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1.
E. Jay DeRocher Francoise Quigley Regis Mache Hans J. Bohnert 《Molecular genetics and genomics : MGG》1993,239(3):450-462
The nucleotide sequences of the entire gene family, comprising six genes, that encodes the Rubisco small subunit (rbcS) multigene family in Mesembryanthemum crystallinum (common ice plant), were determined. Five of the genes are arranged in a tandem array spanning 20 kb, while the sixth gene is not closely linked to this array. The mature small subunit coding regions are highly conserved and encode four distinct polypeptides of equal lengths with up to five amino acid differences distinguishing individual genes. The transit peptide coding regions are more divergent in both amino acid sequence and length, encoding five distinct peptide sequences that range from 55 to 61 amino acids in length. Each of the genes has two introns located at conserved sites within the mature peptide-coding regions. The first introns are diverse in sequence and length ranging from 122 by to 1092 bp. Five of the six second introns are highly conserved in sequence and length. Two genes, rbcS-4 and rbcS-5, are identical at the nucleotide level starting from 121 by upstream of the ATG initiation codon to 9 by downstream of the stop codon including the sequences of both introns, indicating recent gene duplication and/or gene conversion. Functionally important regulatory elements identified in rbcS promoters of other species are absent from the upstream regions of all but one of the ice plant rbcS genes. Relative expression levels were determined for the rbcS genes and indicate that they are differentially expressed in leaves. 相似文献
2.
Robert R. Crichton Francoise Roman Francine Roland 《Journal of inorganic biochemistry》1980,13(4):305-316
The release of iron from horse spleen ferritin by the chelating agents desferrioxamine B, rhodotorulic acid, 2,3-dihydroxybenzoate, 2,2′-bipyridyl and pyridine-2-aldehyde-2-pyridyl hydrazone (Paphy) has been studied in vitro. Ferritin prepared by classical procedures involving thermal denaturation releases its iron less effectively than ferritin isolated by a modified procedure that avoids this step. Desferrioxamine B and rhodotorulic acid are the most effective in releasing iron from both preparations of ferritin. When FMN is added, iron release by desferrioxamine B, rhodotorulic acid, and 2,3-dihydroxybenzoate was effectively blocked, whereas both bipyridyl and Paphy showed a marked simulation. A substantial increase in iron release was also observed for bipyridyl and Paphy with ascorbate; a less important increase was noted for rhodotorulic acid. EDTA exerted a marked inhibition of iron release from ferritin with rhodotorulic acid, 2,3-dihydroxybenzoate, bipyridyl, and Paphy. The effects of citrate and oxalate on iron release by the chelators was small. The effect of the concentration of flavin on iron release from ferritin by bipyridyl and desferrioxamine B have been studied. Desferrioxamine is unable to mobilize FeII from ferritin following reduction by reduced FMN, whereas bipyridyl can rapidly complex the ferrous iron. The results are discussed in the context of our current concepts of storage iron mobilization in the treatment of iron overload. 相似文献
3.
Variations in litter size and reproductive effort within and between some populations of Lacerta vivipara 总被引:1,自引:0,他引:1
We studied the relationships between litter size, litter weight, newborn weight, relative clutch mass and the female snout-vent length in some Lacerta vivipara populations over a period of three years.
Litter size and litter weight were positively correlated with female snout-vent length in all the populations for all the years, as in most other lizard species. Relative clutch mass generally increased with female size, though correlations appear not to be very tight.
Considering the two best studied populations suggests that montane females invest less in reproduction than lowland ones.
The main reproductive traits of the species appeared highly variable between as well as within the different populations hitherto studied.
We argue that current theory about lizard reproductive strategy requires, first to work out a good estimate of reproductive effort, and second to get more information about the relations between the species and their environmental, biotic and abiotic conditions. 相似文献
Litter size and litter weight were positively correlated with female snout-vent length in all the populations for all the years, as in most other lizard species. Relative clutch mass generally increased with female size, though correlations appear not to be very tight.
Considering the two best studied populations suggests that montane females invest less in reproduction than lowland ones.
The main reproductive traits of the species appeared highly variable between as well as within the different populations hitherto studied.
We argue that current theory about lizard reproductive strategy requires, first to work out a good estimate of reproductive effort, and second to get more information about the relations between the species and their environmental, biotic and abiotic conditions. 相似文献
4.
Marc Lutz Ileana Agalidis Guy Hervo Richard J. Cogdell Francoise Reiss-Husson 《BBA》1978,503(2):287-303
The carotenoids bound to reaction centers of wild, Ga and GlC strains of Rhodopseudomonas spheroides, of Rhodospirillum rubrum, strain S1 and of Rhodopseudomonas viridis, yield very similar, but unusual resonance Raman spectra. Through a comparison with resonance Raman spectra of 15,15′-cis-β-carotene, these carotenoids are shown to assume cis conformations, while the corresponding chromatophores contain all-trans forms only. These cis conformations likely are identical for all the carotenoids studied. They remain unaffected by variations of temperature from 20 to 300 K as well as by the redox state of . They are unstable, being rapidly isomerised towards the all-trans forms when extracted from the reaction centers. The possible nature of these conformers is discussed on the basis of their electronic and vibrational spectra. 相似文献
5.
