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1.
    
Atrial fibrillation (AF) is a frequent arrhythmia in patients undergoing hemodialysis (HD). P wave duration (PWdu) and P wave dispersion (PWdi) have been shown to be predictors of emerging AF in different clinical conditions. We sought to study the impact of HD on PWdu, PWdi, and P wave amplitude in a cohort of patients undergoing HD. Seventeen patients (8 men, 31+/-10 years) were studied. Echocardiography parameters, the sum of the amplitude of P waves in all 12 ECG leads (SP), mean PWdu, and PWdi, along with a host of other parameters (body weight, heart rate, electrolytes and hemoglobin/hematochrit) were measured 1/2h, before and after, HD. SP increased (11.8+/-3.9 vs 15.3+/-4.0 mm, p = 0.004), mean PWdu remained stable (82.7+/-11.1 vs 81.6+/-10.5 ms, p = 0.606), PWdi decreased (51.7+/-19.1 vs 41.7+/-19.1 ms, p = 0.03), and left atrial dimension decreased (37.96+/-3.90 vs 30.62+/-3.38 mm, p = 0.0001), after HD. The change in PWdi correlated with fluid removed by HD (r = -0.55, p = 0.022). Re-measurements of P-wave parameters in a random group of 11 of the 17 patients revealed augmented SP (p = 0.01), and stable mean PWdu (p = 0.36), and PWdi (p = 0.31), after HD. Fluid removed by HD leads to an increase in SP, a stable mean PWdu, and decrease (or stability on re-measurement in a subgroup of patients) in PWdi. Stability of PWdu may be due to the effects of augmentation of the P-wave amplitude and the reduction of the left atrial volume, cancelling each other. Variability of PWdi may stem from the occasional impossibility to measure PWdu (or measure it correctly) in minute P-waves in certain ECG leads, which in turn profoundly affects the PWdi.  相似文献   
2.
An integrated multi-disciplinary study of nine North African lakes (CASSARINA) aims to establish ecological baselines and to explore responses to 20th century human impacts on their ecosystems. Water chemistry measurements (1997–1998) demonstrate a wide range from dilute oligotrophic to calcareous freshwaters and from mildly brackish to hypersaline lagoons. The biota are consequently highly diverse. Aquatic ecosystem responses to environmental stress over the last 100–200 years in all nine lakes are summarised by detrended correspondence analysis (DCA) of plant and animal macrofossil, zooplankton, diatom, and pollen data from short sediment cores. DCA proved to be a powerful tool for summarising multi-proxy sediment records and ecosystem dynamics. Compositional changes measured by the DCAs have been very large and rapid, often over a few decades; as great as climate-controlled late-glacial changes over 2000 years and larger than most Holocene (11000 years) changes. These results emphasise the strength of human impact on the lakes and the surprisingly great resilience and dynamism of their ecosystems. The DCA summaries for the most recent decades indicate ecosystem disequilibrium in all the lakes, implying that their future stability is uncertain and that large or damaging changes may soon occur if the stresses are maintained. Thresholds have recently been passed in 3 lakes. During the project, Merja Bokka (Morocco) was drained and cultivated. The unique acid Megene Chitane (Tunisia) is in danger of drying up permanently due to water extraction. Freshwater diversion from Garaet El Ichkeul (Tunisia) has dramatically altered its wildlife habitat, as reed-marshes were replaced by salt-marsh and bare mud within 20 years. In contrast, the ecosystems of the Delta lakes (Egypt) have responded dramatically to the year-round inflow of fresh irrigation water controlled by Nile dams and the rise in the freshwater table due to inadequate drainage in the flat delta. The Project has demonstrated remarkably rapid responses by the lakes to environmental stresses. In particular, it highlights the threats to wetland-lake ecosystems in North Africa if uncontrolled exploitation continues.  相似文献   
3.
