全文获取类型
收费全文 | 58篇 |
免费 | 5篇 |
专业分类
63篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 5篇 |
2017年 | 1篇 |
2015年 | 1篇 |
2014年 | 1篇 |
2013年 | 2篇 |
2012年 | 7篇 |
2011年 | 2篇 |
2010年 | 1篇 |
2009年 | 1篇 |
2008年 | 1篇 |
2007年 | 4篇 |
2005年 | 3篇 |
2003年 | 1篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1994年 | 2篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1979年 | 2篇 |
1977年 | 1篇 |
1970年 | 1篇 |
1967年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有63条查询结果,搜索用时 0 毫秒
1.
Cloning of the hsp70 (dnaK) genes from Rhizobium meliloti and Pseudomonas cepacia: phylogenetic analyses of mitochondrial origin based on a highly conserved protein sequence. 总被引:2,自引:1,他引:2 下载免费PDF全文
The genes for hsp70 (or dnaK) have been cloned and sequenced from Rhizobium meliloti and Pseudomonas cepacia, two bacterial species belonging to the alpha- and beta-subdivisions of gram-negative proteobacteria, respectively. On the basis of global alignment of HSP70 proteins, several sequence signatures have been identified that are distinctive of mitochondrial homologs and gram-negative proteobacteria on the one hand and the chloroplasts and cyanobacteria on the other. Detailed phylogenetic analyses of HSP70 sequences from various eubacteria and eukaryotic organellar and cytosolic homologs support the inference regarding the origin of mitochondria from a member of the alpha-proteobacteria and of chloroplasts from cyanobacteria. The analysis presented here also suggests a monophyletic origin of the mitochondrial homologs. 相似文献
2.
The Evolutionary History of the Genus Acanthamoeba and the Identification of Eight New 18S rRNA Gene Sequence Types 总被引:6,自引:0,他引:6
Diane R. Stothard Jill M. Schroeder-Diedrich Mohammad H. Awwad Rebecca J. Gast Dolena R. Ledee Salvador Rodriguez-Zaragoza Chantal L. Dean Paul A. Fuerst Thomas J. Byers 《The Journal of eukaryotic microbiology》1998,45(1):45-54
ABSTRACT The 18S rRNA gene ( Rns ) phylogeny of Acanthamoeba is being investigated as a basis for improvements in the nomenclature and taxonomy of the genus. We previously analyzed Rns sequences from 18 isolates from morphological groups 2 and 3 and found that they fell into four distinct evolutionary lineages we called sequence types T1-T4. Here, we analyzed sequences from 53 isolates representing 16 species and including 35 new strains. Eight additional lineages (sequence types T5-T12) were identified. Four of the 12 sequence types included strains from more than one nominal species. Thus, sequence types could be equated with species in some cases or with complexes of closely related species in others. The largest complex, sequence type T4, which contained six closely related nominal species, included 24 of 25 keratitis isolates. Rns sequence variation was insufficient for full phylogenetic resolution of branching orders within this complex, but the mixing of species observed at terminal nodes confirmed that traditional classification of isolates has been inconsistent. One solution to this problem would be to equate sequence types and single species. Alternatively, additional molecular information will be required to reliably differentiate species within the complexes. Three sequence types of morphological group 1 species represented the earliest divergence in the history of the genus and, based on their genetic distinctiveness, are candidates for reclassification as one or more novel genera. 相似文献
3.
4.
5.
Najmabadi H Motazacker MM Garshasbi M Kahrizi K Tzschach A Chen W Behjati F Hadavi V Nieh SE Abedini SS Vazifehmand R Firouzabadi SG Jamali P Falah M Seifati SM Grüters A Lenzner S Jensen LR Rüschendorf F Kuss AW Ropers HH 《Human genetics》2007,121(1):43-48
Autosomal recessive gene defects are arguably the most important, but least studied genetic causes of severe cognitive dysfunction.
Homozygosity mapping in 78 consanguineous Iranian families with nonsyndromic autosomal recessive mental retardation (NS-ARMR)
has enabled us to determine the chromosomal localization of at least 8 novel gene loci for this condition. Our data suggest
that in the Iranian population NS-ARMR is very heterogeneous, and they argue against the existence of frequent gene defects
that account for more than a few percent of the cases.
Mohammad Mahdi Motazacker and Masoud Garshasbi have contributed equally to this work. 相似文献
6.
