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Keith G. Danielson Janice E. Knepper Frances S. Kittrell Janet S. Butel Daniel Medina Elisa M. Durban 《In vitro cellular & developmental biology. Plant》1989,25(6):535-543
Summary Clonal populations were isolated from the mouse mammary cell line, COMMA-D, by transfection with a dominant-selectable gene,
pSV2Neo, which confers resistance to the antibiotic, G418. Seven of twenty-four clones isolated retained the ability of the
parental line to repopulate cleared mammary fat pads in vivo as ductal-alveolar hyperplasias. Two sublines designated CDNR2
and CDNR4 retained hyperplastic growth potential after multiple passages in vitro with low incidence of tumor formation. A
third subpopulation, CDNR1, contained a single integration site for the pSV2Neo plasmid indicating a bonafide clonal origin
for this subline. CDNR1 cells displayed heterogeneous growth phenotypes in vivo including hyperplasia, adenocarcinoma, and
bone formation. Functional differentiation of CDNR1 cells organized as alveolarlike structures in vivo or on floating collagen
gels in vitro was observed as determined by immunoperoxidase staining for the milk-specific protein, casein. Overall, the
results indicate that a subset of cells from the COMMA-D cell line may be functionally analogous to stem cells existing in
the mammary gland.
Supported by NCI research grants CA-38650, CA-33369, CA-39017, and CA-25215. 相似文献
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E. coli cells containing a temperature-sensitivednaE mutation, in the α-subunit of holoenzyme DNA polymerase III, do not survive at the restrictive temperature. Such cells may
survive in the presence of thepcbA1 mutation, an allele of thegyrB gene. Such survival is dependent on an active DNA polymerase I. Evidence indicates that DNA polymerase I interacts directly
in the replisome (REP·A). Despite normal survival for cells using thepcbA replication pathway after some type of DNA damage, we have noted a failure of damage-induced mutagenesis. Here we present
evidence supporting a model of replisome pausing in cells dependent upon thepcbA replication pathway. The model argues that the (REP·A) complex pauses longer at the site of the lesion, allowing excision
repair to occur completely. In the normal replication pathway (REP·E) bypass of the lesion occurs, fixing the mutation. 相似文献
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Abstract The effects of an unusual high frequency mowing regime, which involved the removal of slash, were compared to moderate grazing through the method of paired quadrats across a fenceline, which was orthogonal to a weak environmental gradient. The mown plots proved superior in their conservation characteristics to the moderately grazed plots. The mowing regime produced greater cover of rare or threatened species, greater native cover and lesser exotic grass cover. It thus presents an opportunity for maintaining or improving the condition of previously grazed remnants in reserves without resorting to the use of stock or fire for biomass reduction. 相似文献
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