Within the framework of the international research project MELMARINA, seasonal dynamics of plankton communities in three North African coastal lagoons (Merja Zerga, Ghar El Melh, and Lake Manzala) were investigated. The sampling period extended from July 2003 to September 2004 with the aim of evaluating hydrological and other influences on the structure, composition and space-time development of these communities in each lagoon. Phytoplankton in Merja Zerga showed a quasi-permanent predominance of marine diatoms in the open sea station and in the marine inlet channel. Dinoflagellates were abundant in summer and early autumn in the marine inlet and extended into the central lagoon station. In Ghar El Melh, marine species (especially diatoms and dinoflagellates) dominated despite occasional winter inflows of freshwater. In Lake Manzala, freshwater species generally predominated and the planktonic communities were comparatively very diverse. Chlorophyceae contributed 39% of the total species recorded and diatoms and cyanophyceans were also common; the Dinophyceae, Euglenophyceae, Chrysophyceae and Cryptophyceae less so. Zooplankton communities in both Ghar El Melh and Merja Zerga were dominated by marine copepods. Rotifera, Copepoda, Ostracoda, and Cladocera were recorded in both lagoons as were meroplanktonic larvae of Polychaeta, Cirripedia, Mysidacea and Gastropoda and free living nematodes. Ghar El Melh was the more productive of these two lagoons with spring and early summer being the productive seasons. Zooplankton communities in Lake Manzala were generally dominated by rotifers and highest zooplankton abundances occurred in April (2003). Sampling stations near the marine inlets showed the highest diversity and the zooplankton communities showed considerable spatial variation within this large lagoon. The three lagoons represent very different water bodies contrasted strongly in terms of tidal effects and freshwater availability. Yet, there are some similarities in ecosystem structure. Space-time development of the plankton communities was similar especially in Merja Zerga and Ghar El Melh. Species abundances and specific diversities indicated that seasonal changes in salinity and nutrient concentrations were the main influential factors. Lake Manzala was the most productive lagoon and all the three sites supported toxic algal species. Relatively low plankton biomass in Merja Zerga and Ghar El Melh probably resulted from a combination of factors including highly episodic nutrient inputs, light suppression (by turbidity) and nutrient competition with benthic algae. Water quality variables were largely driven by the hydrological regime specific to each lagoon. Nutrient enrichment and, particularly for Lake Manzala, sea level rise threaten the sustainability of the planktonic ecosystems in all three lagoons. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons  相似文献   
4.
Increasing prices and demand for biofuel and cooking oil from importer countries have caused a remarkable expansion of oil palm plantations in Indonesia. In this paper, we attempt to monitor the expansion of oil palm plantations on peat land and in tropical forests. We measure the GHG emissions from the land conversion activities at provincial scale. Using Landsat images from three different periods (1990s, 2000s and 2012), we classified LULC of the Riau Province, which is the largest oil palm producing region in Indonesia. A hybrid method of integration, generated by combining automatic processing and manual analysis, yields the best results. We found that the tropical rainforest cover decreased from ∼63% in the 1990s to ∼37% in the 2000s. By 2012, the remaining tropical rainforest cover was only ∼22%. From the 1990s to the 2000s, conversion of forests and peat lands was the primary source of emissions, total CO2 emitted to the atmosphere was estimated at ∼26.6 million tCO2.y-1, with 40.62% and 59.38% of the emissions from conversion of peat lands and forests, respectively. Between 2000 and 2012, the total CO2 emitted to the atmosphere was estimated at ∼5.2 million tCO2. y-1, with 69.94% and 27.62% of the emissions from converted peat lands and converted forests, respectively. The results show that in the Riau Province, the oil palm industry boomed in the period from 1990 to 2000, with transformation of tropical forest and peat land as the primary source of emissions. The decrease of CO2 emissions in the period from 2000 to 2012 is possibly due to the enforcement of a moratorium on deforestation.  相似文献   
5.
