全文获取类型
收费全文 | 439篇 |
免费 | 11篇 |
国内免费 | 5篇 |
出版年
2016年 | 4篇 |
2015年 | 4篇 |
2014年 | 11篇 |
2013年 | 9篇 |
2012年 | 8篇 |
2011年 | 14篇 |
2010年 | 22篇 |
2009年 | 19篇 |
2008年 | 21篇 |
2007年 | 20篇 |
2006年 | 11篇 |
2005年 | 15篇 |
2004年 | 7篇 |
2003年 | 6篇 |
2002年 | 4篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1998年 | 12篇 |
1997年 | 17篇 |
1996年 | 8篇 |
1995年 | 11篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1991年 | 7篇 |
1990年 | 9篇 |
1989年 | 8篇 |
1988年 | 11篇 |
1987年 | 9篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 10篇 |
1979年 | 8篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1972年 | 5篇 |
1971年 | 6篇 |
1967年 | 4篇 |
1958年 | 4篇 |
1957年 | 5篇 |
1956年 | 17篇 |
1954年 | 4篇 |
1953年 | 10篇 |
1952年 | 5篇 |
1949年 | 3篇 |
排序方式: 共有455条查询结果,搜索用时 22 毫秒
1.
Tansley Review No. 112 总被引:4,自引:0,他引:4
2.
Cloning and sequencing of the peroxisomal amine oxidase gene from Hansenula polymorpha 总被引:7,自引:0,他引:7
P G Bruinenberg M Evers H R Waterham J Kuipers A C Arnberg G AB 《Biochimica et biophysica acta》1989,1008(2):157-167
We have cloned the AMO gene, encoding the microbody matrix enzyme amine oxidase (EC 1.4.3.6) from the yeast Hansenula polymorpha. The gene was isolated by differential screening of a cDNA library, immunoselection, and subsequent screening of a H. polymorpha genomic library. The nucleotide sequence of a 3.6 kilobase stretch of DNA containing the amine oxidase (AMO) gene was determined. The AMO gene contains an open reading frame of 692 amino acids, with a relative molecular mass of 77,435. The 5' and 3' ends of the gene were mapped and show that the transcribed region measures 2134 nucleotides. The derived amino-acid sequence was confirmed by sequencing an internal proteolytic fragment of the purified protein. Amine oxidase contains the tripeptide sequence Ser-Arg-Leu, located 9 residues from the carboxy terminus, which may represent the topogenic signal for protein import into microbodies. 相似文献
3.
The rate of exponential growth of Amphidinium carterae Hulburtwas the same (0.025 h1) with either or as sole N-source. Nevertheless, in short-term experiments, cells growing exponentially with as N-source took up added 56 times faster than . accumulated in the cells. Addition of inhibited, reversibly, disappearance of from the medium; prior N-deprivation of the cells did not affect this inhibition. N-repIetecells, grown with as N-source, took up for several hours both in light and darkness, butthe uptake by such cells soon ceased in darkness although it continued in light. When uptake ceased, the cells could still take up rapidly in darkness. Ammonium taken up was assimilated rapidly into organic-N includingglutamine, other amino acids and protein. Ammonium uptake in darkness was accompanied by the utilizationof cellular polysaccharide, mainly glucose polysaccharide. Mostof this carbon was unavailable for the dark assimilation of. Addition of the analogue, methylammonium, did not initiate polysacchande catabolism. Itis suggested that a control mechanism is in operation, througha product of assimilation, which operates on one or more of the enzymes concerned with polysaccharidebreakdown, for example, -amylase or phosphorylase. Uptake of was accompanied by a high rate of dark 14CO2 fixation and with both Amphidintum klebsii Kof.et Swezy and A. carterae N-deprivation led to a marked increasein this rate following addition of ; addition of had much less effect. The possible implications of these findings for the eco-physiology of marine dinoflagellatesare discussed. Key words: Ammonium, nitrate, CO2 fixation 相似文献
4.
Calli derived from seedlings of the parasitic plant Orobancheaegyptiaca were grown on enriched nutrient agar. Growth wasenhanced by the addition of 0.1 mg 11 NAA and 5 % coconutmilk. The calli developed two kinds of elongated protrusions:thin ones which resemble the young root radicle and thick oneswhich resemble the adventitious roots of Orobanche plants. Bothformed pad-like structures when touching the substrate. In liquidculture, some of the calli were observed to surround isolatedcultured tomato roots. Orobanche aegyptiaca Pers., in vitro culture, germination, coconut milk, root parasite 相似文献
5.
Carbonic Anhydrase and the Regulation of Photosynthesis 总被引:14,自引:0,他引:14
THE role of CO2 in the regulation of photosynthetic and respiratory metabolism in plants is little understood in the unicellular alga Chlorella pyrenoidosa; for example, after autotrophic growth in high CO2 (5·5% by volume), transfer to a CO2 concentration about ten times less than the concentration in air results initially in low rates of photosynthesis characterized by the virtual absence of the Calvin cycle1 of CO2 fixation2. An induction period of about 2 h is necessary before normal photosynthetic rates are established. Cells grown in air (0.03% CO2) do not show this effect and photosynthesize at comparatively high rates even in very low concentrations of CO2. 相似文献
6.
Disulfoton or phorate granules or demeton-S-methyl, menazon and vamidothion sprays, applied once in early June as preventive treatments before heavy aphid colonies developed, gave good control of Aphis fabae Scop, on field beans. Phosalone gave relatively poor results and DDT was ineffective. Applications in June to crops sown in February and early March were made with minimal wheel damage to the crop and are known to be less harmful to bees than sprays at flowering time. Eradicant treatments with demeton-S-methyl and dimethoate sprays or with disulfoton or phorate granules on heavily infested plants during flowering were also effective, but menazon was less satisfactory. These eradicant sprays are likely to be harmful to bees, and wheel damage in late June reduced yield by 1–2 cwt/acre (125–250 kg/ha). Peak populations of 3000 aphids/plant in early July reduced yield by 6 cwt/acre (750 kg/ha) in one trial. 相似文献
7.
