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1.
Muhammad Rizwan Ingrid Miller Fareeha Tasneem Josef Böhm Manfred Gemeiner Ebrahim Razzazi-Fazeli 《Mycotoxin Research》2010,26(3):171-180
Genome sequencing for many important fungi has begun during recent years; however, there is still some deficiency in proteome
profiling of aspergilli. To obtain a comprehensive overview of proteins and their expression, a proteomic approach based on
2D gel electrophoresis and MALDI-TOF/TOF mass spectrometry was used to investigate A. ochraceus. The cell walls of fungi are exceptionally resistant to destruction, therefore two lysis protocols were tested: (1) lysis
via manual grinding using liquid nitrogen, and (2) mechanical lysis via rapid agitation with glass beads using MagNalyser.
Mechanical grinding with mortar and pestle using liquid nitrogen was found to be a more efficient extraction method for our
purpose, resulting in extracts with higher protein content and a clear band pattern in SDS-PAGE. Two-dimensional electrophoresis
gave a complex spot pattern comprising proteins of a broad range of isoelectric points and molecular masses. The most abundant
spots were subjected to mass spectrometric analysis. We could identify 31 spots representing 26 proteins, most of them involved
in metabolic processes and response to stress. Seventeen spots were identified by de novo sequencing due to a lack of DNA
and protein database sequences of A. ochraceus. The proteins identified in our study have been reported for the first time in A. ochraceus and this represents the first proteomic approach with identification of major proteins, when the fungus was grown under submerged
culture. 相似文献
2.
Zhang Q Zulfiqar F Xiao X Riazuddin SA Ahmad Z Caruso R MacDonald I Sieving P Riazuddin S Hejtmancik JF 《Human genetics》2007,122(3-4):293-299
Mutation in the PROM1 gene previously has been identified in one family with retinal degeneration for which neither ERG recordings
nor detailed information about visual impairment is available. A large family with multiple individuals affected by retinal
degeneration was ascertained in the Punjab province of Pakistan. The visual acuity of all affected patients in the family
was severely compromised beginning in early childhood. The retinal disease in this family is a severe form of retinitis pigmentosa
(RP) accompanied by macular degeneration. Fundus changes advanced with age. Choriocapillaris atrophy and posterior RPE atrophy
were obvious allowing visualization of the large choroidal vessels in patients over 40 years of age. Rod and cone responses
on ERG recordings were extinguished in patient’s teens. A genome-wide scan mapped the disease to a 34.7 cM region of chromosome
4p14–p16 between D4S1599 and D4S405. A maximum lod score of 3.96 with D4S403 and D4S391 is seen at θ = 0. Sequence analysis
of PROM1 located in the linkage interval identified a c.1726C>T homozygous transition in exon 15: resulting in p.Gln576X in
the translated protein. This mutation is found in a homozygous state in all six affected individuals and was heterozygous
in five of the six unaffected family members examined. The mutation was not detected in 192 chromosomes of unrelated control
individuals of the same ethnicity and from the same region. This delineates the phenotypic characteristics of retinopathy
caused by mutations in PROM1.
Qingjiong Zhang, Fareeha Zulfiqar, Xueshan Xiao, Sheikh Riazuddin and J. Fielding Hejtmancik contributed equally. 相似文献
3.
Improving vanadium stress tolerance of watermelon by grafting onto bottle gourd and pumpkin rootstock 总被引:1,自引:0,他引:1
Muhammad Azher Nawaz Chen Chen Fareeha Shireen Zhuhua Zheng Yanyan Jiao Hamza Sohail Muhammad Afzal Muhammad Imtiaz Muhammad Amjad Ali Yuan Huang Zhilong Bie 《Plant Growth Regulation》2018,85(1):41-56
Vanadium (V) is a transition metal found in the Earth crust. V adversely affects plant growth and development. Besides several other management practices, grafting of scion cultivars onto appropriate rootstock provides a suitable solution. Grafting is an important agro-technical procedure utilized to enhance the capacity of plants to tolerate biotic and abiotic stresses. In this study, watermelon was grafted onto bottle gourd and pumpkin rootstock, and self-grafted watermelon plants were utilized as a control. V was applied at the rate of 50 mg/L under hydroponic conditions. The result showed that V application substantially reduces the growth of watermelon plants, however, grafting of watermelon onto bottle gourd and pumpkin rootstock improves V stress tolerance of watermelon by reducing the V concentration in leaf tissues, improving the relative chlorophyll content (SPAD index) and photosynthetic assimilation, up-regulating the expression of SOD (Cla008698, Cla0012125, Cla009820 and Cla001158), glutathione S-transferase (Cla013224) and glutathione peroxidase (Cla021039) genes in the leaves, and enhancing the activities of antioxidant enzymes (SOD, CAT). The scanning electron microscopy (SEM) of the root tips showed that minimal damage of roots was observed for pumpkin roots compared with the roots of watermelon and bottle gourd under V stress conditions. So far as we know, these results are the first evidence that grafting mitigates V stress in plants. 相似文献
4.
