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1.
The two-faced nature of protozoa allowed us to study the relationship between cell cycle and behaviour in Euplotes crassus; the former represents one of the most typical cellular traits, the latter is one of the most significant characteristics of an organism. Dividing cells of E. crassus were isolated on a slide and recorded for 11 h: the classic ethographic parameters were calculated every 60 min. The percentage of mobile cells went from 0-100% in the first 2.5 h. This value was maintained for 6.5 h, but from 9 h the value began to drop, reaching 0% at 11 h. The relative frequency of leftward arcs was very high in the first hour, the radius and length of the arcs increased from 1-7 h; velocity showed a similar trend, peaking at 7 h. All our results showed that the behaviour of this ciliate is heavily affected by its cell-division cycle. 相似文献
2.
Petraccone L Erra E Nasti L Galeone A Randazzo A Mayol L Barone G Giancola C 《International journal of biological macromolecules》2003,31(4-5):131-137
Telomeric guanine-rich sequence can adopt quadruplex structures that are important for their biological role in chromosomal stabilisation. G quartets are characterised by the cyclic hydrogen bonding of four guanine bases in a coplanar arrangement and their stability is ion-dependent. In this work we compare the stability of [d(TGGGT)]4 and [d(T*GGGT)]4 quadruplexes. The last one contains a modified thymine, where the hydroxyl group substitutes one hydrogen atom of the methyl group of the thymine in the [d(TGGGT)]4 sequence. We used a combination of spectroscopic, calorimetric and computational techniques to characterise the G-quadruplex formation. NMR and CD spectra of [d(T*GGGT)]4 were characteristic of parallel-stranded, tetramolecular quadruplex. CD and DSC melting experiments reveal that [d(T*GGGT)]4 is less stable that unmodified quadruplex. Molecular models suggest possible explanation for the observed behaviour. 相似文献
3.
D'Onofrio J Petraccone L Erra E Martino L Fabio GD Napoli LD Giancola C Montesarchio D 《Bioconjugate chemistry》2007,18(4):1194-1204
Oligodeoxyribonucleotides of sequence d(5'TGGGAG3') carrying bulky aromatic groups at the 5' end were found to exhibit potent anti-HIV activity [Hotoda, H., et al. (1998) J. Med. Chem. 41, 3655-3663 and references therein]. Structure-activity relationship investigations indicated that G-quadruplex formation, as well as the presence of large aromatic substituents at the 5'-end, were both essential for their antiviral activity. In this work, we synthesized some representative examples of the anti-HIV active Hotoda's 6-mers and analyzed the resulting G-quadruplexes by CD, DSC, and molecular modeling studies, in comparison with the unmodified oligonucleotide. In the case of the sequence carrying the 3,4-dibenzyloxybenzyl (DBB) group, identified as the best candidate for further drug optimization, we developed an alternative protocol to synthesize the 5'-DBB-thymidine phosphoramidite building block in higher yields. The thermodynamic and kinetic parameters for the association/dissociation processes of the 5'-conjugated quadruplexes, determined with respect to the unmodified one, were discussed in light of the molecular modeling studies. The aromatic groups at the 5' position of d(5'TGGGAG3') dramatically enhance both the equilibrium and the rate of formation of the quadruplex complexes. The overall stability of the investigated quadruplexes was found to correlate with the reported IC50 values, thus furnishing quantitative evidence for the hypothesis that the G-quadruplex structures are the ultimate active species, effectively responsible for the biological activity. 相似文献
4.
Albani D Giorgetti L Pitto L Luo M Cantoni RM Erra Pujada M Rotino GL Cella R 《European journal of histochemistry : EJH》2005,49(2):107-115
The pattern of expression of a carrot dhfr-ts gene was evaluated in different plant organs, in somatic embryos, and in hypocotyl explants induced to dedifferentiate in vitro by the addition of the synthetic auxin 2,4 dichorophenoxyacetic acid. The promoter of this gene was also placed upstream of a uidA (GUS) reporter gene and, using biolistic and protoplasts transient expression assays, was shown to drive a particularly high level of expression in actively growing suspension cells. The results from these expression analyses combined with the presence of putative cell cycle-related cis-acting elements in the dhfr-ts promoter, strongly point to a cell division-dependent expression of this gene. 相似文献
5.
