Kinetics of 14C-cholic acid in the baboon under normal conditions and in cholestasis. Description and validity of a multicompartmental model]

A multicompartmental model was applied to the study of the plasmatic and biliary kinetics of the 14C-Cholic acid intravenously injected into a baboon in normal and cholestatic condition. For the evaluation of transfer rates FORTAN IV procedures were used, utilizing Powell method. The degree of fitting was: in normal condition in serum 7% for free and 35% for conjugated Cholic acid, while in bile 5% and 4% respectively; in serum in cholestatic condition 7% for free and 12% for conjugates. The high degree of fitting and reliable estimation of transfer rates suggest that the multicompartmental model applied represents most likely the physio-pathological conditions studied.     

A radioimmunoassay for lithocholic acid conjugates in human serum and liver tissue

A sensitive and specific radioimmunoassay for glycine and taurine conjugates of lithocholic acid (CLCA) has been developed. ³H-glycolithocholic acid (S.A. = 17Ci/mmol) was used as tracer. Separation of free from antibody-bound bile acid was carried out using ammonium sulphate (saturated solution). The antiserum showed high specificity for both glyco and tauro conjugated lithocholate (100% cross reaction) and lithocholic acid (25% cross reaction). The sensitivity of the assay (1 pmole/tube), was adequate for measuring CLCA in peripheral blood and hepatic tissue in man.     

Plasma antioxidant enzymes and clastogenic factors as possible biomarkers of colorectal cancer risk

Oxidative damage plays an important role in the pathogenesis of colorectal (CR) cancer. This study investigated the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S-transferase (GST) in plasma of 82 participants of a screening program for CR cancer prevention (30 females and 52 males; age 50-70 years). All subjects resulted positive to fecal occult blood test and were subsequently classified, according to the colonoscopy and histological findings, in patients with CR cancer, patients with colorectal polyps or controls. Furthermore, the activity of clastogenic factors (CFs) in plasma from study population was measured as the ability of inducing micronuclei (MN) in vitro in peripheral of a healthy donor. CAT and GR activities were significantly lower in CR cancer patients compared to controls (P<0.05) and polyps groups (P<0.05). SOD activity was significantly higher in patients with CR cancer than in polyp (P<0.05) and control (P<0.05) groups. GST activity was not significantly different in plasma of the three groups. An increase of CFs induction was observed in plasma of CR cancer patients (MN: 8.89±3.42) with respect to control (MN: 6.37±0.96 P<0.05). These results can contribute to define plasma biomarkers associated to oxidative stress damage that could predictive of CR cancer risk.     

The Late Neolithic settlement of Latsch, Vinschgau, northern Italy: subsistence of a settlement contemporary with the Alpine Iceman, and located in his valley of origin

Archaeobotanical and archaeozoological analyses conducted on Late Neolithic settlement layers at Latsch provide the first data on the subsistence strategy, diet and environmental conditions in the valley of origin of the Alpine Iceman. The results prove that during the Late Neolithic, the valley was occupied by an agro-pastoral society based on the cultivation of Hordeum vulgare (hulled barley), Triticum monococcum (einkorn), T. dicoccum (emmer) and Pisum sativum (pea), as well as on the rearing of Ovis vel Capra, Ovis orientalis f. aries (sheep), Capra aegagrus f. hircus (goat) and Bos primigenius f. taurus (cattle). The settlement was located on a river terrace of the valley floor close to a river and was surrounded by mixed pine and oak woods beside alluvial woodland.     

Holocene vegetation history and human impact in the eastern Italian Alps: a multi-proxy study on the Coltrondo peat bog,Comelico Superiore,Italy

Vegetation History and Archaeobotany - The present study aims to reconstruct vegetation development, climate changes and human impact using an ombrotrophic peat core from the Coltrondo bog in the...