Age and growth characteristics of the thin-lipped Grey Mullet (Liza ramada) were investigated in three North African wetland lakes: Merja Zerga (Morocco), Garâat Ichkeul (Tunisia) and Edku Lake (Egypt). Age structure of the mullet populations was very similar in all three study lakes. Small differences in growth were indicated, especially for the Moroccan population, where growth tended to be slower than for the other two populations. The fastest growth was observed in the Edku population while the best condition was observed in the Ichkeul population. Compared with some European populations, the sampled North African populations have faster growth and better condition factors.  相似文献   
6.
Palaeolimnological studies were carried out on sediment cores from nine North African wetland lakes. The lakes represented a variety of habitats ranging from freshwater to mixohaline conditions and with and without direct connections to the sea. Sediment cores were examined for records of recent environmental change during the 20th century period. Faunal remains analysed in the sediments included those of Cladocera, Ostracoda, Chironomidae, Mollusca and, at the sites with marine connections, Foraminifera. Cyprideis torosa littoralis was the most common ostracod, occurring in sediments from the seven sites linked with the sea and also in brackish water Sidi Bou Rhaba. In acid Megene Chitane ostracods were scarce, being represented by a single species (Cypria ophtalmica). Candona neglecta completely disappeared at two sites (Sidi Bou Rhaba and Bokka) during the 20th century. Of the Cladocera, Chydorus sphaericus was common in the moderately mixohaline sites and but Heterocypris salina occurred only where marine salinities were occasionally achieved (in Zerga, Ichkeul and Korba). Microinvertebrate assemblages in the Nile Delta lake cores and to a less extent in Zerga showed a clear response indicative of more fresher conditions occurring during the latter part of the 20th century. However the freshening trend began prior to construction of the Aswan High Dam (mid 1960s).With the exception of the most saline site (Korba), microinvertebrate communiuties at all the CASSARINA sites have experienced major disturbances during the 20th century. Changes in freshwater availability associated with increased human usage of water resources is perceived as a major factor regulating the abundance and occurrence of aquatic microinvertebrate species at these sites  相似文献   
7.
All nine wetland lakes in the CASSARINA North African suite of sample sites have been disturbed strongly by human activity during the 20th century. Dated lake sediment core were used to provide evidence of the extent of recent environmental change at each site. Sedimentary diatoms at seven sites were useful for inferring salinity change trends during the last century. At two sites preservation problems severely degraded the sedimentary diatom record. Sediment core integrity was otherwise established.Lithostratigraphic measurements indicated some site specific changes in soil erosion and sediment composition but, for the Egyptian Delta lakes, no physical signal synchronous with Aswan High Dam construction was found. Sedimentary diatom assemblages were generally site characteristic and halophilous taxa were common. At two sites planktonic diatoms indicated some recent eutrophication but generally the assemblages were more indicative of salinity changes. Diatom-inferred salinity trends for the seven sites typically indicated that reductions in water salinity occurred sometime during the early or mid 20th century.Rather than climate, hydrological modification of water resources is implicated as the primary driver of salinity changes during most of the 20th century. In the western North African region these modifications were mainly local land drainage and water diversion programmes to alleviate winter flooding and/or promote summer water availability. In the Delta region, the Nile has been intensively exploited since antiquity and intensively so from the late 19th century to release more fresh water for agriculture. Here, diatom records indicate that freshening began well before the Aswan High Dam but salinity fluctuations have tended to diminish during the latter part of the 20th century. A small reversal in the water freshening trend in the 1980/90s was possibly a response to land subsidence/sea-level change or to reduced freshwater supply.Freshwater supply to the sites is generally diminishing as former freshwater surpluses switch to deficit. One site (Merja Bokka, Morocco) became completely dry in 1998 as agriculture encroached and Megene Chitane, the only acid lake in Tunisia, is currently affected by excessive inflow abstraction. At the beginning of the 21st century, eight of the nine CASSARINA sites persist as viable but modified aquatic ecosystems. They nevertheless continue to support valuable aquatic biodiversity, especially in the Delta sites and in Chitane. The modern diatom communities are clearly tolerant of considerable environmental change but the remaining sites are increasingly threatened by major hydrological disturbance. Base-line floristic data for the late 20th century are given but continuous biomonitoring combined with effective management is needed urgently to help conserve North Africa's diminishing natural wetland lake resources.  相似文献   
8.