Zainuddin Zainal Zahari Tarmizi Reza Chee Yap Keng Sarkawi Nur Nabila Ahmad Hafandi Salleh Annas Tahir Nur Diyana Mohamad Baiee Falah Che-Amat Azlan Fitri Wan-Nor 《Primates; journal of primatology》2022,63(4):377-386
Primates - Bornean orangutan is a critically endangered non-human primate; however, the threat of extinction is not merely from poaching and habitat loss. Orangutan survival is also threatened by... 相似文献
7.
Awwad Abdoh Radwan 《Bioinformation》2015,11(2):63-66
cGMP-binding cGMP-specific PDE, PDE5 plays a key role in the hydrolysis of cyclic guanidine monophosphate. Because cGMP
mediates vascular functions, a PDE5 inhibitor that elevates cGMP level is an attractive means for vasodilatation and treatment of
erectile dysfunction. In this paper we report the elucidation of the common pharmacophore hypothesis of different classes of PDE5
inhibitors. Using LigandScout program, pharmacophore modelling studies were performed on prior reported potent PDE5
inhibitors with a variety of scaffolds in order to identify one common set of critical chemical features of these PDE5 inhibitors 1-52.
The best pharmacophore model, model-1, characterized by four chemical features: one aromatic ring, one hydrophobe, one
hydrogen acceptors and one hydrogen donor. Using Dock6 program, docking studies were performed in order to investigate the
mode of binding of these compounds. The molecular docking study allowed confirming the preferential binding mode of different
classes of PDE5 inhibitors inside the active site. The obtained binding mode was as same as that of vardenafil, X-ray ligand with
different orientation with varied PDE5 inhibitors׳ scaffold. 相似文献
8.
Mohammed Rafi Shaik Rabbani Syed Syed Farooq Adil Mufsir Kuniyil Mujeeb Khan Mohammed S. Alqahtani Jilani P. Shaik Mohammed Rafiq H. Siddiqui Abdulrahman Al-Warthan Mohammed A.F. Sharaf Abdelatty Abdelgawad Emad Mahrous Awwad 《Saudi Journal of Biological Sciences》2021,28(2):1196-1202
Due to their inexpensive and eco-friendly nature, and existence of manganese in various oxidation states and their natural abundance have attained significant attention for the formation of Mn3O4 nanoparticles (Mn3O4 NPs). Herein, we report the preparation of Mn3O4 nanoparticles using manganese nitrate as a precursor material by utilization of a precipitation technique. The as-prepared Mn3O4 nanoparticles (Mn3O4 NPs) were characterized by using X-ray powder diffraction (XRD), UV–Visible spectroscopy (UV–Vis), High-Resolution Transmission electron microscopy (HRTEM), Field emission scanning electron microscopy (FESEM), Thermal gravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FT-IR). The antimicrobial properties of the as-synthesized Mn3O4 nanoparticles were investigated against numerous bacterial and fungal strains including S. aureus, E. coli, B. subtilis, P. aeruginosa, A. flavus and C. albicans. The Mn3O4 NPs inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 40 μg/ml and C. albicans with a MIC of 15 μg/ml. Furthermore, the Mn3O4 NPs anti-cancer activity was examined using MTT essay against A549 lung and MCF-7 breast cancer cell lines. The Mn3O4 NPs revealed significant activity against the examined cancer cell lines A549 and MCF-7. The IC50 values of Mn3O4 NPs with A549 cell line was found at concentration of 98 µg/mL and MCF-7 cell line was found at concentration of 25 µg/mL. 相似文献
9.
El Achi Hanadi Awwad Johnny Abou Daya Sarah Halabi Sahar Damianos Sandra Mahfouz Rami 《Molecular biology reports》2018,45(5):911-916
Molecular Biology Reports - Recurrent pregnancy loss (RPL) is a problem affecting up to 5% of women of childbearing age due to many factors. Studies have shown that RPL and cardiovascular disease... 相似文献
10.
Alghzzawy Zakaria Mohamed Elmaghraby Tarek Khaled El-Hamid Hagag Sanaa Abd Awwad Mohammed Hussein 《Molecular biology reports》2020,47(3):1883-1893
Molecular Biology Reports - Hepatocellular carcinoma (HCC) is a tough opponent. HCC contributes to 14.8% of all cancer mortality in Egypt. There are many choices for management of HCC; however... 相似文献