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Three new orosomucoid (ORM) variants revealed by isoelectric focusing and print immunofixation 总被引:2,自引:1,他引:1
Summary Phenotypes of orosomucoid (ORM) in human sera have been analysed by isoelectric focusing and print immunofixation. After neuraminidase
treatment the band patterns indicated that the polymorphism of the structural locus ORM1 is controlled by three autosomal
codominant alleles. According to the previous nomenclature they were called ORM1*F1, ORM1*F2, and ORM1*S. In a study of 272 unrelated individuals from southern Germany, five of the six expected common ORM1 subtypes were observed.
Furthermore, we found three ORM variant phenotypes which have not been reported previously. These variants were characterized
by additional bands in a cathodal position. One variant had additional double bands and presumably represents a rare ORM1
variant named ORM1S1. Two variants had additional single bands. They were assigned tentatively to the ORM2 gene locus. While
the common gene product of ORM2 may be called ORM2A, the two variants are named ORM2B1 and ORM2B2, respectively. ORM2B1 has,
thus far, been found only in a single individual; the variants ORM1S1 and ORM2B2 were found in a father-child pair and a mother-child
pair, respectively. The frequency for variants tentatively assigned to the ORM2 locus is very low and was calculated to be
0.0037. 相似文献
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Two "new" phenotypes of the esterase D system, named EsD 4-1 and EsD 4-2, were observed in a father and his daughter, respectively. An additional allele EsD4 is postulated. 相似文献
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Song YS Hepting L Schweizer G Hartl L Wenzel G Schwarzfischer A 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(5):879-887
The inheritance of extreme resistance to PVY (Ry
sto) by a single dominant locus was confirmed by obtaining a 1:1 segregation ratio in a virus inoculation test with 28 resistant
(Ryry) to 29 susceptible (ryry) anther culture-derived dihaploid lines (2n=2x=24) from cv. “Assia” (2n=4x=48) having extreme resistance derived from Solanum stoloniferum in simplex constitution (Ryryryry). Twelve Ry
sto markers selected in AFLP assays using bulked segregant analysis were applied to 106 tested potato cultivars from Germany,
The Netherlands and Poland and 19 potato cultivars were identified by these markers as extremely resistant to PVY in alignment
with phenotypic data. The locus for extreme resistance (Ry
sto) to PVY was mapped on chromosome XII co-segregating with the SSR marker STM0003. The utility of anther-culture derived dihaploid
potatoes for genetic marker development was demonstrated. Marker transferability from diploids to tetraploids provides an
optimistic potential for marker-assisted selection in potato breeding programs. 相似文献
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Gebhardt C Bellin D Henselewski H Lehmann W Schwarzfischer J Valkonen JP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(8):1458-1464
Closely linked PCR-based markers facilitate the tracing and combining of resistance factors that have been introgressed previously into cultivated potato from different sources. Crosses were performed to combine the Ry
adg
gene for extreme resistance to Potato virus Y (PVY) with the Gro1 gene for resistance to the root cyst nematode Globodera rostochiensis and the Rx1 gene for extreme resistance to Potato virus X (PVX), or with resistance to potato wart (Synchytrium endobioticum). Marker-assisted selection (MAS) using four PCR-based diagnostic assays was applied to 110 F1 hybrids resulting from four 2× by 4× cross-combinations. Thirty tetraploid plants having the appropriate marker combinations were selected and tested for presence of the corresponding resistance traits. All plants tested showed the expected resistant phenotype. Unexpectedly, the plants segregated for additional resistance to pathotypes 1, 2 and 6 of S. endobioticum, which was subsequently shown to be inherited from the PVY resistant parents of the crosses. The selected plants can be used as sources of multiple resistance traits in pedigree breeding and are available from a potato germplasm bank. 相似文献