All pathogenesis and death associated with Plasmodium falciparum malaria is due to parasite-infected erythrocytes. Invasion of erythrocytes by P. falciparum merozoites requires specific interactions between host receptors and parasite ligands that are localized in apical organelles called micronemes. Here, we identify cAMP as a key regulator that triggers the timely secretion of microneme proteins enabling receptor-engagement and invasion. We demonstrate that exposure of merozoites to a low K+ environment, typical of blood plasma, activates a bicarbonate-sensitive cytoplasmic adenylyl cyclase to raise cytosolic cAMP levels and activate protein kinase A, which regulates microneme secretion. We also show that cAMP regulates merozoite cytosolic Ca2+ levels via induction of an Epac pathway and demonstrate that increases in both cAMP and Ca2+ are essential to trigger microneme secretion. Our identification of the different elements in cAMP-dependent signaling pathways that regulate microneme secretion during invasion provides novel targets to inhibit blood stage parasite growth and prevent malaria.  相似文献   
9.
This article describes the use of satellite imagery for identifying key environmental characteristics within three North African coastal lagoons (Merja Zerga, Morocco; Ghar El Melh, Tunisia and Lake Manzala, Egypt) and for detecting the major environmental changes within these environments. A combination of Landsat MSS, Landsat TM, Landsat ETM+ and ASTER imagery was acquired for the three sites for a period covering the last three decades (1972–2004). Following geometric correction and enhancement, the interpretation of the most recent image acquired for each of the three lagoons provides important insights into their current conditions. For Merja Zerga, these include the distribution of the largest channels which drain extensive inter-tidal mudflats and the two major depositional features associated with sources of freshwater. The distribution of marginal aquatic vegetation is highlighted as is the intensive use of the surrounding landscape for agriculture. Intensive agriculture around Ghar El Melh is also indicated. The influence of the Mejerda River, which was diverted away from the lagoon over 100 years ago, is shown to persist as a residual area of deltaic deposits in shallow water that has been eroded over time. Coastal processes including the direction of the alongshore sediment transport and the influence of engineering work associated with port construction can also be recognised. Within Lake Manzala, vegetated islands divide the lake into a series of sub-basins which can be clearly distinguished. The large influence of human activities within this lake can be identified and include reclamation for agriculture and the conversion of parts of the lake bed for fish farms. The historical images available for the three lagoons provide important insights into decadal scale changes, which have been greatest at Lake Manzala. Since the early 1970s large parts of the lake, in particular in the southwest where the shoreline has migrated northwards, have been reclaimed. Major engineering works, such as the El Salam Canal and road embankments, are shown to have resulted in significant lake change. The distribution of emergent vegetation within the lake has also changed. Classification of images for this lake into open water, vegetation and land enables the quantification of these changes. Between 1973 and 2003, the lake declined in area by approximately 50%. Changes at Merja Zerga over the last three decades include reconfiguration of the marine outlet and the expansion of the internal delta at the end of the Nador Canal. The images of this site clearly demonstrate the intensification of agriculture around the lagoon. The most marked changes evident within the images of Ghar El Melh concern the sand bars that separate the lagoon from the sea. Geomorphological processes operating within the coastal zone have resulted in the straightening of the bars with central sections migrating out towards the sea. Remote sensing is established as a promising application for detecting the quantitative surface cover changes in coastal lagoons and their near landscapes. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons  相似文献   
10.
