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排序方式: 共有154条查询结果,搜索用时 15 毫秒
1.
N. Romero C. Marsac M. Fardeau M. Droste B. Schneyder B. Kadenbach 《Histochemistry and cell biology》1990,94(2):211-215
Summary The immunohistochemical reaction of monoclonal as well as polyclonal antibodies against cytochrome c oxidase (COX) subunits
with serial sections of normal human skeletal muscle was investigated. The stronger reactivity of polyclonal antibodies to
COX subunits II–III and VIIbc with type I as compared to type II fibres, correlated well with the higher histochemical reactivity
of NADH dehydrogenase, succinate dehydrogenase and cytochrome c oxidase in type I fibres. In contrast an almost exclusive
reaction of a monoclonal antibody against subunit IV with type I fibre and a preponderan reaction of a polyclonal antibody
against subunits Vab with type II fibres was obtained. Antibodies against subuntis I, Vb and VIc did not reveal a fibre-type-specific
reactivity. The data indicate in human muscle the occurrence of fibre type-specific isozymes of cytochrome c oxidase differing
in subunits IV and Va or Vb. 相似文献
2.
C Fardeau M Langlois D Ingrand C Asselot H Moret F Nugier 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》1992,314(10):443-449
During 7 serial passages of Herpes Simplex Virus (HSV1) in rabbit cornea treated with idoxuridine (IUdR) (P1 to P7), the emergence of resistance had been obtained from P3. The reversion towards IUdR sensitivity has been investigated from either viral population P3 or P6 by 6 serial passages in rabbit cornea treated by Vaseline (V1 to V6). From viral population P3, the reversion to IUdR sensitivity has been obtained at V4. In contrast, from viral population P6, the IUdR resistance was conserved from V1 to V6. In vitro, on Vero cells, the effective doses 50% (ED50) and 90% (ED90), determined by dye uptake assay and plaque reduction assay, confirmed the reversibility towards IUdR sensitivity obtained from P3 and the stability of IUdR resistance from P6. 相似文献
3.
Summary The optimum conditions for growth ofFrankia sp. HFPCcI3 isolated fromCasuarina cunninghamiana, were studied in batch culture using defined media. Maximum growth (doubling time was 24 h)_was achieved at 33°C and at pH 6.3 with pyruvate and NH
4
+
as the sole C and N sources, respectively. Removal of NH
4
+
from the culture medium resulted in vesicle differentiation which was paralleled by induction of acetylene reduction activity. Growth on atmospheric N2 was optimal with combined pyruvate and glucose as the carbon sources and displayed a doubling time of about 48 h. Comparisons in growth and N2-fixing activity ofFrankia strains grown in a variety of cultural conditions demonstrate the range of behavior among the strains. 相似文献
4.
C Delaporte B Dautreaux M Fardeau 《Biology of the cell / under the auspices of the European Cell Biology Organization》1986,57(1):17-22
Human muscle cells derived from satellite cells, maintained in standard tissue culture conditions, do not differentiate as rapidly or as completely as myoblasts from other species (chicken, rat, mouse). In an attempt to improve myogenesis, we studied the effects of modifying the culture media and of coculturing muscle with nerve cells, using myoblasts grown in standard culture media as the basis for comparison. Myogenesis was measured by fusion index, creatine kinase (CK) activity; acetylcholinesterase (AChE) activity (total and molecular forms); and the number of acetylcholine receptors (AChR). Modification of culture media accelerated fusion of myoblasts, but the cell density decreased and myotubes were unable to survive for long periods. In contrast, coculturing muscle with nerve cells increased both cell density and the number of myotubes. CK, AChE and AChR increased in the presence of defined media. In the nerve-muscle cocultures the increase was less marked. Manipulating culture conditions modified the molecular forms of AChE. Only a (4 + 6.5) S peak was present in control cultures, but a 10S peak appeared in defined media. The 16S form was detected only in nerve-muscle cocultures. This study shows that fusion of human myoblasts and differentiation of myotubes in tissue culture can be accelerated by removal of serum macromolecules. Further differentiation of myotubes was achieved only in the nerve-muscle cocultures. 相似文献
5.
Fertilizers labelled with 32P were used to measure amounts of phosphorus, Ps and PF, taken up by Lolium perenne from available soil P and from P fertilizer respectively, when applied at a rate of 66 mg P·(kg soil–1) in greenhouse experiments. The quantity Ps of phosphorus taken up from soil in the presence of P fertilizer was compared to the quantity Po taken up from soil without P fertilizer. The quantity (Ps–Po) is positive for low Po values, i.e. in soils poor in available phosphorus, but is negative for high Po values indicating that an input of P fertilizer can induce a decrease in the utilization of available soil phosphorus. Moreover, for a given soil, the quantity (Ps–Po) depends on the chemical form of the fertilizer. The standard method of evaluation of P fertilizer efficiency is based on the assumption that Ps=Po, but Ps can differ from Po. This result can explain the contradictory data published from field experiments about the efficiency of the various P fertilizers. 相似文献
6.