Esposito V Randazzo A Petraccone L Giancola C Erra E Virgilio A Mayol L 《Nucleosides, nucleotides & nucleic acids》2005,24(5-7):789-794
Truncated sequences of human telomeric DNA can readily assemble to form parallel stranded quadruplexes containing A- and G-tetrads. The formation of an A-tetrad is highly context-dependent and the relationship between the formation of an A-tetrad and the glycosidic torsion angle of the adenosine residues implicated has not been completely clarified so far. In order to give a further insight in this issue we synthesized the modified oligomers d(ABrGGGT) and d(TABrGGGT), two different truncations of the human telomeric sequence containing a 8-bromoadenosine residue, named ABr. NMR data show that both the modified oligomers are able to perfectly fold into highly symmetric quadruplexes with all strands parallel to each other. Molecular modeling studies were performed on both [d(ABrGGGT)]4 and [d(TABrGGGT)]4, indicating that a bulky substituent, such as a bromine atom at the C8 position of adenines, can force the glycosidic bond to adopt a syn conformation, stabilizing the resulting quadruplexes. 相似文献
6.
Petraccone L Erra E Messere A Montesarchio D Piccialli G De Napoli L Barone G Giancola C 《Biopolymers》2004,73(4):434-442
Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex. 相似文献
7.
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
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Soledad L. Saavedra María C. Martínez Ceron Silvana L. Giudicessi Guillermina Forno María Belén Bosco Mariela M. Marani Rosa Erra‐Balsells Fernando Albericio Osvaldo Cascone Silvia A. Camperi 《Biotechnology progress》2018,34(4):999-1005
Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:999–1005, 2018 相似文献
8.
G. Bonardel C. Barrau M. Soussan E. D’Estanque B. Erra C. Etard N. Fayard M.O. Habert S. Hapdey E. Hindie F. Hyafil C. Labriolle Vaylet O. Lairez P.Y. Leroux P. Olivier M. Queneau E. Rust R. Seban M. Wartski 《Médecine Nucléaire》2018,42(5):295-297
Nuclear medicine hybrid imaging is a technological evolution of gamma camera scintigraphy or positron emission tomography imaging methods that are now often coupled with an anatomical imaging device, essentially a CT scanner. Following a large demand from the nuclear physicians themselves, but also from the French Nuclear Safety Authority, this guide is intended for the entire nuclear medicine community to integrate both the aspects of radiation protection related to coupled CT and those related to the quality of the CT images according to the clinical context. 相似文献
9.
G. Bonardel C. Barrau M. Soussan E. D’Estanque B. Erra C. Etard N. Fayard M.O. Habert S. Hapdey E. Hindie F. Hyafil C. Labriolle Vaylet O. Lairez P.Y. Leroux P. Olivier M. Queneau E. Rust R. Seban M. Wartski 《Médecine Nucléaire》2018,42(5):298-313
Many parameters can affect the patient's dose and the CT image quality. It is therefore essential, in order to optimize the patient's dosimetry, to know the influence of these parameters as well as their link with the dose modulation tools. 相似文献
10.
G. Bonardel C. Barrau M. Soussan E. D’Estanque B. Erra C. Etard N. Fayard M.O. Habert S. Hapdey E. Hindie F. Hyafil C. Labriolle Vaylet O. Lairez P.Y. Leroux P. Olivier M. Queneau E. Rust R. Seban M. Wartski 《Médecine Nucléaire》2018,42(5):342-353
The examples and clinical cases presented in this section are not intended to be considered as absolute models in terms of image quality or device parameter settings. They must initiate an individual analysis according to CT parameters and image quality. Nevertheless, they present practically different CT levels, which can be used according to the clinical context and the type of device. 相似文献