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In this study, we report the screening of four Arcobacter species for the presence of small and large plasmids. Plasmids were present in 9.9% of the 273 examined strains. One Arcobacter cryaerophilus and four Arcobacter butzleri plasmids were selected for further sequencing. The size of three small plasmids isolated from A. butzleri and the one from A. cryaerophilus strains ranged between 4.8 and 5.1 kb, and the size of the large plasmid, isolated from A. butzleri, was 27.4 kbp. The G+C content of all plasmids ranged between 25.4% and 26.2%. A total of 95% of the large plasmid sequence represents coding information, which contrasts to the 20 to 30% for the small plasmids. Some of the open reading frames showed a high homology to putative conserved domains found in other related organisms, such as replication, mobilization and genes involved in type IV secretion system. The large plasmid carried 35 coding sequences, including seven genes in a contiguous region of 11.6 kbp that encodes an orthologous type IV secretion system found in the Wolinella succinogenes genome, Helicobacter pylori and Campylobacter jejuni plasmids, which makes this plasmid interesting for further exploration.  相似文献   
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Densities of Drosera intermedia were low in two studied habitats (10–25 ramets m−1), a path through a wet heath (short inundation in spring, low soil moisture in summer) and a pool edge (longlasting inundation, high soil moisture in summer). The low densities could be explained by the observed low recruitment and high adult mortality.
The low recruitment resulted from: (1) a high first year mortality of the large number of seedlings that emerged each year in the path population, caused by summer drought and cover with algae after heavy rainfall; (2) the absence in two years out of three of seedling emergence at the pool edge, due to the longlasting inundation. In neither population any seedlings survived to flower; (3) low vegetative reproduction rate.
Adult mortality during the growing season was caused by drought, which did not occur at the pool edge. Rapid senescence in autumn, caused by summer drought on the path and by a rapid submersion after heavy rainfall at the pool edge, was associated with a high winter mortality.  相似文献   
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Summary Are there underlying developmental and physiological properties of organisms that can be used to build a general theory of life history evolution? Much of the theoretical work on the evolution of life histories is based on the premise of negative developmental and genetic correlations among life history traits. If negative correlations do not exist as a general rule then no general theory taking them into account is possible. Negative genetic correlations among life history traits can come about by antagonistic pleiotropy. One cause of antagonistic pleiotropy is cost allocation trade-offs. Since cost allocation trade-offs are due to underlying physiological constraints they are expected to be common to closely related groups. A second form of antagonistic pleiotropy is specialization of genotypes to different niches. This type of antagonistic pleiotropy is expected to be specific to each population. We looked for trade-offs in life history traits of longevity and fecundity inDrosophila melanogaster. We used a half-sib mating design and raised the offspring at two temperatures, 19°C and 25°C. Correlations between longevity and fecundity showed some evidence of antagonistic pleiotropy at high temperature with no evidence of any trade-offs at low temperature. Correlations of early and late fecundity traits did show evidence of cost allocation trade-offs at both temperatures. Antagonistic pleiotropy was also found for cross-environmental correlations of fecundity traits. We conclude that, although life history trade-offs can not be generally assumed, they are frequently found among functionally related traits. Thus, we provide guidelines for the development of general theories of life history evolution.  相似文献   
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Pollen of the white birch (Betula verrucosa) is one of the main causes of Type I allergic reactions (allergic rhinoconjunctivitis, allergic bronchial asthma) in Middle and Northern Europe, North America and the USSR. Type I allergies are a major threat to public health in these countries, since 10-15% of the population suffer from these diseases. BetvI, an allergenic protein with an Mr of 17 kd is a constituent of the pollen of white birch and is responsible for IgE binding in more than 95% of birch pollen allergic patients. Here, we report the complete nucleotide sequence and deduced amino acid sequence of a cDNA clone coding for the major pollen allergen (BetvI) of white birch. It is similar to the N-terminal peptide sequences of the allergens of hazel, alder and hornbeam (close relatives) but it has no significant sequence homology to any other known allergens. However, it shows 55% sequence identity with a pea disease resistance response gene, indicating that BetvI may be involved in pathogen resistance of pollen.  相似文献   
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The reproductive behaviour of the longleaved sundew Drosera intermedia Hayne was studied in three hydrologically differing heathland habitats a pool edge, an old path through the wet heath and a seepage area
Sexual reproductive allocation (SRA) (the slope of the regression of sexual biomass on vegetative biomass m a given season) differed strongly between years (related to temperature and precipitation) and habitats Averaged over years, path plants had a higher SRA than plants in the seepage area and at the pool edge
Asexual reproductive allocation, expressed as the size-dependent probability of reproducing asexually, did not differ between habitats in most years
The observations agreed with general life history theory SRA was high in the path habitat where adult survival chances were low and juvenile (= seed, first year seedling) survival was high SRA was lower in the seepage area where adult survival chances were higher and juvenile survival lower At the pool edge, a habitat with high adult mortality and low juvenile establishment, the predicted optimal reproductive behaviour, a high SRA, was not observed due to environmental constraints (inundation)  相似文献   
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Among the unicellular protists, several of which are parasitic, some of the most divergent eukaryotic species are found. The evolutionary distances between protists are so large that even slowly evolving proteins like histones are strongly divergent. In this study we isolated cDNA and genomic histone H3 and H4 clones fromTrichomonas vaginalis. Two histone H3 and three histone H4 genes were detected on three genomic clones with one complete H3 and two complete H4 sequences. H3 and H4 genes were divergently transcribed with very short intergenic regions of only 194 bp, which containedT. vaginalis-specific as well as histone-specific putative promoter elements. Southern blot analysis showed that there may be several more histone gene pairs. The two complete histone H4 genes were different on the nucleotide level but encoded the same amino acid sequence. Comparison of the amino acid sequences of theT. vaginalis H3 and H4 histones with sequences from animals, fungi, and plants as well as other protists revealed a significant divergence not only from the sequences in multicellular organisms but especially from the sequences in other protists likeEntamoeba histolytica, Trypanosoma cruzi, andLeishmania infantum.  相似文献   
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