Ali Meissam Saleem Uzma Anwar Fareeha Imran Muhammad Nadeem Humaira Ahmad Bashir Ali Tahir Atta-ur-rehman Ismail Tariq 《Neurochemical research》2021,46(4):905-920
Neurochemical Research - Alzheimer's disease (AD) is age-dependent neurological disorder with progressive loss of cognition and memory. This multifactorial disease is characterized by... 相似文献
5.
Bushra Irum Shahid Y. Khan Muhammad Ali Haiba Kaul Firoz Kabir Bushra Rauf Fareeha Fatima Raheela Nadeem Arif O. Khan Saif Al Obaisi Muhammad Asif Naeem Idrees A. Nasir Shaheen N. Khan Tayyab Husnain Sheikh Riazuddin Javed Akram Allen O. Eghrari S. Amer Riazuddin 《PloS one》2016,11(11)
PurposeTo identify the molecular basis of non-syndromic autosomal recessive congenital cataracts (arCC) in a consanguineous family.MethodsAll family members participating in the study received a comprehensive ophthalmic examination to determine their ocular phenotype and contributed a blood sample, from which genomic DNA was extracted. Available medical records and interviews with the family were used to compile the medical history of the family. The symptomatic history of the individuals exhibiting cataracts was confirmed by slit-lamp biomicroscopy. A genome-wide linkage analysis was performed to localize the disease interval. The candidate gene, LIM2 (lens intrinsic membrane protein 2), was sequenced bi-directionally to identify the disease-causing mutation. The physical changes caused by the mutation were analyzed in silico through homology modeling, mutation and bioinformatic algorithms, and evolutionary conservation databases. The physiological importance of LIM2 to ocular development was assessed in vivo by real-time expression analysis of Lim2 in a mouse model.ResultsOphthalmic examination confirmed the diagnosis of nuclear cataracts in the affected members of the family; the inheritance pattern and cataract development in early infancy indicated arCC. Genome-wide linkage analysis localized the critical interval to chromosome 19q with a two-point logarithm of odds (LOD) score of 3.25. Bidirectional sequencing identified a novel missense mutation, c.233G>A (p.G78D) in LIM2. This mutation segregated with the disease phenotype and was absent in 192 ethnically matched control chromosomes. In silico analysis predicted lower hydropathicity and hydrophobicity but higher polarity of the mutant LIM2-encoded protein (MP19) compared to the wild-type. Moreover, these analyses predicted that the mutation would disrupt the secondary structure of a transmembrane domain of MP19. The expression of Lim2, which was detected in the mouse lens as early as embryonic day 15 (E15) increased after birth to a level that was sustained through the postnatal time points.ConclusionA novel missense mutation in LIM2 is responsible for autosomal recessive congenital cataracts. 相似文献
6.
Muhammad Ansar Regie Lyn P. Santos-Cortez Muhammad Arif Nadeem Saqib Fareeha Zulfiqar Kwanghyuk Lee Naeem Mahmood Ashraf Ehsan Ullah Xin Wang Sundus Sajid Falak Sher Khan Muhammad Amin-ud-Din Joshua D. Smith Jay Shendure Michael J. Bamshad Deborah A. Nickerson Abdul Hameed Saima Riazuddin Zubair M. Ahmed Wasim Ahmad Suzanne M. Leal University of Washington Center for Mendelian Genomics 《Human genetics》2015,134(9):941-950
7.
