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R Borojevic S A Vinhas A N Monteiro G B Domont F R Zyngier J A Grimaud 《Biology of the cell / under the auspices of the European Cell Biology Organization》1985,53(3):231-238
Hepatic connective tissue cells associated with schistosomal fibrosis and alcoholic cirrhosis were studied in vitro. Primary cell lines were isolated from all biopsies: they were identified as specific homogeneous cell populations, named liver connective tissue cells (LCTC). They were recognized as analogous to smooth muscle cells, different from true fibroblasts by morphological and physiological criteria. The proliferative capacity of LCTC is directly proportional to the degree of fibrosis in hepatic tissues. LCTC are able to secrete type I, III and IV collagen, fibronectin, laminin and amyloid P component. Their relationship with specific pathology of intrahepatic vascular tree in schistosomiasis is hypothesized. 相似文献
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Marie GB Hansen Mette Christoffersen Line R Thuesen Morten R Petersen Anders M Bojesen 《Acta veterinaria Scandinavica》2010,52(1):3
Background
Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum are able to infect horses. However, the extend to which Danish horses are infected and seroconvert due to these two bacteria is unknown. The aim of the present study was to evaluate the seroprevalence of B. burgdorferi sensu lato and A. phagocytophilum in Danish horses.Methods
A total of 390 blood samples collected from all major regions of Denmark and with a geographical distribution corresponding to the density of the Danish horse population were analyzed. All samples were examined for the presence of antibodies against B. burgdorferi sensu lato and A. phagocytophilum by the use of the SNAP®4DX ® ELISA test.Results
Overall, 29.0% of the horses were seropositive for B. burgdorferi sensu lato whereas 22.3% were seropositive for A. phagocytophilum.Conclusions
Antibodies against B burgdorferi sensu lato and A. phagocytophilum are commonly found among Danish horses thus showing that Danish horses are frequently infected by these organisms.4.
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Nogueira FC Gonçalves EF Jereissati ES Santos M Costa JH Oliveira-Neto OB Soares AA Domont GB Campos FA 《Plant cell reports》2007,26(8):1333-1343
Using a combination of two-dimensional gel electrophoresis protein mapping and mass spectrometry analysis, we have established
proteome reference maps of embryogenic cell suspensions of cowpea (Vigna unguiculata). The cell suspensions were generated from young primary leaves and contained basically pro-embryogenic masses, which enabled
us to dissect their proteome composition while eliminating the complexity of too many cell types. Over 550 proteins could
reproducibly be resolved over a pI range of 3–10. A total of 128 of the most abundant protein spots were excised, digested
in-gel with trypsin and analyzed by tandem mass spectrometry. This enabled the identification of 67 protein spots. Two of
the most abundant proteins were identified as a chitinase and as a ribonuclease belonging to the family of PR-4 and PR-10
proteins, respectively. The expression of the respective genes was confirmed by RT-PCR and the pattern of deposition of the
PR-10 protein in cell suspensions as well as in developing cowpea seeds, roots, shoots and flowers were determined by Western
blot experiments, using synthetic antibodies raised against a 14-amino acid synthetic peptide located close to the C-terminal
region of the PR-10 protein. 相似文献
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Daniela A. Ribeiro Danilo A. Maretto Fábio C. S. Nogueira Márcio J. Silva Francisco A. P. Campos Gilberto B. Domont Ronei J. Poppi Laura M. M. Ottoboni 《World journal of microbiology & biotechnology》2011,27(6):1469-1479
Acidithiobacillus ferrooxidans is a Gram negative, acidophilic, chemolithoautotrophic bacterium that plays an important role in metal bioleaching. During
bioleaching, the cells are subjected to changes in the growth temperature and nutrients starvation. The aim of this study
was to gather information about the response of the A.
ferrooxidans Brazilian strain LR to K2HPO4 starvation and heat stress through investigation of cellular morphology, chemical composition and differential proteome.
