全文获取类型
收费全文 | 1292篇 |
免费 | 127篇 |
国内免费 | 1篇 |
专业分类
1420篇 |
出版年
2023年 | 5篇 |
2022年 | 13篇 |
2021年 | 26篇 |
2020年 | 20篇 |
2019年 | 17篇 |
2018年 | 24篇 |
2017年 | 31篇 |
2016年 | 28篇 |
2015年 | 54篇 |
2014年 | 81篇 |
2013年 | 81篇 |
2012年 | 95篇 |
2011年 | 80篇 |
2010年 | 62篇 |
2009年 | 55篇 |
2008年 | 59篇 |
2007年 | 67篇 |
2006年 | 62篇 |
2005年 | 63篇 |
2004年 | 45篇 |
2003年 | 52篇 |
2002年 | 37篇 |
2001年 | 34篇 |
2000年 | 26篇 |
1999年 | 22篇 |
1998年 | 18篇 |
1997年 | 15篇 |
1996年 | 16篇 |
1995年 | 11篇 |
1994年 | 9篇 |
1993年 | 15篇 |
1992年 | 15篇 |
1991年 | 15篇 |
1990年 | 12篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 12篇 |
1986年 | 10篇 |
1985年 | 9篇 |
1984年 | 16篇 |
1983年 | 6篇 |
1982年 | 7篇 |
1981年 | 8篇 |
1980年 | 6篇 |
1979年 | 13篇 |
1977年 | 4篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1971年 | 4篇 |
1967年 | 6篇 |
排序方式: 共有1420条查询结果,搜索用时 15 毫秒
1.
2.
3.
Phospholipase activities of rat intestinal mucosa homogenate have been determined from lysophosphatidylcholines [14C] and phosphatidylcholines [-3H-14C]. In the presence of phosphatidylcholines, at pH 6.5, the homogenate has a phospholipase B activity. At pH 8.5, a phospholipase A2 activity was shown. In the presence of lysophospatidylcholines, at pH 6.5, we notice a lysophospholipase A1 activity. A kinetic study of the reactions allows us to separate the activity B into a phospholipase A2 activity and a lysophospholipase A1 activity. Thus, it appears that the total phospholipase activity of rat intestinal mucosa would results from a phospholipase A2 activity and a lysophospholipase A1 activity. 相似文献
4.
Ludo Filez Willy Stalmans Freddy Penninkx Raymond Kerremans Karel Geboes 《Bioscience reports》1987,7(12):917-923
Lactate dehydrogenase has been measured in the small-intestinal mucosa in order to assess its value as a marker for the effects of ischemia and of reperfusion. The decrease in specific activity of the enzyme illustrates the deleterious effect of reperfusion on the quality of the remaining epithelial cells. However, this parameter fails to detect the loss of epithelial cells, which is the major event during ischemia as well as during reperfusion. In contrast, the expression of enzyme activity per g protein of the underlying intestinal muscle allowed us, in addition, to assess quantitatively the loss of epithelial cells, in good agreement with the histological data. 相似文献
5.
Freddy Bugge Christiansen 《Journal of genetics》1987,66(1):45-67
The balance between the creation of associations between alleles at different loci by immigration and the convergence to linkage equilibrium due to the recombination process is studied in a theoretical model. The geographical structure of the model is a stepping-stone chain of populations linking two genetically constant source populations. The model assumes an arbitrary number of autosomal loci and considers genetic variation (two alleles at each locus) that is not subject to natural selection. The gene frequencies at each locus will then show a linear cline through the stepping-stone chain of populations. The deviation from linkage equilibrium through the stepping-stone cline is characterized by an equation for linear measures that provide the linkage disequilibrium measures for a given set of loci in terms of the gene frequencies and the linkage disequilibria in the source populations and in terms of the linkage disequilibrium measures through the cline for lower numbers of loci. Numerical examples of this iterative solution are given, and it is shown that the build-up of the higher order Bennett-disequilibria through the cline is considerably more pronounced than the build-up of two-locus disequilibria. 相似文献
6.
