全文获取类型
收费全文 | 1756篇 |
免费 | 142篇 |
国内免费 | 1篇 |
出版年
2023年 | 10篇 |
2022年 | 20篇 |
2021年 | 24篇 |
2020年 | 17篇 |
2019年 | 16篇 |
2018年 | 29篇 |
2017年 | 18篇 |
2016年 | 46篇 |
2015年 | 91篇 |
2014年 | 76篇 |
2013年 | 134篇 |
2012年 | 138篇 |
2011年 | 130篇 |
2010年 | 88篇 |
2009年 | 85篇 |
2008年 | 111篇 |
2007年 | 100篇 |
2006年 | 96篇 |
2005年 | 82篇 |
2004年 | 85篇 |
2003年 | 72篇 |
2002年 | 78篇 |
2001年 | 21篇 |
2000年 | 21篇 |
1999年 | 31篇 |
1998年 | 20篇 |
1997年 | 17篇 |
1996年 | 19篇 |
1995年 | 16篇 |
1994年 | 11篇 |
1993年 | 6篇 |
1992年 | 11篇 |
1991年 | 9篇 |
1990年 | 7篇 |
1989年 | 9篇 |
1988年 | 14篇 |
1987年 | 7篇 |
1986年 | 8篇 |
1985年 | 6篇 |
1984年 | 6篇 |
1982年 | 5篇 |
1981年 | 7篇 |
1980年 | 9篇 |
1979年 | 7篇 |
1978年 | 12篇 |
1977年 | 6篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1971年 | 5篇 |
1967年 | 5篇 |
排序方式: 共有1899条查询结果,搜索用时 31 毫秒
1.
2.
Alteration of the Pathogenicity of Pasteurella pneumotropica for the Murine Lung Caused by Changes in Pulmonary Antibacterial Activity 总被引:7,自引:0,他引:7 下载免费PDF全文
Pasteurella pneumotropica is a potential pulmonary pathogen in mice. In healthy animals, this organism was killed rapidly by the normal function of the intrapulmonary phagocytic defense mechanisms. Impairment of this bactericidal activity by the acute renal failure of nephrectomy resulted in multiplication of the Pasteurella in the lung, both when the animals were nephrectomized first and then infected, and when the animals were infected first and nephrectomized several hours after the infection. The study demonstrates that the pathogenicity of the Pasteurella organisms is governed by the functional state of these pulmonary antibacterial mechanisms. 相似文献
3.
4.
Acute intermittent porphyria caused by a C→T mutation that produces a stop codon in the porphobilinogen deaminase gene 总被引:5,自引:1,他引:4
G. A. Scobie D. H. Llewellyn A. J. Urquhart S. J. Smyth N. A. Kalsheker P. R. Harrison G. H. Elder 《Human genetics》1990,85(6):631-634
Summary A mutation of the porphobilinogen (PBG) deaminase gene that produces the cross-reacting immunological material (CRIM)-negative type of acute intermittent porphyria (AIP) has been identified in one of 43 unrelated patients with this form of the disorder. The mutation is a CT transition that abolishes a PstI recognition site in exon 9 of the gene and converts a codon for glutamine to a stop codon. 相似文献
5.
Robert A. Holt Andrew J. Cairns J. Gareth Morris 《Applied microbiology and biotechnology》1988,27(4):319-324
Summary The pink-pigmented, amylolytic and pectinolytic bacterium Clostridium puniceum in anaerobic batch culture at pH 5.5 and 25–30°C produced butan-1-ol as the major product of fermentation of glucose or starch. The alcohol was formed throughout the exponential phase of growth and surprisingly little acetone was simultaneously produced. Furthermore, acetic and butyric acids were only accumulated in low concentrations, and under optimal conditions were completely re-utilised before the fermentation ceased. Thus, in a minimal medium containing 4% w/v glucose as sole source of carbon and energy, after 65 h at 25°C, pH 5.5 all of the glucose had been consumed to yield (g product/100 g glucose utilised) butanol 32, acetone 3 and ethanol 2. Butanol was again the major product of glucose fermentation during phosphate-limited chemostat culture wherein, although the organism eventually lost its capacity to sporulate and to synthesize granulose, production of butanol continued for at least 100 volume changes. Under no growth condition was the organism capable of producing more than 13.3 g l-1 of butanol. At pH 5.5, growth on pectin was slow and yielded a markedly lesser biomass concentration than when growth was on glucose or starch; acetic acid was the major fermentation product with lower concentrations of methanol, acetone, butanol and butyric acid. At pH 7, growth on all substrates produced virtually no solvents but high concentrations of both acetic and butyric acids. 相似文献
6.
Gareth Williams Jaswinder S Gill Vic Aber Hugh M Mather 《BMJ (Clinical research ed.)》1988,296(6617):233-235
Vibration perception threshold was measured with a biothesiometer by a single observer at both medial malleoli and both big toes in 110 diabetic patients aged 15-65 selected at random and in 64 non-diabetic subjects aged 20-65. The vibration perception threshold showed appreciable individual variation both between contralateral sites and between ipsilateral sites, differing by at least 30% between the big toes in 26 (24%) of the diabetic patients and 16 (25%) of the non-diabetic group. Variability between sites was significantly greater in the diabetics than the normal subjects. The vibration perception threshold exceeded published normal values at one or more sites in 22 of the diabetic patients but at all four sites in only four.The wide variability in vibration perception threshold among sites may be due to the tissue characteristics locally and, in diabetic patients, possibly to asymmetric neuropathy. Biothesiometer readings at single or unilateral sites may be unrepresentative or misleading. 相似文献
7.
Robert W. Lovitt Robert P. Walter J. Gareth Morris Douglas B. Kell 《Applied microbiology and biotechnology》1986,23(3-4):168-173
Summary The problem of obtaining a rapid estimate of the microbial content of an immobilised cell suspension is addressed. The low-frequency conductivity of free-living cell suspensions of Clostridium pasteurianum is lower than that of the medium in which they are suspended, by an amount conforming to the Bruggeman relation. The conductivity of the cell wall makes a negligible contribution to the measured conductivity under the conditions used. Calcium alginate beads (lacking microbial cells) lower the conductivity of a solution with which they have been equilibrated by an extent which is a function of the concentration of alginate gel used in forming the beads. When this is taken into account, the ratio of the conductivity of a suspension of gel-immobilised cells to that of the suspending medium can be used to give a rapid and convenient assessment of the amount of microbial biomass present. 相似文献
8.
FREE-FLOW ELECTROPHORETIC SEPARATION AND ELECTRICAL SURFACE PROPERTIES OF SUBCELLULAR PARTICLES FROM GUINEA PIG BRAIN 下载免费PDF全文
Continuous free-flow electrophoretic separation has been used to obtain relatively pure preparations of synaptosomes and synaptic vesicles from crude fractions of guinea pig brain homogenates. Measurements of the contents of protein, neuraminic acid, and bound acetylcholine; the activities of succinic dehydrogenase, adenosine triphosphatase, choline acetylase, and 5'-nucleotidase; and the uptake of 14C-labeled choline arid acetylcholine in the presence and absence of hemicholinium, all confirm the electron microscope evidence that the electrophoretic preparations are at least as pure as those obtained by ultracentrifugal methods. The electrophoretic mobility measurements have been used to calculate zeta potentials and surface charge densities for these particles. 相似文献
9.
10.
Gareth Lloyd 《BMJ (Clinical research ed.)》1965,1(5434):587-588