排序方式: 共有124条查询结果,搜索用时 62 毫秒
1.
2.
Faten Talmoudi Olfa Kilani Wiem Ayed Nizar Ben Halim Fethi Mellouli Lamia Torjmane Lamia Aissaoui Yosra Ben Youssef Lobna Kammoun Tarek Ben Othmane Mohamed Bejaoui Neila Ben Romdhane Moez Elloumi Sondes Hadiji Sofiene Hentati Imene Chemkhi Nabila Abidli Helmi Guermani Sonia Abdelhak Ahlem Amouri 《Comptes rendus biologies》2013,336(1):29-33
Fanconi anemia (FA) is a recessive chromosomal instability syndrome that is clinically characterized by multiple symptoms. Chromosome breakage hypersensitivity to alkylating agents is the gold standard test for FA diagnosis. In this study, we provide a detailed laboratory protocol for accurate assessment of FA diagnosis based on mitomycin C (MMC) test. Induced chromosomal breakage study was successful in 171 out of 205 aplastic anemia (AA) patients. According to the sensitivity of MMC at 50 ng/ml, 38 patients (22.22%) were diagnosed as affected and 132 patients (77.17%) as unaffected. Somatic mosaicism was suspected in an 11-year-old patient with a FA phenotype. Twenty-six siblings of FA patients were also evaluated and five of them (19.23%) were diagnosed as FA. From this study, a standard protocol for diagnosis of FA was developed. It is routinely used as a diagnostic test of FA in Tunisia. 相似文献
3.
Ksontini Hamid Kachouri Faten El Abed Soumya Ibnsouda Koraichi Saad Meftah Hasna Latrache Hassan Hamdi Moktar 《Microbiology》2013,82(1):22-28
The initial microorganism adhesion on substrate is an important step for the biofilm formation. The surface properties of the stainless steel and B. cereus were characterized by the sessile drop technique. Moreover, the physicochemical properties of surface adhesion and the impact of bio adhesion to the stainless steel were determined at different time of contact (2, 4, 7, 9 and 24 h). The results showed that the strain was hydrophilic (Giwi = 3.37 mJ/m2), whereas the substratum has hydrophobic character (Giwi = ?57.6 mJ/m2). Stainless steel surface presents a weak electron-donor character (γ? = 4.1 mJ/m2) conversely to B. cereus that presents an important parameter (γ? = 31.6 mJ/m2). The bio adhesion was investigated at different time of contact. The data analysis after 2 h, confirmed the adhesion of B. cereus with an amount of 10 cfu/cm2 which increased to 1.2104 cfu/cm2 after 24 h. Interestingly, despite the difference of hydropohbicity, the interaction between B. cereus and substratum was favored by the thermodynamic aspect of adhesion (ΔGadhesion < 0). Interestingly, the study of the effect of B. cereus adhesion on the stainless steel has revealed that, the substratum becomes hydrophilic (θ° = 41.3, ΔGiwi = 39.6 mJ/m2) and highly electron donor (Γ? = 52.9 mJ/m2) after 2 h of bio adhesion. 相似文献
4.
Faten A. Hamdy Samir Borham Gad Ali Aldosari 《Archives Of Phytopathology And Plant Protection》2019,52(3-4):277-287
A greenhouse experiment was carried-out to evaluate the effect of three rates of salinity as abiotic stress on okra plants (Abelmoschus esculentus) infected with the root-knot nematode (Meloidogyne incognita) as biotic stress. Plant lengths and weights were significantly (p?≤?0.05) reduced except root weight and there was a positive correlation between increasing the salinity concentration from 0.1 to 0.3% and increasing the rate of reduction in plant criteria. The number of J2 in soil, galls, and eggmasses were decreased linked to increased salinity rate as compared to nematode control treatment. However, peroxidase and catalase activities were significantly reduced linked to increasing the salinity concentration from 0.1 to 0.3%. There was no significant difference between total phenols at all treatments. Meanwhile, there was no significant improvement in N, P, and K contents whereas photosynthetic pigments (a, b) and carotene were significantly (p?≤?0.05) reduced by nematode infection and increasing the salinity rate from 0.1 to 0.3%. 相似文献
5.
Sixteen Arab lactating mares belonging to Al-Zahraa Arab Horse Stud underwent two ultrasound examinations at 3 weeks interval starting from the day of demonstration of foaling heat. In addition, daily blood samples were collected from parturition until after exhibiting first postpartum estrus (day 11) with daily observation of estrous signs. Both leptin and estradiol hormones were assayed. Mean day of foaling heat was 8.9 ± 0.9 day. Most mares came in foaling heat during days 9 and 10 had high conception rate compared to those who came in estrus earlier or later. Estradiol levels were high after day of foaling then decrease after expression of foaling heat. But leptin levels increase from day 8 to day 10 compared to other days before and after the first ovulation. A significant positive correlation was found between estradiol and leptin (r = 0.58, p < 0.025). The positive correlation between leptin and estradiol led us to suggest that leptin hormone plays an important role in ovulation of the first postpartum estrus in mares. 相似文献
6.