The scanning electron microscopic results showed that under the tested stress conditions, A. ferrooxidans cells became elongated while the Fourier transform infrared spectroscopy (FT-IR) analysis showed alterations in the wavenumbers
between 850 and 1,275 cm−1, which are related to carbohydrates, phospholipids and phosphoproteins. These findings indicate that the bacterial cell surface
is affected by the tested stress conditions. A proteomic analysis, using 2-DE and tandem mass spectrometry, enabled the identification
of 44 differentially expressed protein spots, being 30 due to heat stress (40°C) and 14 due to K2HPO4 starvation. The identified proteins belonged to 11 different functional categories, including protein fate, energy metabolism
and cellular processes. The upregulated proteins were mainly from protein fate and energy metabolism categories. The obtained
results provide evidences that A. ferrooxidans LR responds to heat stress and K2HPO4 starvation by inducing alterations in cellular morphology and chemical composition of the cell surface. Also, the identification
of several proteins involved in protein fate suggests that the bacteria cellular homesostasis was affected. In addition, the
identification of proteins from different functional categories indicates that the A. ferrooxidans response to higher than optimal temperatures and phosphate starvation involves global changes in its physiology. 相似文献
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Nogueira FC Palmisano G Schwämmle V Campos FA Larsen MR Domont GB Roepstorff P 《Journal of proteome research》2012,11(5):3046-3052
Mass spectrometry has become indispensable for peptide and protein quantification in proteomics studies. When proteomics technologies are applied to understand the biology of plants, two-dimensional gel electrophoresis is still the prevalent method for protein fractionation, identification, and quantitation. In the present work, we have used LC-MS to compare an isotopic (ICPL) and isobaric (iTRAQ) chemical labeling technique to quantify proteins in the endosperm of Ricinus communis seeds at three developmental stages (IV, VI, and X). Endosperm proteins of each stage were trypsin-digested in-solution, and the same amount of peptides was labeled with ICPL and iTRAQ tags in two orders (forward and reverse). Each sample was submitted to nanoLC coupled to an LTQ-Orbitrap high-resolution mass spectrometer. Comparing labeling performance, iTRAQ was able to label 99.8% of all identified unique peptides, while 94.1% were labeled by ICPL. After statistical analysis, it was possible to quantify 309 (ICPL) and 321 (iTRAQ) proteins, from which 95 are specific to ICPL, 107 to iTRAQ, and 214 common to both labeling strategies. We noted that the iTRAQ quantification could be influenced by the tag. Even though the efficiency of the iTRAQ and ICPL in protein quantification depends on several parameters, both labeling methods were able to successfully quantify proteins present in the endosperm of castor bean during seed development and, when combined, increase the number of quantified proteins. 相似文献
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FC Nogueira CP Silva D Alexandre RI Samuels EL Soares FJ Aragão G Palmisano GB Domont P Roepstorff FA Campos 《Proteomics》2012,12(17):2704-2715
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Cuervo P Cupolillo E Britto C González LJ E Silva-Filho FC Lopes LC Domont GB De Jesus JB 《Journal of Proteomics》2008,71(1):109-122
A comparative analysis of proteomic maps of long-term grown and fresh clinical Trichomonas vaginalis isolates exhibiting low and high virulence phenotypes, respectively, was performed using two-dimensional gel electrophoresis and mass spectrometry. Of 29 protein spots differentially expressed between the isolates, 19 were over-expressed in the isolate exhibiting high virulence phenotype: proteins associated with cytoskeletal dynamics, such as coronin and several isoforms of actin, as well as proteins involved in signal transduction, protein turnover, proteolysis, and energetic and polyamine metabolisms were identified. Some malate dehydrogenase, fructose-1,6-bisphosphate aldolase and ornithine cyclodeamidase isoforms were exclusively expressed by the highly virulent isolate. During interaction assays with VEC, parasites exhibiting high virulence phenotype rapidly adhered and switched to amoeboid forms. In contrast, low adhesion and no morphological transformation were observed in parasites displaying low virulence phenotype. Our findings demonstrate that expression of specific proteins by high and low virulence parasites could be associated with the ability of each isolate to undergo morphological transformation and interact with host cells. Such data represent an important step towards understanding of the complex interaction network of proteins that participate in the mechanism of pathogenesis of this protozoan. 相似文献
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