全文获取类型
收费全文 | 1204篇 |
免费 | 52篇 |
专业分类
1256篇 |
出版年
2022年 | 11篇 |
2021年 | 32篇 |
2020年 | 9篇 |
2019年 | 8篇 |
2018年 | 24篇 |
2017年 | 25篇 |
2016年 | 36篇 |
2015年 | 47篇 |
2014年 | 60篇 |
2013年 | 85篇 |
2012年 | 79篇 |
2011年 | 74篇 |
2010年 | 47篇 |
2009年 | 37篇 |
2008年 | 48篇 |
2007年 | 43篇 |
2006年 | 56篇 |
2005年 | 35篇 |
2004年 | 43篇 |
2003年 | 32篇 |
2002年 | 16篇 |
2001年 | 19篇 |
2000年 | 26篇 |
1999年 | 24篇 |
1998年 | 11篇 |
1997年 | 14篇 |
1996年 | 8篇 |
1994年 | 13篇 |
1993年 | 8篇 |
1992年 | 16篇 |
1991年 | 14篇 |
1990年 | 17篇 |
1989年 | 21篇 |
1988年 | 21篇 |
1987年 | 20篇 |
1986年 | 12篇 |
1985年 | 15篇 |
1984年 | 17篇 |
1983年 | 6篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 7篇 |
1979年 | 18篇 |
1978年 | 7篇 |
1976年 | 12篇 |
1974年 | 5篇 |
1973年 | 10篇 |
1972年 | 14篇 |
1971年 | 5篇 |
1970年 | 6篇 |
排序方式: 共有1256条查询结果,搜索用时 0 毫秒
1.
The influence of wilting on the levels of free proline, soluble proteins, reducing sugars, starch and on the activities of
nitrate reductase, invertase, amylase and pyrophosphatases have been studied in the leaf tissue of five cultivars of pearl
millet at their vegetative stage under pot culture conditions. The metabolic changes could not be correlated with the yield
behaviour of the cultivars under a drought condition in the field. 相似文献
2.
3.
4.
5.
Omega-3 fatty acids increase the arachidonic acid content of liver cholesterol ester and plasma triacylglycerol fractions in the rat. 总被引:1,自引:0,他引:1 下载免费PDF全文
Recent studies have demonstrated that dietary fish oils rich in eicosapentaenoic acid (C20:5,omega 3) lower the content of arachidonic acid and its metabolites in plasma and tissue phospholipids. The present study examined the fatty acid composition of cholesterol ester and triacylglycerol fractions from plasma and livers of rats fed diets enriched with saturated fatty acids (beef tallow), alpha-linolenic acid (linseed oil) or eicosapentaenoic acid (fish oil). Feeding diets containing linseed oil or fish oil for 28 days increased arachidonic acid (C20:4,omega 6) levels in the cholesterol ester fraction of liver and in the triacylglycerol fraction of the plasma lipids. Plasma cholesterol esters were depleted of C20:4,omega 6 after feeding of the diet containing either linseed oil or fish oil. The changes in C20:4,omega 6 content cannot be explained by alterations in cholesterol ester or triacylglycerol pools of plasma and liver. These results suggest that the decrease in phospholipid C20:4,omega 6 content generally observed after fish oil consumption may be partly due to a shift of C20:4,omega 6 from phospholipid to the triacylglycerol and/or cholesterol ester pools in the same tissue. Triacylglycerols and cholesterol esters may therefore play a buffering role in the homeostatic maintenance of tissue phospholipid levels of arachidonic acid. 相似文献
6.
A non-sporulating isolate of Alternaria brassicae, inoculated on callus culture of Brassica juncea cv. Kranti, colonized the callus and produced spores. When captafol, a fungicide, was added (100 mg/l) to the callus culture medium, if effectively checked fungal contamination and saprophytic growth of A. brassicae on culture medium, without adversely affecting callus growth or establishment of dual culture. 相似文献
7.
In this paper, we describe a temperature-sensitive mutant of the yeast Saccharomyces cerevisiae (P5-9) which at a restrictive temperature (36 degrees C) shows a pleiotropic defect for transport of many different metabolites. The temperature sensitivity of the mutant is closely related to a reduction in phosphofructokinase activity. This conclusion is based on the following criteria. (i) Both the primary isolate, designated P5-9 (ts [rho-] Ino-), which is an inositol auxotroph and respiration deficient, and a purified derivative, SB4 (ts [rho+] Ino+ ), which is respiration competent and capable of growing in the absence of inositol, are temperature sensitive for growth and ethanol production in media containing glucose or fructose as the sole carbon source. (ii) The respiration-competent derivative SB4 is not temperature sensitive in media containing glycerol or glycerol-pyruvate; glucose inhibits its growth at 36 degrees C in these media. (iii) Assays of glycolytic enzymes in P5-9 and SB4 extracts, prepared from cells incubated for 1 to 2 h at 36 degrees C before harvesting, show selective reduction in phosphofructokinase activity. Analysis of tetrads derived from the cross of mutant and nonmutant haploids indicates that temperature sensitivity for growth is due to a single gene or to two closely linked genes. The biochemical analysis of spores from seven such tetrads revealed a uniform cosegregation of temperature sensitivity for growth and phosphofructokinase activity. Transport and ATP levels were drastically reduced in SB4 cells incubated at 36 degrees C for 1 to 2 h with glucose as the carbon source, but not when glycerol-pyruvate or lactate was the energy source. Therefore, depletion of energy as a result of phosphofructokinase inactivation appears to be the cause of the pleiotropic transport defect observed in the mutant. 相似文献
8.
The flag and second leaves of wheat showed physiological and biochemical differences in the normal and water stressed plants.
Although the flag leaf had lower enzyme activities in the control plants, in stressed plants it could continue nitrate assimilation
better than the second and other leaves. The flag leaf also exhibited a higher tendency to resume normal metabolic activity
after the release of stress as is indicated by higher NRA, NiRa, lower rad and rab. 相似文献
9.
An indole 2,3-dioxygenase was purified ca 38-fold from maize leaves. The enzyme had an MW of about 98000, an optimum pH of 5.0 and the energy of activation was 9.1 kcal/mol. The Kmax for indole was 1.4 × 10?4 M. The enzyme was inhibited by diethyldithiocarbamate, salicylaldoxime and sodium dithionite. The inhibition by diethyldithiocarbamate was specifically reversed by Cu2+. The dialysed enzyme was stimulated by Cu2+. Four atoms of oxygen were utilized in the disappearance of 1 mole of indole. Inhibition of the enzyme by -SH compounds and -SH group inhibitors, and their partial removal by Cu2+ only, suggested the involvement of -SH groups in binding of Cu2+ at the catalytic site. 相似文献
10.
The uptake of nutrients was investigated in the renal cortical brush border membrane (BBM) vesicles at different stages of ascending pyelonephritis. There was significant difference (p less than 0.05) in the uptake of D-glucose, L-alanine, L-aspartate, L-lysine and L-proline 3 days postinfection and onwards in both right unobstructed and left obstructed experimental kidneys as compared to the sham operated control. The uptake of D-glucose, L-lysine and L-proline was found to be significantly decreased (p less than 0.05) during the course of infection. While uptake of L-alanine and L-aspartate increased (p less than 0.05) in early stages and decreased (p less than 0.05) in later stages of infection. The differential effect was attributed to the compensatory measure and different kinds of transport systems for different types of amino acids. 相似文献