Evidence for heterogeneity in facioscapulohumeral muscular dystrophy (FSHD) 总被引:4,自引:0,他引:4 下载免费PDF全文
J. R. Gilbert J. M. Stajich S. Wall S. C. Carter H. Qiu J. M. Vance C. S. Stewart M. C. Speer J. Pufky L. H. Yamaoka M. Rozear F. Samson M. Fardeau A. D. Roses M. A. Pericak-Vance 《American journal of human genetics》1993,53(2):401-408
Facioscapulohumeral muscular dystrophy (FSHD) is a slowly progressive primary disease of muscle which is usually inherited as an autosomal dominant disorder. FSHD has been localized to the long arm of chromosome 4, specifically to the 4q3.5-qter region. Initially published linkage studies showed no evidence for heterogeneity in FSHD. In the present study we have examined individuals in seven FSHD families. Two-point lod scores show significant evidence for linkage for D4S163 (lod score 3.04 at recombination fraction .21) and D4S139 (lod score 3.84 at recombination fraction .20). D4S171 also gave a positive score (lod score 2.56 at recombination fraction .24). Significant evidence for heterogeneity was found for each of the three markers. Multipoint linkage analysis in this region resulted in a peak multipoint lod score of 6.47. The multipoint analysis supported the two-point studies with odds of 20:1 showing linkage and heterogeneity over linkage and homogeneity. Five of the seven families gave a posterior probability of >95% of being of the linked type, while two families appeared unlinked to this region of 4q (P < .01%). Individuals in the two unlinked families met the clinical criteria for the diagnosis of FSHD, including facial weakness, clavicular flattening, scapula winging, proximal muscle weakness, and myopathic changes on muscle biopsies without inflammatory or mitochondrial pathology. This study demonstrates genetic heterogeneity in FSHD and has important implications for both genetic counseling and the elucidation of the etiology of FSHD. 相似文献
7.
Thiosulfate Reduction, an Important Physiological Feature Shared by Members of the Order Thermotogales 总被引:4,自引:1,他引:3 下载免费PDF全文
G. Ravot B. Ollivier M. Magot B. Patel J. Crolet M. Fardeau J. Garcia 《Applied microbiology》1995,61(5):2053-2055
Several members of the order Thermotogales in the domain Bacteria, viz., Thermotoga neapolitana, Thermotoga maritima, Thermosipho africanus, Fervidobacterium islandicum, and Thermotoga strain SEBR 2665, an isolate from an oil well, reduced thiosulfate to sulfide. This reductive process enhanced cellular yields and growth rates of all the members but was more significant with the two hyperthermophiles T. neapolitana and T. maritima. This is the first report of such an occurrence in this group of thermophilic and hyperthermophilic anaerobic bacteria. The results suggest that thiosulfate reduction is important in the geochemical cycling of sulfur in anaerobic thermal environments such as the slightly acidic and neutral-pH volcanic hot springs and oil reservoirs. 相似文献
8.
Stefanie Possekel Anne Lombes Helene Ogier de Baulny Marie-Arnelle Cheval Michel Fardeau Bernhard Kadenbach Norma B. Romero 《Histochemistry and cell biology》1995,103(1):59-68
Despite the demonstration of a clear biochemical defect, the genetic alterations causing childhood forms of cytochromec oxidase (COX) deficiency remain unknown. The double genetic origin (nuclear and mitochondrial DNA), and the complexity of COX enzyme structure and regulation, indicate the need for genetic iinvestigations of the molecular structure of individual COX subunits. In the present study a new monoclonal antibody, which reacts exclusively with heart-type human COX subunit VIIa (VIIa-H), and other monoclonal antibodies against human COX subunits, were used in the immunohistochemical analysis of skeletal muscle from children with different forms of mitochondrial myopathy with COX deficiency. By immunohistochemical investigation a normal reaction was seenn with antibodies to COX subunits IV, Va+Vb, and VIa+VIc in all four cases, and in two cases with antibodies to COX VIIa-H and VIIa+VIIb. In muscle from a fatal infantile case with cardiac and skeletal muscle involvement, no immunohistochemical reaction was seen with the monoclonal antibody against the tissue-specific subunit VIIa-H. In muscle from an 11-year-old boy with exclusive muscular symptoms and signs, immunohistological reactions were absent with COX subunit VIIa-H and COX subunits VIIa+VIIb, and slightly decreased with COX subunit II, thus demonstrating a different molecular mechanism in each case. It is concluded that the molecular basis of COX deficiency in childhood may vary greatly between patients. 相似文献
9.
Summary A microcalorimetric study is proposed to follow the degradation of straw by a mixed bacterial culture. The effect of an alkali pretreatment of straw is described. 相似文献
10.