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1.
Falqueto A Sessa PA Ferreira AL Vieira VP Santos CB Varejão JB Cupolillo E Porrozzi R Carvalho-Paes LE Grimaldi Júnior G 《Memórias do Instituto Oswaldo Cruz》2003,98(8):1003-1010
Between 1985 and 2000, epidemiological surveys of the American tegumentary leishmaniasis (ATL) were carried out in several rural and urban communities in Espírito Santo, Brazil. A total of 100 stocks of Leishmania (comprising isolates from both human and canine hosts with ATL) were identified by two methods of molecular characterization, using specific monoclonal antibodies and multilocus enzyme electrophoresis. Parasite isolates from 19 municipalities were found to belong to the same zymodeme and serodeme type as of the Leishmania (Viannia) braziliensis reference strain. In contrast, our genotyping studies have shown intra-specific variation among these parasites (comparisons of the variability of the internal transcribed spacers between the small and large subunits of the rRNA genes of the 22 stocks studied revealed at least 11 genotypes). Two main clusters of L. (V.) braziliensis genotypes were observed, representing parasites collected from different endemic regions in the state, where transmission reflects distinct eco-epidemiological features. Infection with this pathogen was associated with the characteristic disease forms, but neither the clinical outcome nor the response to treatment could be related to the genetic polymorphism of the isolates, as defined by using the proposed methodology. 相似文献
2.
This paper describes sand flies similar to Nyssomyia intermedia (Lutz & Neiva) with variations in the number of spines at the gonostyle and tests the hypothesis whether these specimens belong or not to N. intermedia species. Using Principal Component Analysis and Neighbour Joining, the measurements of 15 structures of the phlebotomine with variations in number of spines were compared with measurements of 30 sand flies of N. intermedia species. Both analyses didn't cluster the specimens with variation in spines number in one group and provided evidence to the hypothesis that those sand flies belong to N. intermedia. 相似文献
3.
Sand fly fauna (Diptera: Psychodidae) from the Goytacazes National Forest and surrounding areas of southeastern Brazil
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Thieres Marassati das Virgens Helder Ricas Rezende Israel de Souza Pinto Aloísio Falqueto 《Journal of vector ecology》2015,40(1):28-35
Most studies of the sand fly fauna in southeastern Brazil are conducted in the peridomiciliary environment of leishmaniasis endemic regions. Therefore, to increase the knowledge about diversity and richness of sand fly conservation areas, we describe here the sand fly fauna from the National Forest of Goytacazes (NFG), state of Espírito Santo, Brazil, and its surroundings areas. We also used sand fly fauna records from eight conservations units within the state of Espírito Santo to understand the similarity and relationships among them. The sand flies were simultaneously collected from June, 2008 to May, 2009 in two different environments: a preserved environment represented by the NFG and a modified environment represented by a peridomicile. To establish the similarity among the conservation units, we used a method very similar to parsimony analysis of endemism. We collected 2,466 sand fly specimens belonging to 13 species. Pressatia choti and Nyssomyia intermedia were the most abundant sand fly species. Ny. intermedia is a known vector of Leishmania braziliensis and epidemiological surveillance must be conducted in the area. We discuss aspects regarding the diversity of sand flies as well as the risk of transmission of Leishmania parasites in the area. We also provide for the first time a hypothesis of similarity relationships among conservation units within the state of Espírito Santo. 相似文献
4.
dos Santos CB Ferreira AL Leite GR Ferreira GE Rodrigues AA Falqueto A 《Memórias do Instituto Oswaldo Cruz》2005,100(5):471-473
In Brazil, the colonization of human dwellings by triatomines occurs in areas with native vegetation of the caatinga or cerrado types. In areas of Atlantic forest such as in the Brazilian state of Espírito Santo, there are no species adapted to live in human habitations. The few autochthonous cases of Chagas disease encountered in Espírito Santo have been attributed to adult specimens of Triatoma vitticeps that invade houses from forest remnants. In recent years, the entomology unit of the Espírito Santo State Health Secretariat has recorded nymphs infected with flagellates similar to Trypanosoma cruzi in rural localities. Entomological surveys were carried out in the residences and outbuildings in which the insects were found, and serological examinations for Chagas disease performed on the inhabitants. Four colonies were found, all associated with nests of opossums (Didelphis aurita), 111 specimens of T. vitticeps, and 159 eggs being collected. All the triatomines presented flagellates in their frass. Mice inoculated with the faeces presented trypomastigotes in the circulating blood and groups of amastigotes in the cardiac muscle fibres. Serological tests performed on the inhabitants were negative for T. cruzi. Even with the intense devastation of the forest in Espírito Santo, there are no indications of change in the sylvatic habits of T. vitticeps. Colonies of this insect associated with opossum nests would indicate an expansion of the sylvatic environment into the peridomicile. 相似文献
5.