Claire-Michelle Calberg-Bacq Camille Francois Luc Gosselin Paul M. Osterrieth Francoise Renteir-Delrue 《生物化学与生物物理学报:生物膜》1976,419(3):458-478
Milk fat globule membranes and mammary tumour virus particles (d = 1.17 g/cm3) have been obtained from the milk of a Swiss albino mice strain. Comparitive biochemistry shows that these two structures differ significantly in the phospholipid, polypeptide and glycopolypeptide patterns and enzymatic activities. However, the lipid profile and the morphology of both structures suggest a filiation with the plasma membrane. Density fractions obtained from the crude virus preparation have been thoroughly investigated. The results suggest that most of these fractions represent degraded virus and/or atypical virus assembly. 相似文献
6.
Robin E. Everts Serge A. Versteeg Corinne Renier Francoise Vignaux Peter C. Groot Jan Rothuizen Bernard A. van Oost 《Mammalian genome》2000,11(9):741-747
Genomic Representational Difference Analysis (gRDA) is a subtractive DNA method to clone the differences between two related
genomes, called tester and driver. We have evaluated this method to obtain polymorphic DNA markers for pedigree dogs. Amplified
size-selected genomic restriction fragments (amplicons) of two dog littermates were repeatedly hybridized to each other in
order to remove (subtract) those restriction fragments common to both sibs. Already after two rounds of subtractive hybridization,
a clear enrichment of presumably tester-specific restriction fragments was observed, which was even more pronounced after
the third round of subtraction. A plasmid library of 3000 recombinant clones was constructed of the second round and of the
third round difference product. DNA sequence determination of randomly chosen clones of each difference product showed that
approximately 1000 unique clones were obtained in the second-round difference product and approximately 500 in the third-round
difference product. About half of the clones identified in the second-round difference product were also present in the third-round
difference product. Of the second-round difference product, 39 different gRDA fragments could be identified, of which 21 were
tester specific. In the third-round difference product, 22 different gRDA fragments were identified, of which 18 were tester
specific. There were 13 fragments in common, resulting in a total of 48 different fragments. In order to establish the localization
of these markers, we performed mapping using the dog radiation hybrid panel RHDF5000. Of 39 mapped clones, 29 were mapped
to 20 existing RH groups, and 10 remained unlinked. It is concluded that gRDA is suitable to generate DNA markers to track
disease genes within lines of pedigree dogs.
Received: 26 April 2000 / Accepted: 11 May 2000 相似文献
7.
Jeremie Boyer Vania Bernardes-Genisson Vincent Farines Jean-Pierre Souchard Francoise Nepveu 《Free radical research》2013,47(5):459-471
A series of 2-alkyl and 2-aryl substituted-3H-indol-3-one-1-oxides was prepared and evaluated for its radical trapping properties. Spin trapping and electron paramagnetic resonance experiments demonstrate the ability of these indolone-1-oxides to trap hetero- and carbon-centered radicals. The most stable spin adducts (lifetime of several hours) are obtained with 2-alkyl substituted nitrones, the 2-ethyl-5,6-dioxolo-3H-indolone-1-oxide, 5e and the 2-secbutyl-3H-indolone-1-oxide, 5f. These two nitrones are also sensitive to redox reactions in solution. Therefore this indolone-1-oxide series lacking a β-hydrogen atom gives rise to highly stable adducts with free radicals. 相似文献
8.
Constance Delaby Vincent Oustric Caroline Schmitt Francoise Muzeau Anne-Marie Robreau Philippe Letteron Eric Couchi Angel Yu Saïd Lyoumi Jean-Charles Deybach Herve Puy Zoubida Karim Carole Beaumont Bernard Grandchamp Peter Demant Laurent Gouya 《Mammalian genome》2013,24(11-12):427-438
Disorders of iron metabolism are among the most common acquired and constitutive diseases. Hemochromatosis has a solid genetic basis and in Northern European populations it is usually associated with homozygosity for the C282Y mutation in the HFE protein. However, the penetrance of this mutation is incomplete and the clinical presentation is highly variable. The rare and common variants identified so far as genetic modifiers of HFE-related hemochromatosis are unable to account for the phenotypic heterogeneity of this disorder. There are wide variations in the basal iron status of common inbred mouse strains, and this diversity may reflect the genetic background of the phenotypic diversity under pathological conditions. We therefore examined the genetic basis of iron homeostasis using quantitative trait loci mapping applied to the HcB-15 recombinant congenic strains for tissue and serum iron indices. Two highly significant QTL containing either the N374S Mon1a mutation or the Ferroportin locus were found to be major determinants in spleen and liver iron loading. Interestingly, when considering possible epistatic interactions, the effects of Mon1a on macrophage iron export are conditioned by the genotype at the Slc40a1 locus. Only mice that are C57BL/10ScSnA homozygous at both loci display a lower spleen iron burden. Furthermore, the liver-iron lowering effect of the N374S Mon1a mutation is observed only in mice that display a nonsense mutation in the Ceruloplasmin (Cp) gene. This study highlights the existence of genetic interactions between Cp, Mon1a, and the Slc40a1 locus in iron metabolism, suggesting that epistasis may be a crucial determinant of the variable biological and clinical presentations in iron disorders. 相似文献
9.
10.