沿海防护林防海煞危害初探

通过后牧村海岸防护林带附近空气中主要盐离子飘尘的观测分析表明,林带对大气盐尘的吸附和截留作用,无林带地区平均沉降量为４．３μｇ．ｍ＾－３．１００ｍ＾－１,通过林带时为１８．４μｍ＾－３．１００ｍ＾－１。林带的吸队量与树种、枝叶形状有关,侧柏对Ｃｌ＾－和Ｎａ＾＋的吸附量分别为６３３０和３３５０ｍｇ．ｋｇ＾－１,而黑松为１５７０和９５０ｍｇ．ｋｇ＾－１,空气中盐尘受天气条件特别是风速的影响,阴天,风速     

Enhancing Specific-Antibody Production to the ragB Vaccine with GITRL That Expand Tfh,IFN-γ+ T Cells and Attenuates Porphyromonas gingivalis Infection in Mice

The outer membrane protein RagB is one of the major virulence factors of the periodontal pathogen Porphyromonas gingivalis (P. gingivalis). In order to induce protective immune response against P. gingivalis infection, an mGITRL gene-linked ragB DNA vaccine (pIRES-ragB-mGITRL ) was constructed. Six-week-old female BALB/c mice were immunized with pIRES-ragB-mGITRL through intramuscular injection and then challenged by subcutaneous injection in the abdomen with P. gingivalis. RagB-specific antibody-forming cells were evaluated by an Enzyme-linked immunosorbent spot, and specific antibody was determined by enzyme-linked immunosorbent assay. In addition, the frequencies of Tfh and IFN-γ⁺ T cells in spleen were measured using flow cytometer, and the levels of IL-21 and IFN-γ mRNA or proteins were detected by real time RT-PCR or ELISA. The data showed that the mGITRL-linked ragB DNA vaccine induced higher levels of RagB-specific IgG in serum and RagB-specific antibody-forming cells in spleen. The frequencies of Tfh and IFN-γ⁺ T cells were obviously expanded in mice immunized by pIRES-ragB-mGITRL compared with other groups (pIRES or pIRES-ragB ). The levels of Tfh and IFN-γ⁺ T cells associated cytokines were also significantly increased in pIRES-ragB-mGITRL group. Therefore, the mice immunized with ragB plus mGITRL showed the stronger resistant to P. gingivalis infection and a significant reduction of the lesion size caused by P. gingivalis infection comparing with other groups. Taken together, our findings demonstrated that intramuscular injection of DNA vaccine ragB together with mGITRL induced protective immune response dramatically by increasing Tfh and IFN-γ⁺ T cells and antibody production to P. gingivalis.     

土壤镉污染与作物

通过土壤镉污染对作物影响的盆栽模拟试验,以揭示重金属镉在土壤—植物系统中的转移、分布规律及其对植物生长、发育的影响。试验证明不同化合形态的镉施入土壤(砂壤质褐土,pH值8.2),水稻对镉吸收的多寡依次为CdCl2>CdSO4>CdO>CdS>CdCO3。白菜的镉吸收表现为CdSO4>CdCl2>CdO>CdCO3。土壤的不同镉浓度(施加CdCl2,以纯镉计)对作物影响的试验结果表明,可食部位达到食品污染标准(谷物含镉量0.4ppm、蔬菜0.2ppm为暂定标准)时的土壤镉污染临界值分别为：小麦、莴苣、白菜<1ppm,茄子、萝卜<2.5ppm,番茄、菜豆<20ppm。土壤因子的处理影响镉的活动性;降低土壤pH值,水稻的镉吸收增加。增施有机肥、ZnSO4、S、CaO、CaSO4可降低糙米含镉量13.4%一30%。白菜的镉吸收,由于增施有机肥、FeSO4、Fe2O3、CaO或S而降低菜叶含镉量28%一61%。以Cd,Zn比1:100或1:200施入土壤,叶内含镉量分别下降61%和76.4%,但白菜产量减少61%和76%。     

薏苡糠壳的化学成分及其种子萌发活性研究

为了解薏苡(Coix lachryma-jobi)糠壳的化学成分,利用多种柱色谱技术对其乙醇提取物乙酸乙酯萃取部位进行分离,经波谱数据分析鉴定了15个化合物,分别为香豆酸(1)、香豆酸甲酯(2)、2-羟乙基-香豆酸酯(3)、咖啡酸甲酯(4)、阿魏酸甲酯(5)、(E)-3-(4-甲氧基苯基)丙烯酸(6)、2,3-二羟基-...     

SGT1 regulates Akt signaling by promoting beta-TrCP-dependent PHLPP1 degradation in gastric cancer cells

SGT1 (suppressor of G2 allele of Skp1) plays a role in various cellular processes including kinetochore assembly and protein ubiquitination by interacting with Skp1, a component of SCF E3 ligase complex. However, the function of SGT1 in cancer is largely unknown. Here, we showed that SGT1 was over-expressed in gastric cancer tissues and silencing of SGT1 by siRNAs significantly inhibited the growth and colony formation of gastric cancer cells. We further showed that SGT1 could regulate Akt signaling pathway by modulating Akt ser473 phosphorylation status. Moreover, we found that SGT1 was able to regulate the stability of PHLPP1, which is the direct phosphatase for Akt ser473 phosphorylation. Immunoprecipitation assay revealed that SGT1 could enhance the binding between PHLPP1 and beta-TrCP which has been documented to be able to target PHLPP1 for destruction. Decreased PHLPP1 in SGT1 over-expressed gastric cancer cells failed to dephosphorylate Akt and resulted in increased Akt ser473 phosphorylation and amplified downstream Akt signaling. Thus, our data revealed a previously uncovered role of SGT1 in gastric cancer development, and suggested that SGT1 could be a promising anti-cancer target to against gastric cancer.     

