互花米草人工植被生态效益和经济效益的初步研究

前言在我国海涂中被日潮淹没的中、低潮带,天然生长的高等植物种类比较贫乏,分布面积也较小,许多中低潮带海滩为光滩裸地。为了绿化海滩、保护海滩,提高海滩生态系统的初级生产力,我国在1963年和1978年分别从英国     

Further Evidence for Multiple Forms of an N-Methyl-d-Aspartate Recognition Domain in Rat Brain Using Membrane Binding Techniques

Abstract— Pretreatment with sulfhydryl-reactive agents, such as N-ethylmaleimide and p-chloromercuriphenylsul-fonic acid, invariably resulted in marked inhibition of the binding of dl -(E)-2-amino-4-[³H]propyl-5-phosphono-3-pentenoic acid ([³H]CGP 39653), a competitive antagonist at an N-methyl-d -aspartate (NMDA)-sensitive subclass of central excitatory amino acid receptors, in brain synaptic membranes extensively washed and treated with Triton X-100, but did not significantly affect the binding of L-[³H]-glutamic acid ([³H]Glu), an endogenous agonist. The pre-treatment was effective in reducing the binding of [³H]-CGP 39653 at equilibrium, without altering the initial association rate, and decreased the affinity for the ligand. Pretreatment with sulfhydryl-reactive agents also enhanced the potencies of NMDA agonists to displace [³H]-CGP 39653 binding and attenuated those of NMDA antagonists, but had little effect on the potencies of the agonists and antagonists to displace [³H]Glu binding. The binding of both [³H]CGP 39653 and [³H]Glu was similarly sensitive to pretreatment with four different proteases in Tritontreated membranes, whereas pretreatment with phospho-lipase A₂ or C markedly inhibited [³H]CGP 39653 binding without altering [³H]Glu binding. Moreover, both phospho-lipases not only induced enhancement of the abilities of NMDA agonists to displace the binding of [³H]CGP 39653 and [³H]Glu, but also caused diminution of those of NMDA antagonists. These results suggest that both sulfhydryl-reactive agents and phospholipases may predominantly interfere with radiolabeling of the NMDA recognition domain in a state favorable to an antagonist by [³H]CGP 39653, with concomitant facilitation of that in an agonist-preferring form by [³H]Glu. The possible presence of multiple forms of the NMDA recognition domain is further supported by these data.     

中国种子植物区系定量化研究 V．区系相似性

本文总结了应用相似系数即关联系数进行植物区系相似性分析的现状,指出了存在的问题．然后,从集合论角度讨论了区系相似性、相似关系及其相似系数的实质．作者以为在区系相似性分析中应用Ｒ．Ｒ．Ｓｏｋａｌ和Ｃ．Ｄ．Ｍｉｃｈｅｎｅｒ（１９５８）提出的简单匹配系数比较适宜,同时亦能避免以往区系相似性分析中缺乏可比性及某些“表相”相似等问题．最后,还提出了总体相似系数和类型相似系数二个新概念,以便按照吴征镒教授关于中国植物区系研究的学术思想统一研究各个不同地区植物区系的相似性．对此,作者用了６个区系实例进行了演算说明．     

EB病毒转化Hu－TLC－SCID小鼠脾细胞的实验研究

利用ＥＢ病毒转化可产生较高水平人ＩｇＧ和特异性抗２型登革病毒人抗体的Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠脾细胞,通过免疫组化、免疫荧光和ＰＣＲ法检测转化细胞的人Ｂ细胞表面标志、ＥＢ病毒抗原和ＥＢ病毒基因。结果表明,被转化的Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠脾细胞能继续产生抗２型登革病毒的特异性人抗体,并具有人Ｂ细胞的、ＳｍＩｇＧ标志及ＥＢ病毒潜伏膜蛋白－１（ＬＭＰ－１）基因,可表达ＬＭＰ－１和ＥＢ病毒核抗原（ＥＢＮＡ）。     

旱地玉米农田棵间蒸发研究

采用中子微区模拟装置测量旱地玉农田棵间蒸发,实验表明,本实验装置实用可行。晋西棵间蒸发量占降水量（５１０ｎｍ）的５５％以上,棵间蒸发与蒸散的比值同时叶面积指数呈反相关,玉米产量与作物蒸腾呈正相关。     

原生质体电融合酵母多倍体生理特性的研究

对原生质体电融合技术获得的11株酵母多倍体融合株,进行了一系列生理特性的分析比较．结果发现,在电融合过程中,融合株的细胞体积及DNA含量并不随着核倍性呈线性递增．而是有其特殊的变化规律。当糖化酵母sta1、sta2和sta3在细胞核中各自纯合时,明显表现出表达的剂量效应。其中sta1、sta2纯合的融合株．在YEPS培养中,GA分泌可被淀粉大量诱导,表现出一定的二次生长特性。从分析结果推测,在亲株5301-14D及HU-TY—1A中可能有对GA分泌不利的因素。     

鲨鱼软骨制剂抑制血管生成的研究

以鲨鱼软骨为原料,经盐酸胍抽提,丙酮分级沉淀, 超滤等步骤得到鲨鱼软骨制剂(shark cartilage preparation,SCP). 利用整装细胞扫描电镜方法测定SCP对血管内皮细胞骨架系统的影响,体外细胞迁移实验测定它对内皮细胞迁移的抑制效应,及鸡胚绒毛尿囊膜实验测定对血管生成的抑制效应. 结果表明SCP能显著抑制内皮细胞的骨架形成;显著抑制内皮细胞的迁移,并有明显的浓度依赖关系;显著抑制鸡胚绒毛尿囊膜的血管生成. 细胞骨架是细胞分裂增殖及运动迁移的基础,血管内皮细胞的运动迁移又是血管生成的基础,因此SCP的作用机理可能是通过抑制细胞骨架的形成,抑制内皮细胞的运动迁移,从而抑制血管生成.