Despite the constantly increasing need for new antimicrobial agents, antibiotic drug discovery and development seem to have
greatly decelerated in recent years. Presented with the significant problem of advancing antimicrobial resistance, the global
scientific community has attempted to find alternative solutions; one of the most promising ones is the evaluation and use
of old antibiotic compounds. A number of old antibiotic compounds, such as aminoglycosides, chloramphenicol, and tetracycline,
are re-emerging as valuable alternatives for the treatment of difficult-to-treat infections. This study examined the in vitro
potency for biofilm formation of five isolates (Klebsiella sp., Pseudomonas aeruginosa, Achromobacter sp., Klebsiella pneumoniae, and Bacillus pumilis) and the effects of antibiotics on these biofilms. Furthermore the quantitative analysis of planktonic, loosely attached
cells, and their susceptibility to antibiotics was also determined. Twitching motility was observed to determine any effect
in the biofilm forming capability of the isolates. All the isolates tested were efficient biofilm-forming strains in the polypropylene
and borosilicate test tubes. Standard bacterial enumeration technique and CV staining produced equivalent results both in
biofilm and planktonic assays. The biofilm formation of all the strains was affected in the presence of tetracycline or chloramphenicol.
Highly significant decrease (P < 0.01) in biofilm formation was observed by treatment with chloramphenicol compared to tetracycline. In addition, the two
antibiotics also affected adversely the planktonic and loosely attached cells of all isolates. Thus, testing the effects of
older antibiotics on biofilms may supply useful information in addition to standard in vitro testing, particularly in diseases
where biofilm formation is involved in the pathogenesis. 相似文献
8.
Zhang Q Zulfiqar F Xiao X Riazuddin SA Ayyagari R Sabar F Caruso R Sieving PA Riazuddin S Hejtmancik JF 《Human genetics》2005,118(3-4):356-365
A severe form of autosomal recessive retinitis pigmentosa (arRP) was identified in a large Pakistani family ascertained in the Punjab province of Pakistan. All affected individuals in the family had night blindness in early childhood, early complete loss of useful vision, and typical RP fundus changes plus macular degeneration. After exclusion of known arRP loci, a genome-wide scan was performed using microsatellite markers at about 10 cM intervals and calculating two-point lod scores. PCR cycle dideoxynucleotide sequencing was used to sequence candidate genes inside the linked region for mutations. RP in this family shows linkage to markers in a 10.5 cM (8.9 Mbp) region of chromosome 1p13.3-p21.2 between D1S2896 and D1S457. D1S485 yields the highest lod score of 6.54 at theta=0. Sequencing the exons and intron-exon boundaries of five candidate genes and six ESTs in this region, OLFM3, GNAI3, LOC126987, FLJ25070, DKFZp586G0123, AV729694, BU662869, BU656110, BU171991, BQ953690, and CA397743, did not identify any causative mutations. This novel locus lies approximately 4.9 cM (7.1 Mbp) from ABCA4, which is excluded from the linked region. Identification and study of this gene may help to elucidate the phenotypic diversity of arRP mapping to this region. 相似文献
9.
Nazir Samra Anwar Fareeha Saleem Uzma Ahmad Bashir Raza Zohaib Sanawar Maham Rehman Artta ur Ismail Tariq 《Neurochemical research》2021,46(7):1814-1829
Neurochemical Research - Alzheimer’s disease (AD) is a progressive neurodegenerative disease associated with decline in memory and cognitive impairments. Phosphodiesterase IV (PDE4) protein,... 相似文献
10.
Parambi Della Grace Thomas Saleem Uzma Shah Muhammad Ajmal Anwar Fareeha Ahmad Bashir Manzar Amna Itzaz Aqsa Harilal Seetha Uddin Md Sahab Kim Hoon Mathew Bijo 《Neurochemical research》2020,45(11):2786-2799
Neurochemical Research - Parkinson’s disease (PD) is a neurodegenerative disorder of dopaminergic, noradrenergic, and serotonergic systems, in which dopamine, noradrenaline, and serotonin... 相似文献