During plant sexual reproduction, pollen germination and tube growth require development under tight spatial and temporal control for the proper delivery of the sperm cells to the ovules. Pollen tubes are fast growing tip-polarized cells able to perceive multiple guiding signals emitted by the female organ. Adhesion of pollen tubes via cell wall molecules may be part of the battery of signals. In order to study these processes, we investigated the cell wall characteristics of in vitro-grown Arabidopsis (Arabidopsis thaliana) pollen tubes using a combination of immunocytochemical and biochemical techniques. Results showed a well-defined localization of cell wall epitopes. Low esterified homogalacturonan epitopes were found mostly in the pollen tube wall back from the tip. Xyloglucan and arabinan from rhamnogalacturonan I epitopes were detected along the entire tube within the two wall layers and the outer wall layer, respectively. In contrast, highly esterified homogalacturonan and arabinogalactan protein epitopes were found associated predominantly with the tip region. Chemical analysis of the pollen tube cell wall revealed an important content of arabinosyl residues (43%) originating mostly from (1→5)-α-l-arabinan, the side chains of rhamnogalacturonan I. Finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of endo-glucanase-sensitive xyloglucan showed mass spectra with two dominant oligosaccharides (XLXG/XXLG and XXFG), both being mono O-acetylated, and accounting for over 68% of the total ion signals. These findings demonstrate that the Arabidopsis pollen tube wall has its own characteristics compared with other cell types in the Arabidopsis sporophyte. These structural features are discussed in terms of pollen tube cell wall biosynthesis and growth dynamics.Fertilization of flowering plants requires the delivery of the two sperm cells, carried by a fast growing tip-polarized pollen tube, to the egg cell. In plants with dry stigma and solid style such as Arabidopsis (Arabidopsis thaliana), this process begins with the deposition and specific adhesion of the pollen grains on the stigmatic tissue, subsequent hydration of the pollen grains, and germination of pollen tubes (Palanivelu and Preuss, 2000). Pollen tubes invade the papillae cell wall of the stigma, enter the short style, and grow through the apoplast of the specialized transmitting tract (TT) that is filled with a nutrient-rich extracellular matrix (Kandasamy et al., 1994; Lennon et al., 1998). During this invasive growth, pollen tubes are guided to the ovules via signals that need to pass through the cell wall to reach their membrane-associated or intracellular targets (Lord and Russell, 2002; Kim et al., 2003; Boavida et al., 2005; McCormick and Yang, 2005; Johnson and Lord, 2006). In plant species with wet stigma and hollow style such as lily (Lilium longiflorum), adhesion between the pollen tube wall and the TT epidermis extracellular matrix is important for the growth of the pollen tubes toward the ovules (Mollet et al., 2000, 2007; Park et al., 2000; Chae et al., 2007). In addition to being the interface between the tube cells and the surroundings (female sporophyte or culture medium), the pollen tube wall also controls the cell shape, protects the generative cells, and allows resistance against turgor pressure (Geitmann and Steer, 2006; Geitmann, 2010).Most of our knowledge on cell wall polymers of higher plants comes from investigations on vegetative organs in which cells have diffuse growth. The cell wall is mainly composed of polysaccharides (cellulose, hemicellulose, pectin, and occasionally callose, depending on the tissue) and proteoglycans (e.g. extensin and arabinogalactan proteins [AGPs]) forming a complex network with processing enzymes.Pectins are complex wall macromolecules with uncertain supramolecular organization (Vincken et al., 2003) consisting of homogalacturonan (HG) that can be methylesterified and acetylesterified, rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II), and xylogalacturonan (Carpita and McCann, 2000). HG is a polymer of repeated units of (1→4)-α-d-GalUA that can be cross-linked with calcium upon block-wise action of pectin methylesterases (PMEs) on methylesterified HG (Micheli, 2001). RG-II has the same homopolymer backbone as HG but is substituted with four different oligosaccharides composed of unusual sugars, such as apiose, aceric acid, and 3-deoxy-d-manno-2-octulosonic acid, of unknown function (for review, see Caffall and Mohnen, 2009). RG-I consists of the repeating