lu Liu Hao Ji Martina Schifferer Garyfallia Gouna Fumere Usifo Nirmal Kannaiyan Dirk Fitzner Xianyuan Xiang Moritz J. Rossner Matthias Brendel Ozgun Gokce Mikael Simons 《Neuron》2021,109(7):1100-1117.e10
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