土壤中镉、铅、锌及其相互作用对作物的影响

通过作物盆栽模拟试验(砂壤质褐土、pH值8.2)揭示：土壤中分别施入镉(CdCl2)、铅[Pb(CH3COO)2]或锌(ZnSO4)其影响表现为,植物各器官镉的含量超过对照植物的数倍至500倍。土壤镉浓度<5ppm和<10ppm分别造成某些蔬菜和水稻的污染。铅主要积累在植物根部,土壤铅污染对作物的影响较小。锌主要积累在植物叶片和根部,对水稻产生生长抑制的土壤锌浓度临界值不大于200ppm,此浓度对旱作无影响。土壤中同时施入镉和铅,植物对镉的吸收增加。而土壤中镉的增加却减少了植物体内铅的含量。土壤中由于镉、锌或铅、锌相互作用的结果,水稻对它们的吸收都有增加。在旱地土壤锌浓度的增高,降低了植物对镉、铅的吸收。镉、铅、锌同时施入土壤由于相互作用的结果,除锌之外,植物对镉、铅的吸收有明显下降。评价土壤重金属污染,不仅要看它们的含量及其存在形态,而且要分析它们之间的相互作用(促进或拮抗)特点。     

贵州万山汞矿地区的植物及植物积累汞的研究

本文分析了矿区地表异常地段的土壤汞水平和植物群落,提出了自然条件下划分野生植物含汞水平标准,并研究了它们积累汞的规律。在大气含汞量极高的情况下,强富汞植物,弱富汞植物和中富汞植物都相应增加体内的汞积累。野菊花,大叶胡枝子,牛筋树可以作为大气汞污染物的净化植物。矿区农田土壤含汞量较高,农作物可食部位的含汞量都超标,菜蔬类尤为严重。在植物群落汞积累与环境因子关系的探讨中,认为草本植物和部分灌木的汞积累与下层土壤有机质和坡向关系密切,木本植物老枝含汞量受下层土壤含汞量的控制,也受土壤干湿度的影响。     

Four Novel Resistance Integron Gene-Cassette Occurrences in Bacterial Isolates from Zhenjiang, China

Integrons, which are widely distributed among bacteria and are strongly associated with resistance, are specialized genetic elements that are capable of capturing, integrating, and mobilizing gene cassette. In this work, we investigated classes 1, 2, and 3 integrons associated integrases genes in 365 bacteria isolates, amplified and analyzed the structure of class 1 integron, detected 8 resistant gene cassettes [dfr17, aadA5, aadA1, aadA2, dhfrI, aadB, aac(6′)-II, and pse-I], and found four novel gene-cassette arrays. We also found that commensal bacteria in the common microenvironment had the same integron gene cassette, which provided direct evidence that integron was an important horizontal transmission element.     

Identification of a six-gene prognostic signature for oral squamous cell carcinoma

Oral squamous cell carcinoma (OSCC) is one of the most common types of malignancies worldwide, and its morbidity and mortality have increased in the near term. Consequently, the purpose of the present study was to identify the notable differentially expressed genes (DEGs) involved in their pathogenesis to obtain new biomarkers or potential therapeutic targets for OSCC. The gene expression profiles of the microarray datasets GSE85195, GSE23558, and GSE10121 were obtained from the Gene Expression Omnibus (GEO) database. After screening the DEGs in each GEO dataset, 249 DEGs in OSCC tissues were obtained. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology pathway enrichment analysis was employed to explore the biological functions and pathways of the above DEGs. A protein–protein interaction network was constructed to obtain a central gene. The corresponding total survival information was analyzed in patients with oral cancer from The Cancer Genome Atlas (TCGA). A total of six candidate genes (CXCL10, OAS2, IFIT1, CCL5, LRRK2, and PLAUR) closely related to the survival rate of patients with oral cancer were identified, and expression verification and overall survival analysis of six genes were performed based on TCGA database. Time-dependent receiver operating characteristic curve analysis yields predictive accuracy of the patient's overall survival. At the same time, the six genes were further verified by quantitative real-time polymerase chain reaction using samples obtained from the patients recruited to the present study. In conclusion, the present study identified the prognostic signature of six genes in OSCC for the first time via comprehensive bioinformatics analysis, which could become potential prognostic markers for OCSS and may provide potential therapeutic targets for tumors.     

凤眼莲等水生植物对重金属污水监测和净化作用的研究

本文研究了凤眼莲、芦苇、蒲草、杏菜、黑藻等高等水生植物对含砷、汞、镉污水的监测作用和净化能力以及温度、pH值等因子对植物的影响。初步认为凤眼莲是含砷污水的监测植物,同时又是含砷、汞,镉污水的净化植物。芦苇,蒲草是抗污能力较强的植物,并能吸收积累污水中的砷、汞、镉。水生植物对砷、汞、镉的忍耐力大小是因其植物生活型不同而异,一般为挺水植物>飘浮、浮叶植物>沉水植物。积累量为沉水植物>飘浮、浮叶植物>挺水植物。植物对重金属元素的吸收积累受温度和pH值等生态因子的制约。