A. E. Schwint B. M. de Rey O. A. Bernaola R. Mazzei M. E. Itoiz 《Radiation and environmental biophysics》1989,28(2):121-130
Summary Rat tail epidermis was used to analyze the in vivo response of a biological system to heavy particle irradiation. The conical configuration of the rat tail gives rise to a variable energy degradation of the beam thus yielding information on the damage elicited by 2 different L.E.T. regions of the helium beam at different sites on the same sample. Cytochrome oxidase activity and epidermal thickness were used to analyze the metabolic and structural radioinduced response. Quantitative evaluation of radiation damage revealed marked variations within a few micrometers of tissue. 相似文献
7.
Atrazine and diuron resistant clones were isolated from diploid photoautotrophic protoplastderived colonies of Nicotiana plumbaginifolia. Protoplasts were mutagenised with 0.1 mM N-ethyl-N-nitrosourea and colonies were screened for resistance after plating. Selection of calli was carried out on their ability to grow and green on a selective medium containing either atrazine or diuron. Plants were regenerated from most tolerant calli. Herbicide spray showed that plants of 6 and 4 clones were resistant to atrazine and diuron, respectively.Abbreviations Atrazine
2-chloro-4-ethylamino-6-isopropyl-amino-s-triazine
- diuron
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- NEU
N-ethyl-N-nitrosourea
- PSII
photosystem II 相似文献
8.
Characterization of granzymes A and B isolated from granules of cloned human cytotoxic T lymphocytes 总被引:14,自引:0,他引:14
O Kr?henbühl C Rey D Jenne A Lanzavecchia P Groscurth S Carrel J Tschopp 《Journal of immunology (Baltimore, Md. : 1950)》1988,141(10):3471-3477
A human CD8+ CTL clone with cytolytic potential was shown to express two serine proteases, a 50-kDa homodimer and a 27-kDa monomer, which were purified from cytoplasmic granules. N-terminal sequencing of the purified proteins revealed that the 50-kDa homodimer is the gene product of the human Hanukah factor cDNA clone and that it represents the human homologue to granzyme A. Similarly, the 27-kDa protein was shown to be the serine esterase encoded by the human lymphocyte protease cDNA clone and corresponds to granzyme B. There was no evidence for the presence of other granzymes, in particular for the human homologues to murine granzymes C, D, E, and F. The substrate best cleaved by granzyme A was Gly-Pro-Arg-amido-4-methyl-coumarin after the Arg residue and the pH optimum was 8 to 8.5. Upon triggering of the TCR-CD3 complex with an anti-CD3 mAb, granzyme A was released into the culture medium. Furthermore, a granule-associated hemolytic activity was detected after salt extraction and partial purification of granule proteins. This suggests that hemolytically active human perforin can be obtained from inactive granules. 相似文献
9.
Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and BAP, respectively. Shoots formed roots upon transfer to MS with 0.01 mg/l NAA. Plantlets were succesfully transferred to soil. Leaf-derived calli of Centrosema arenarium, C. macrocarpum, C. pascuorum, C. pubescens, and C. virginianum did not produce shoots when cultured in vitro. 相似文献
10.
A Rey B Klein M Rucheton J Caraux D Zagury C Thierry B Serrou 《Cellular immunology》1984,86(1):155-164
Peripheral blood lymphocytes (PBL) of solid-tumor-bearing cancer patients produced a lower interleukin 2 (IL-2) activity after lectin stimulation than did those from normal subjects. Moreover natural killer (NK) cell activity and autologous rosette forming (ARF) cell rate are found significantly correlated with IL-2 production in these patients. No direct relation is observed between ARF cell ratio and NK cell activity in a given patient. A central role for IL-2 in cancer patient immune dysfunctions is suggested. Two lines of pathogenetic mechanisms are documented. First, PBL exhibited cellular function defects, namely, autologous receptor expression, IL-2 production, and NK activity. Second, these dysfunctions involved, at least partly, plasma factors. The possibility of specific deficiency, (e.g., thymic factors) is not documented. Conversely it is demonstrated that patient plasma contain immunosuppressive factor(s) that block(s) IL-2 production and ARF cell expression. Involvement of ARF cell receptor in T-cell activation is discussed. 相似文献