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Repeat-induced point mutation (RIP) in Neurospora results in inactivation of duplicated DNA sequences. RIP is thought to provide protection against foreign elements such as retrotransposons, only one of which has been found in N. crassa. To examine the role of RIP in nature, we have examined seven N. crassa strains, identified among 446 wild isolates scored for dominant suppression of RIP. The test system involved a small duplication that targets RIP to the easily scorable gene erg-3. We previously showed that RIP in a small duplication is suppressed if another, larger duplication is present in the cross, as expected if the large duplication competes for the RIP machinery. In two of the strains, RIP suppression was associated with a barren phenotype--a characteristic of Neurospora duplications that is thought to result in part from a gene-silencing process called meiotic silencing by unpaired DNA (MSUD). A suppressor of MSUD (Sad-1) was shown not to prevent known large duplications from impairing RIP. Single-gene duplications also can be barren but are too short to suppress RIP. RIP suppression in strains that were not barren showed inheritance that was either simple Mendelian or complex. Adding copies of the LINE-like retrotransposon Tad did not affect RIP efficiency. 相似文献
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A convenient assay to score repeat-induced point mutation (RIP) inNeurospora employs theerg-3 locus as a mutagenesis target. Using this assay we screened 132 wild-isolatedNeurospora crassa strains for ability to dominantly suppress RIP. RIP was exceptionally inefficient in crosses with the wild isolates Sugartown
(P0854) and Adiopodoume-7 (P4305), thereby suggesting the presence of dominant RIP suppressors in these strains. In other
experiments, we found no evidence for dominant RIP suppression by theSpore killer haplotypesSk-2 andSk-3. 相似文献
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Patrick Yusuf Lee Alice Raha Oishik Pillai Kavya Gupta Shubham Sethi Sonika Mukeshimana Felicite Gerard Lothaire Moghal Mohammad U. Saleh Sohag N. Smith Susan F. Morrell Mary J. Moss James 《Sleep and biological rhythms》2017,15(3):217-225
Sleep and Biological Rhythms - Sleep deprivation is common among university students, and has been associated with poor academic performance and physical dysfunction. However, current literature... 相似文献
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