Caulobacter crescentus synthesizes an S-layer-editing metalloprotease possessing a domain sharing sequence similarity with its paracrystalline S-layer protein 下载免费PDF全文
Umelo-Njaka E Bingle WH Borchani F Le KD Awram P Blake T Nomellini JF Smit J 《Journal of bacteriology》2002,184(10):2709-2718
Strains of Caulobacter crescentus elaborate an S-layer, a two-dimensional protein latticework which covers the cell surface. The S-layer protein (RsaA) is secreted by a type I mechanism (relying on a C-terminal signal) and is unusual among type I secreted proteins because high levels of protein are produced continuously. In efforts to adapt the S-layer for display of foreign peptides and proteins, we noted a proteolytic activity that affected S-layer monomers with foreign inserts. The cleavage was precise, resulting in fragments with an unambiguous N-terminal sequence. We developed an assay to screen for loss of this activity (i.e., presentation of foreign peptides without degradation), using transposon and traditional mutagenesis. A metalloprotease gene designated sap (S-layer-associated protease) was identified which could complement the protease-negative mutants. The N-terminal half of Sap possessed significant similarity to other type I secreted proteases (e.g., alkaline protease of Pseudomonas aeruginosa), including the characteristic RTX repeat sequences, but the C-terminal half which normally includes the type I secretion signal exhibited no such similarity. Instead, there was a region of significant similarity to the N-terminal region of RsaA. We hypothesize that Sap evolved by combining the catalytic portion of a type I secreted protease with an S-layer-like protein, perhaps to associate with nascent S-layer monomers to "scan" for modifications. 相似文献
7.
8.
Nina Desai Anastasia Alex Faten AbdelHafez Anthony Calabro James Goldfarb Aaron Fleischman Tommaso Falcone 《Reproductive biology and endocrinology : RB&E》2010,8(1):119
In vitro ovarian follicle culture is a new frontier in assisted reproductive technology with tremendous potential, especially
for fertility preservation. Folliculogenesis within the ovary is a complex process requiring interaction between somatic cell
components and the oocyte. Conventional two-dimensional culture on tissue culture substrata impedes spherical growth and preservation
of the spatial arrangements between oocyte and surrounding granulosa cells. Granulosa cell attachment and migration can leave
the oocyte naked and unable to complete the maturation process. Recognition of the importance of spatial arrangements between
cells has spurred research in to three-dimensional culture system. Such systems may be vital when dealing with human primordial
follicles that may require as long as three months in culture. In the present work we review pertinent aspects of in vitro
follicle maturation, with an emphasis on tissue-engineering solutions for maintaining the follicular unit during the culture
interval. We focus primarily on presenting the various 3-dimensional culture systems that have been applied for in vitro maturation
of follicle:oocyte complexes. We also try to present an overview of outcomes with various biomaterials and animal models and
also the limitations of the existing systems. 相似文献
9.
Friedlein G El Hage F Vergnon I Richon C Saulnier P Lécluse Y Caignard A Boumsell L Bismuth G Chouaib S Mami-Chouaib F 《Journal of immunology (Baltimore, Md. : 1950)》2007,178(11):6821-6827
We previously characterized several tumor-specific T cell clones from PBL and tumor-infiltrating lymphocytes of a lung cancer patient with identical TCR rearrangements and similar lytic potential, but with different antitumor response. A role of the TCR inhibitory molecule CD5 to impair reactivity of peripheral T cells against the tumor was found to be involved in this process. In this report, we demonstrate that CD5 also controls the susceptibility of specific T cells to activation-induced cell death (AICD) triggered by the tumor. Using a panel of tumor-infiltrating lymphocytes and PBL-derived clones expressing different levels of CD5, our results indicate that T lymphocyte AICD in response to the cognate tumor is inversely proportional to the surface expression level of CD5. They also suggest a direct involvement of CD5 in this process, as revealed by an increase in tumor-mediated T lymphocyte AICD following neutralization of the molecule with specific mAb. Mechanistically, our data indicate that down-regulation of FasL expression and subsequent inhibition of caspase-8 activation are involved in CD5-induced T cell survival. These results provide evidence for a role of CD5 in the fate of peripheral tumor-specific T cells and further suggest its contribution to regulate the extension of CTL response against tumor. 相似文献
10.
Mala Isrie Martin Breuss Guoling Tian Andi?Harley Hansen Francesca Cristofoli Jasmin Morandell Zachari?A. Kupchinsky Alejandro Sifrim Celia?Maria Rodriguez-Rodriguez Elena?Porta Dapena Kurston Doonanco Norma Leonard Faten Tinsa Stéphanie Moortgat Hakan Ulucan Erkan Koparir Ender Karaca Nicholas Katsanis Valeria Marton Joris?Robert Vermeesch Erica?E. Davis Nicholas?J. Cowan David?Anthony Keays Hilde Van?Esch 《American journal of human genetics》2015,97(6):790-800
Circumferential skin creases Kunze type (CSC-KT) is a specific congenital entity with an unknown genetic cause. The disease phenotype comprises characteristic circumferential skin creases accompanied by intellectual disability, a cleft palate, short stature, and dysmorphic features. Here, we report that mutations in either MAPRE2 or TUBB underlie the genetic origin of this syndrome. MAPRE2 encodes a member of the microtubule end-binding family of proteins that bind to the guanosine triphosphate cap at growing microtubule plus ends, and TUBB encodes a β-tubulin isotype that is expressed abundantly in the developing brain. Functional analyses of the TUBB mutants show multiple defects in the chaperone-dependent tubulin heterodimer folding and assembly pathway that leads to a compromised yield of native heterodimers. The TUBB mutations also have an impact on microtubule dynamics. For MAPRE2, we show that the mutations result in enhanced MAPRE2 binding to microtubules, implying an increased dwell time at microtubule plus ends. Further, in vivo analysis of MAPRE2 mutations in a zebrafish model of craniofacial development shows that the variants most likely perturb the patterning of branchial arches, either through excessive activity (under a recessive paradigm) or through haploinsufficiency (dominant de novo paradigm). Taken together, our data add CSC-KT to the growing list of tubulinopathies and highlight how multiple inheritance paradigms can affect dosage-sensitive biological systems so as to result in the same clinical defect. 相似文献