Compared to air-breathers, animals that respire aquaticallyhave limited access to O2 and their habitats are more subjectto hypoxia. Because O2 diffuses more slowly through water thanair, animals in water experience greater diffusion boundarylayer effects on respiratory gas diffusion. While ventilationand specialized exchange surfaces mitigate O2 diffusion limitationson respiration, most animal phyla, particularly those confinedto aquatic habitats, lack these. Diffusion limitation influencesthe ontogeny of aquatic animals and may have also shaped Precambrianmetazoans. In spite of a more limited O2 access, aquatic animalsdisplay a much greater spectrum of respiratory adaptation, rangingfrom the loss of Hb in icefishes to the independent evolution,invention, and acquisition of Hb in many invertebrates confinedto hypoxic habitats. Three features of aquatic respiratory systemsdistinguishing them from aerial systems are the widespread occurrenceof integumental respiration, the frequent presence of combinedrespiratory and feeding surfaces, and the profound effect ofhypoxia on shaping respiratory adaptation, both in shallow waterand in the deep sea. 相似文献
8.
Interannual variability in phytoplankton biomass and species composition in a subtropical reservoir 总被引:6,自引:0,他引:6
- 1 The interannual variability of the dominant phytoplankton populations is described in a subtropical reservoir in Queensland using weekly data for a 16-year period between 1978 and 1994. North Pine Dam, Brisbane, is in an area characterized by strong interannual variability in rainfall. This variability is linked to El Nino Southern Oscillation (ENSO) events. Between 1978 and 1994 periods of drought (during strong ENSO events) were interspersed by periods of flooding rains. Rainfall on the catchment and temperature and oxygen within the dam showed strong 40-day periodicities which also varied in strength interannually in response to ENSO events. Similar patterns of fluctuations in the 40-day periodicity have been found elsewhere in SE Australia. Seasonal cycles of stratification in the dam were a function of both hydrographic and hydrological events. Intermittent rain storms caused partial turnovers and large outflows. As much as 90% of the dam volume was exchanged in a single flood event.
- 2 The dominant phytoplankton species were similar to those frequently found in tropical and subtropical lakes and reservoirs. The phytoplankton community switched between cyanobacterial blooms (Cylindrospermopsis, Microcystis) during drought and falling water levels and diatom blooms (Aulacoseira) in response to inflows and seasonal turnovers. There appeared to be a subtle interaction between inflows, water column stability, the periodic overturns and the occurrence of the dominant species. All the dominant species showed long periods (2–4 years) of exponential increase or decrease superimposed on top of the seasonal fluctuations in abundance. These patterns of abundance led to marked interannual variability in the phytoplankton biomass. Climate variability had a major impact on the seasonal and interannual changes of the dominant phytoplankton species.
- 3 Phytoplankton biomass tended to be depressed for about 3 months after individual storm events but the data also displayed long-term lag effects (2–4 years) which destroyed any significant correlation between water residence time and biomass. Summer maxima of biomass dominated by cyanobacteria disappeared between 1985 and 1990 and were replaced by smaller winter peaks. The data presented here are not capable of unequivocally identifying the precise reason for these longer-term effects. Because of the implications for water quality management in subtropical and tropical reservoirs they warrant further study.
9.
Axenic Cultivation of Entamoeba dispar Brumpt 1925, Entamoeba insolita Geiman and Wichterman 1937 and Entamoeba ranarum Grassi 1879 总被引:2,自引:0,他引:2
C. GRAHAM CLARK 《The Journal of eukaryotic microbiology》1995,42(5):590-593
ABSTRACT. Three species of Entamoeba have been grown in axenic culture for the first time. In two cases, novel methods for adapting the organisms to growth without bacteria were employed. While E. ranarum was axenized by the classic technique of Diamond, from a monoxenic culture with Trypanosoma cruzi as the associate, both E. dispar and E. insolita were first grown in axenic culture medium supplemented with lethally irradiated bacteria. From there, E. insolita was axenized directly, but E. dispar initially required the presence of fixed bacteria. After prolonged culture under this technically axenic but unwieldy culture system, E. dispar was eventually adapted to growth in the absence of added bacteria. 相似文献
10.
YI-S, a Casein-free Medium for Axenic Cultivation of Entamoeba histolytica, Related Entamoeba, Giardia intestinalis and Trichomonas vaginalis 总被引:2,自引:0,他引:2
LOUIS S. DIAMOND C. GRAHAM CLARK CAROL C. CUNNICK 《The Journal of eukaryotic microbiology》1995,42(3):277-278
ABSTRACT. Pancreatic digests of casein are major ingredients of media used in the axenic cultivation of lumen-dwelling parasitic protozoa, especially Entamoeba, Giardia , and trichomonads. The digest used almost exclusively in the development of these media, Medo-Peptone (Trypticase® BBL), has not been available since 1981. Moreover, none of dozens of similar type digests tested since then in our laboratory has proved equal to Medo-Peptone, and in the last two years it has become increasingly difficult to obtain new batches which will support even modest growth of Entamoeba histolytica . In response to this problem we have developed a casein-free medium, YI-S, consisting of a nutrient broth, vitamin mixture and serum. We recommend it as a replacement for the casein-dependent medium TYI-S-33, currently the most widely used for axenic culture of Entamoeba histolytica and other lumen-dwellers. 相似文献