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In an attempt to increase tolerance to salinity stress in tobacco plants, the genes encoding the mutant form of glutamyl kinase
(proB), pyrroline-5-carboxylate synthetase, and osmotin were cloned into three different shuttle vectors and were separately introduced
into the tobacco plants. The transgenic lines were compared for their ability to produce shoots and grow in MS medium containing
320 mM NaCl; it was shown that the transgenic lines containing genetically handled osmotin gene are more resistant to salinity. The amount of chlorophyll a was used to show continuing growth of plant lines. The results showed that only the tobacco lines transformed with the modified
osmotin gene exhibited greater tolerance to salinity.
Published in Russian in Fiziologiya Rastenii, 2006, Vol. 53, No. 1, pp. 122–127.
The text was submitted by the authors in English. 相似文献
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Oghbaei Hajar Hosseini Leila Farajdokht Fereshteh Rahigh Aghsan Sepideh Majdi Alireza Sadigh-Eteghad Saeed Sandoghchian Shotorbani Siamak Mahmoudi Javad 《Molecular biology reports》2021,48(8):5881-5887
Molecular Biology Reports - The current study was set to assess the effect of heat stress exposure on oxidative stress, apoptosis, and endoplasmic reticulum stress markers in the cerebellum of male... 相似文献
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Seyed Nezamedin Hosseini Amin Javidanbardan Behnaz Sadat Alizadeh Salim 《Preparative biochemistry & biotechnology》2013,43(8):683-692
AbstractThe costly media, inconsistent ligand density, ligand leakage, and possible destabilization of recombinant hepatitis B surface antigen (rHBsAg) particles are main drawbacks of using immunoaffinity chromatography (IAF) in the large-scale downstream processing. In this study, we aimed to use an efficient large-scale purification system as an alternative purification method for immunoaffinity chromatography. For this purpose, we suggested integrating non-affinity chromatographic methods of hydrophobic interaction chromatography (HIC) and size-exclusion chromatography (SEC) for cost-effective purification of rHBsAg expressed in P. pastoris. The optimization of such process is not trivial and straightforward since diverse molecular characteristics of expressed rHBsAg in each type of host cell cause different interactions in non-affinity chromatography processes. The working buffer composition and chromatography parameters are the most influential factors in hydrophobic interaction chromatography. The best result for lab-scale HIC was achieved by using ammonium sulfate buffer in 10% of saturation concentration in pH 7.0 with Butyl-S Sepharose 6 Fast Flow medium and with subsequent Tween-100 and urea elution. In this process, the recovery, purity, and total yield were about 84%, 82%, and 69%, respectively. By scaling-up the HIC and integrating it with Sephacryl S-400?SEC, we obtained highly pure, i.e.,?>?90%, rHBsAg virus-like particles (VLP). 相似文献
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Habibzadeh Seyyedeh Zahra Salehzadeh Ali Moradi-Shoeili Zeinab Shandiz Seyed Ataollah Sadat 《Molecular biology reports》2020,47(3):1637-1647
Molecular Biology Reports - Gastric cancer is one of the common types of cancer around the world which has few therapeutic options. Nitrogen heterocyclic derivatives such as thiazoles are used as... 相似文献
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Fereshteh Khorrami Oruj Valizadegan Maryam Forouzan Asmar Soleymanzade 《Archives Of Phytopathology And Plant Protection》2013,46(13-14):685-695
AbstractRed flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) is a prominent pest of stored products particularly cereal flour. Since resistance of this pest to common chemical insecticides is well documented, we were examined the synergistic/antagonistic interaction between Satureja hortensis L., Trachyspermum ammi L., Ziziphora tenuior L., Cuminum cyminum L. and Foeniculum vulgare Miller essential oils, ethanolic extracts and powders with Diatomaceous earth (DE) against T. castaneum adults under laboratory conditions at 27 ± 1 °C, 65 ± 5% RH and continuous darkness. We assayed repellency of ethanolic extracts and essential oils of mentioned plants on the pest. Results showed that DE had high toxicity to the pest. Plant essential oils and ethanolic extracts (except ziziphora) synergized the performance of DE. Nevertheless, plant powders elicited antagonistic effects (except ziziphora that exhibited synergistic effect). The most repellent EO and extract was cumin which exhibited mean repellency value on adult insect equivalent to 92.58 and 51.47%, respectively. 相似文献
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Kobra Omidfar Fatemeh Sadat Amjad Zanjani Arghavan Golbaz Hagh Maedeh Darziani Azizi Seyed Javad Rasouli Susan Kashanian 《Molecular biology reports》2013,40(12):6737-6745
Epidermal growth factor receptor (EGFR) is deemed to be one of the main molecular targets for diagnosis and treatment of cancer. It has been identified that EGFR involves in pathogenesis of some forms of human cancers. Monoclonal antibodies targeting EGFR could control the tumor cell growth, proliferation, and apoptosis by suppressing the signal transduction pathways. Nanobodies can be regarded as the smallest intact antigen binding fragments, derived from heavy chain-only antibodies existing in camelids. Here, we describe the identification of an EGFR-specific nanobody, referred to as OA-cb6, obtained from immunized camel with a cell line expressing high levels of EGFR. Utilizing flow cytometry (FACS) and blotting methods, we demonstrated that OA-cb6 nanobody binds specifically to EGFR expressing on the surface of A431 cells. In addition, OA-cb6 nanobody potently causes the inhibition of EGFR over expression, cell growth and proliferation. The antibody fragments can probably be regarded as worthwhile binding block for further rational design of anti-cancer therapy. 相似文献
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Reza Ebrahimzadeh-Vesal Seyed kianush Hosseini Fereshteh Rezakhanlu Pupak Derakhshandeh-Peykar 《Reports of Biochemistry & Molecular Biology》2013,2(1):52-55
Holt-Oram syndrome (HOS) is a developmental disorder inherited in an autosomal-dominant pattern. Affected organs are the heart and forelimbs with upper extremity skeletal defects and congenital heart malformation. In this study we present three cases of HOS in the same family. In one of these three individuals we detected a transition of C to T (CTG-GTT, V205V) in exon 7 of the TBX5 gene. This nucleotide change causes no amino acid change and potential pathologic effects remain unknown.Key Words: Holt-Oram syndrome, Congenital heart malformation, TBX5 gene 相似文献
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Zhongzhao Teng Umar Sadat Wenkai Wang Nasim S. Bahaei Shengyong Chen Victoria E. Young Martin J. Graves Jonathan H. Gillard 《PloS one》2013,8(4)