disaccharide (1→4)-α-d-GalUA-(1→2)-α-l-Rha, with a wide variety of side chains attached to the rhamnosyl residues, ranging from monomers to large oligosaccharides such as (1→4)-β-d-galactan, (1→5)-α-l-arabinan, and/or type I arabinogalactan (Caffall and Mohnen, 2009).Xyloglucan (XyG) is the major hemicellulosic polysaccharide of the primary wall of flowering plants. Classic XyG consists of a (1→4)-β-d-glucan backbone substituted with Xyl, Gal-Xyl, or Fuc-Gal-Xyl motifs, which correspond, according to the one-letter code proposed by Fry et al. (1993), to X, L, and F, respectively, G being the unsubstituted glucosyl residue of the glucan backbone. The main XyG fragments released after endo-glucanase treatment of the cell wall from wild-type Arabidopsis vegetative organs are generally XXXG, XXLG/XLXG, XXFG, and XLFG (Zablackis et al., 1995; Lerouxel et al., 2002; Nguema-Ona et al., 2006; Obel et al., 2009). In addition, O-acetylation of XyG can occur, most generally on the galactosyl residues, but its biological function is unknown (Cavalier et al., 2008). In the primary wall, XyG interacts with cellulose microfibrils via hydrogen bonds and participates in the control of cell expansion (Cosgrove, 1999).AGPs and extensin belong to the Hyp-rich glycoproteins superfamily with very high levels of type II arabinogalactan glycosylation (Nothnagel, 1997; Showalter, 2001). These proteoglycans have been implicated in many aspects of plant development, including cell expansion, cell signaling and communication, embryogenesis, wound response, and pollen tube guidance (Wu et al., 1995; Nothnagel, 1997; Seifert and Roberts, 2007; Driouich and Baskin, 2008).Despite the importance of pollen tubes for the delivery of the sperm cells to the egg, little is known about the underlying molecular mechanisms that regulate the mechanical interaction of pollen tubes with female floral tissues. There are very scarce data concerning the different components of the pollen tube cell wall. Past approaches to characterize the pollen tube cell wall are limited to a few plant genera, including Camellia (Nakamura and Suzuki, 1981), Lilium (Jauh and Lord, 1996; Mollet et al., 2002), Nicotiana (Rae et al.,1985; Li et al., 1995; Ferguson et al., 1998; Qin et al., 2007), Pinus (Derksen et al., 1999), and Zea (Rubinstein et al., 1995), and are mostly based on immunocytochemistry. These studies revealed that, depending on the species, the pollen tube cell wall contains epitopes that are found in the polymers described above, including HGs with varying levels of methylesterification, AGPs, extensin-like proteins, and low amounts of cellulose. Unlike most other plant cells, callose, a (1→3)-β-glucan, is predominant and is deposited in the wall back from the tip. Moreover, it is deposited at regular intervals to form callose plugs that maintain the tube cell in the apical expanding region of the tube and separate the viable from the degenerating region of the tube (for review, see Geitmann and Steer, 2006). Only a few reports have investigated the pollen tube of the model plant Arabidopsis. They have focused either on in vivo-grown or on in vitro-grown pollen tubes using monoclonal antibodies (MAbs) directed against a subset of cell wall epitopes present in HG, XyG, and AGPs (Lennon and Lord, 2000; Freshour et al., 2003; Pereira et al., 2006), but quantitative chemical analyses are lacking. This lack of information is most likely due to the fact that substantial amounts of pollen tube material are needed for chemical analysis, and a reproducible and efficient method for liquid culture of Arabidopsis pollen tubes had not been established until recently (Boavida and McCormick, 2007; Bou Daher et al., 2009).Here, we report the composition and localization of different cell wall polymers of in vitro-grown wild-type Arabidopsis pollen tubes based on biochemical analyses coupled to immunocytochemical investigations both at light and transmission electron microscopy (TEM) levels using recently developed MAbs. Our results show distinct patterns of labeling (tip, whole tube, and shank of the tube) depending on the recognized epitope. The most striking observations are (1) the abundance of (1→5)-α-l-arabinan in the tube wall (greater than 40 mol % of Ara), mostly localized, with LM6 and LM13, in the outer wall layer of the tube and (2) an atypical XyG matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profile with over 68% of the oligosaccharide fragments being O-acetylated.  相似文献   
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