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Medvedeva NV Mukhtarova LS Mukhitova FK Balandina AA Latypov SK Grechkin AN 《Chemistry and physics of lipids》2007,148(2):91-96
Allene oxide, (9Z,11E)-12,13-epoxy-9,11-octadecadienoic acid (12,13-EOD), was prepared by incubation of linoleic acid (13S)-hydroperoxide with flaxseed allene oxide synthase (AOS) and purified (as methyl ester) by low temperature HPLC. Identification of pure 12,13-EOD was substantiated by its UV and (1)H NMR spectra and by GC-MS data for its methanol trapping product. The methyl ester of 12,13-EOD (but not the free carboxylic acid) is slowly cyclized in hexane solution, affording a novel cyclopentenone cis-12-oxo-10-phytoenoic acid. Free carboxylic form of 12,13-EOD does not cyclize due to the exceeding formation of macrolactone (9Z)-12-oxo-9-octadecen-11-olide. The spontaneous cyclization of pure natural allene oxide (12,13-EOD) into cis-cyclopentenone have been observed first time. 相似文献
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Although bacteria from the genus Collimonas have demonstrated in vitro antifungal activity against many different fungi, they appeared inactive against the plant-pathogenic fungus Fusarium oxysporum f.sp. radicis-lycopersici (Forl), the causal agent of tomato foot and root rot (TFRR). Visualization studies using fluorescently labelled organisms showed that bacterial cells attached extensively to the fungal hyphae under nutrient-poor conditions but not in glucose-rich Armstrong medium. Collimonas fungivorans was shown to be as efficient in colonizing tomato root tips as the excellent colonizer Pseudomonas fluorescens strain WCS365. Furthermore, it appeared to colonize the same sites on the root as did the phytopathogenic fungus. Under greenhouse conditions in potting soil, C. fungivorans performed as well in biocontrol of TFRR as the well-established biocontrol strains P. fluorescens WCS365 and Pseudomonas chlororaphis PCL1391. Moreover, under biocontrol conditions, C. fungivorans did not attach to Forl hyphae colonizing plant roots. Based on these observations, we hypothesize that C. fungivorans mainly controls TFRR through a mechanism of competition for nutrients and niches rather than through its reported mycophagous properties, for which attachment of the bacteria to the fungal hyphae is assumed to be important. 相似文献
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Vikhanskaya F Toh WH Dulloo I Wu Q Boominathan L Ng HH Vousden KH Sabapathy K 《Nature cell biology》2007,9(6):698-705
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Artem S Novozhilov Faina S Berezovskaya Eugene V Koonin Georgy P Karev 《Biology direct》2006,1(1):6-18
Background
Oncolytic viruses that specifically target tumor cells are promising anti-cancer therapeutic agents. The interaction between an oncolytic virus and tumor cells is amenable to mathematical modeling using adaptations of techniques employed previously for modeling other types of virus-cell interaction. 相似文献8.
Georgy P Karev Yuri I Wolf Andrey Y Rzhetsky Faina S Berezovskaya Eugene V Koonin 《BMC evolutionary biology》2002,2(1):18-26
Background
Power distributions appear in numerous biological, physical and other contexts, which appear to be fundamentally different. In biology, power laws have been claimed to describe the distributions of the connections of enzymes and metabolites in metabolic networks, the number of interactions partners of a given protein, the number of members in paralogous families, and other quantities. In network analysis, power laws imply evolution of the network with preferential attachment, i.e. a greater likelihood of nodes being added to pre-existing hubs. Exploration of different types of evolutionary models in an attempt to determine which of them lead to power law distributions has the potential of revealing non-trivial aspects of genome evolution. 相似文献9.
A double-species ecologo-genetical model, including Drosophila and yeast, has been used as a new methodological instrument for investigation of the physiological mechanism of recombination. Incubation of Drosophila females in the medium containing yeast of the strain mutant for ergosterol synthesis leads to suppression of temperature-induced crossing over. The mass-spectrum analysis of steroid fraction from Drosophila females has shown that incubation of the yeast medium without ergosterol results in arrest of ecdysterone synthesis. These data are explained by the absence of ecdysterone synthesis precursor in the fly organism. The endocrinal control of crossing over is discussed in the light of hormonal regulation of meiosis. 相似文献
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M. Faina V. Billat R. Squadrone M. De Angelis J. P. Koralsztein A. Dal Monte 《European journal of applied physiology and occupational physiology》1997,76(1):13-20
Using 23 elite male athletes (8 cyclists, 7 kayakists, and 8 swimmers), the contribution of the anaerobic energy system to
the time to exhaustion (t
lim) at the minimal exercise intensity (speed or power) at which maximal oxygen uptake (V˙O2
max) occurs (I
V˙O2
max) was assessed by analysing the relationship between the t
lim and the accumulated oxygen deficit (AOD). After 10-min warming up at 60% of V˙O2
max, the exercise intensity was increased so that each subject reached his I
V˙O2max
in 30 s and then continued at that level until he was exhausted. Pre-tests included a continuous incremental test with 2 min
steps for determining the I
V˙O2max
and a series of 5-min submaximal intensities to collect the data that would allow the estimation of the energy expenditure
at I
V˙O2max
. The AOD for the t
lim exercise was calculated as the difference between the above estimation and the accumulated oxygen uptake. The mean percentage
value of energy expenditure covered by anaerobic metabolism was 15.2 [(SD 6)%, range 8.9–24.1] with significant differences
between swimmers and kayakists (16.8% vs 11.5%, P≤0.05) and cyclists and kayakists (16.4% vs 11.5%, P≤0.05). Absolute AOD values ranged from 26.4 ml · kg−1 to 83.6 ml · kg−1 with a mean value of 45.9 (SD 18) ml · kg−1. Considering all the subjects, the t
lim was found to have a positive and significant correlation with AOD (r = 0.62, P≤0.05), and a negative and significant correlation with V˙O2
max (r = −0.46, P≤0.05). The data would suggest that the contribution of anaerobic processes during exercise performed at I
V˙O2max
should not be ignored when t
lim is used as a supplementary parameter to evaluate specific adaptation of athletes.
Accepted: 17 December 1996 相似文献