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Zilkah Shmuel Faingersh Evgenia Rotbaum Arie Dvorkin Rima Eilam Tamar Anikster Yehoshua 《Plant Cell, Tissue and Organ Culture》1999,59(3):209-215
Alternate host plants of cereal rust fungi are necessary for studying the rust sexual cycle and pathogenicity. These plants
are usually difficult to propagate through cloning, while seed-propagated plants may have variable responses to the pathogen.
To overcome these obstacles, tissue culture, under controlled and aseptic conditions, was utilized for clonal propagation
and in vitro inoculation of the following species: Rhamnus palaestinus Boiss., the alternate host of oat (Avena spp.) crown rust (Puccinia coronata Corda); Thalictrum speciosissimum L., the alternate host of brown leaf rust of wheat (Puccinia recondita f. sp. tritici Eriks. & Henn.); and Lycopsis arvensis L., the alternate host of rye (Secala spp.) leaf rust (Puccinia recondita f. sp. recondita Rob. & Desm.). Shoot culture procedures for initial establishment and proliferation were developed for all three alternate
host species. Shoot cultures were multiplied at rates ranging from 0.3 to 1.7 shoots/week. Successful infection following
inoculation with teliospores of the corresponding rust fungi was obtained for R. palaestinus and T. speciosissimum but not for L. arvensis. The hardening and acclimatization efficiency of rooted T. speciosissimum and L. arvensis was of 80–90%. The propagation efficiency for R. palaestinus was not successful because of the low rate and poor quality of its rooting. It is concluded that the in vitro system might be used as an alternative method for inoculation and multiplication of alternate hosts of cereal rusts, although
more experimentation is needed to define accurately the appropriate conditions for the proper infection response.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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