The effect of diode laser and light emitting diode on the bacterial contamination of semen medium for artificial insemination.

Animal artificial insemination (AI) suffers from bacterial contamination of semen media which results in decreased success of the process of artificial insemination. It is difficult to treat the semen extender medium to reduce the bacterial growth in the presence of semen using the conventional techniques of bacterial inhibition. In the present work, a new optical method developed for bacterial growth inhibition in semen containing extender medium using diode laser (DL) and commercial cheap light emitting diode (LED) is presented. Certain wavelengths and exposure times suitable for the process of artificial insemination are found to be optimum at reducing bacterial growth with a minimum significant effect on the semen motility and viability.     

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Plasma LH was measured by radioimmunoassay after the administration of gonadotropin releasing analogue (Gonadorelin, Hoechst) in five buffalo heifers in January and June. A longer time from injection to the LH peak and a lower LH peak height was found in June as compared with January. The difference between the two seasons in the area under the LH response curve, however, was not significant. These data suggest that the known lower ovarian activity during the summer season may be associated with a decrease in the level of pituitary activity.