João Paulo Rodrigues Martins Luiz Carlos de Almeida Rodrigues Lorenzo Toscano Conde Andreia Barcelos Passos Lima Gontijo Antelmo Ralph Falqueto 《Plant biosystems》2020,154(1):87-99
AbstractUnder in vitro culture conditions, plants may present physiological and anatomical disorders, which can interfere negatively after ex vitro transfer. The aim of this investigation was to analyze the impacts of natural ventilation and sucrose supply on the anatomy and physiology of Vriesea imperialis. Plants previously grown in vitro were transferred to culture medium containing 0, 15, 30 or 45?g L?1 sucrose. Three different culture container sealing systems were tested: lids with a green filter (81.35 gas exchanges per day), yellow filter (13.09 gas exchanges per day) or lids with a yellow filter covered with three layers of transparent polyvinylchloride (PVC) film (blocking fluent gas exchange). Sucrose concentrations influenced thickness, lignin and suberin deposition of exodermis cell wall. The modifications verified in leaves, such as higher density of stomata and trichome scales, showed that sucrose can induce osmotic stress in the plants. Photomixotrophic conditions, using containers with intermediate rate of gas exchange (yellow filter) and with 15–30?g L?1 sucrose, produced an improvement in the growth traits and did not induce anatomical and physiological disturbances. 相似文献
6.
Israel de Souza Pinto Bruna Dias das Chagas Andressa Alencastre Fuzari Rodrigues Adelson Luiz Ferreira Helder Ricas Rezende Rafaela Vieira Bruno Aloisio Falqueto José Dilermando Andrade-Filho Eunice Aparecida Bianchi Galati Paloma Helena Fernandes Shimabukuro Reginaldo Pe?anha Brazil Alexandre Afranio Peixoto 《PloS one》2015,10(10)
DNA barcoding has been an effective tool for species identification in several animal groups. Here, we used DNA barcoding to discriminate between 47 morphologically distinct species of Brazilian sand flies. DNA barcodes correctly identified approximately 90% of the sampled taxa (42 morphologically distinct species) using clustering based on neighbor-joining distance, of which four species showed comparatively higher maximum values of divergence (range 4.23–19.04%), indicating cryptic diversity. The DNA barcodes also corroborated the resurrection of two species within the shannoni complex and provided an efficient tool to differentiate between morphologically indistinguishable females of closely related species. Taken together, our results validate the effectiveness of DNA barcoding for species identification and the discovery of cryptic diversity in sand flies from Brazil. 相似文献
7.
J. P. R. Martins E. R. Santos L. C. A. Rodrigues A. B. P. L. Gontijo A. R. Falqueto 《Biologia Plantarum》2018,62(4):793-800
Cytokinins (CKs) are often used during the in vitro cultivation of plant species. However, it is not clear how CKs, such as 6-benzylaminopurine (BAP), affect photosystem PS) II functionality and leaf anatomy over a long period of in vitro plant propagation. The aim of this study was to analyze the residual effects of BAP on the photosynthetic performance and leaf anatomy of Aechmea blanchetiana after 120 d without exposure to CKs. Aechmea blanchetiana plants previously grown in vitro were transferred to Murashige and Skoog (MS) culture media containing 0, 5, 10, 15, or 20 μM BAP. After 60 d on the MS medium with BAP, explants were subcultivated twice on the MS medium without growth regulators, first in a stationary liquid medium for 60 d and then in a solidified medium with 6 g dm-3 agar for 60 d. Leaf anatomy, pigment content, and chlorophyll a fluorescence were assessed for plants from each treatment after 120 d on the CK-free medium. Stomatal density presented a negative linear correlation with BAP concentration. Pigment content decreased in plants subjected to previous BAP exposure. An increase in absorbed energy flux per reaction center (ABS/RC) and a sharp decrease in energy transport flux (ETo/RC) followed by an increase in energy dissipation flux (DIo/RC) also occurred. Furthermore, maximum quantum yield (FV/FM) decreased as a function of BAP concentration. Thus, the use of BAP during in vitro propagation of A. blanchetiana induced long-term physiological defects. 相似文献
8.
Effect of deltamethrin spraying on the sandfly populations in a focus of American cutaneous leishmaniasis. 总被引:1,自引:0,他引:1
A L Falc?o A R Falc?o C T Pinto C M Gontijo A Falqueto 《Memórias do Instituto Oswaldo Cruz》1991,86(4):399-404
The effect of deltamethrin of the sandfly population in a focus of American Cutaneous Leishmaniasis in Viana, Espírito Santo State is described. The phlebotomine population density was determined inside and outside houses during a one year period in a treated and untreated area. The results showed a significant reduction in the number of sandflies inside houses in the treated area compared with both the untreated area and the same area before spraying. Despite having a residual action for 12 months after spraying the insecticide was ineffective outside houses. It is concluded therefore that deltamethrin is an efficient insecticide for reducing the number of phlebotomine sandflies inside houses. 相似文献
9.
Martins João Paulo Rodrigues Vasconcelos Leandro Lopes de Braga Priscila da Conceição de Souza Rossini Franciele Pereira Conde Lorenzo Toscano Rodrigues Luiz Carlos de Almeida Falqueto Antelmo Ralph Gontijo Andreia Barcelos Passos Lima 《Plant Cell, Tissue and Organ Culture》2020,143(2):303-318
Plant Cell, Tissue and Organ Culture (PCTOC) - Copper (Cu) is an essential metal and both its deficiency and excess has negative effects on the growth and physiology of plants. Some plant species... 相似文献
10.
Rossini Franciele Pereira Martins João Paulo Rodrigues Moreira Samuel Werner Conde Lorenzo Toscano Clairvil Evens Braga Priscila da Conceição de Souza Falqueto Antelmo Ralph Gontijo Andreia Barcelos Passos Lima 《Plant Cell, Tissue and Organ Culture》2022,150(1):223-236
Plant Cell, Tissue and Organ Culture (PCTOC) - Despite having the ability to bioaccumulate trace elements such as cadmium (Cd), many species also present morphophysiological disorders that can... 相似文献