全文获取类型
收费全文 | 3726篇 |
免费 | 302篇 |
出版年
2023年 | 19篇 |
2022年 | 56篇 |
2021年 | 103篇 |
2020年 | 48篇 |
2019年 | 63篇 |
2018年 | 96篇 |
2017年 | 75篇 |
2016年 | 124篇 |
2015年 | 210篇 |
2014年 | 222篇 |
2013年 | 278篇 |
2012年 | 323篇 |
2011年 | 311篇 |
2010年 | 200篇 |
2009年 | 168篇 |
2008年 | 239篇 |
2007年 | 231篇 |
2006年 | 194篇 |
2005年 | 179篇 |
2004年 | 163篇 |
2003年 | 171篇 |
2002年 | 145篇 |
2001年 | 28篇 |
2000年 | 33篇 |
1999年 | 30篇 |
1998年 | 40篇 |
1997年 | 24篇 |
1996年 | 21篇 |
1995年 | 19篇 |
1994年 | 19篇 |
1993年 | 17篇 |
1992年 | 20篇 |
1991年 | 19篇 |
1990年 | 18篇 |
1989年 | 16篇 |
1988年 | 12篇 |
1987年 | 18篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1978年 | 3篇 |
1973年 | 3篇 |
1972年 | 4篇 |
1969年 | 3篇 |
1968年 | 3篇 |
1966年 | 2篇 |
排序方式: 共有4028条查询结果,搜索用时 31 毫秒
1.
2.
Francesca Passaretta Domenico Bosco Lucia Centurione Maria Antonietta Centurione Fabio Marongiu Roberta Di Pietro 《Journal of cellular and molecular medicine》2020,24(7):4350-4355
Human Amniotic Epithelial Cells (hAEC) isolated from term placenta are a promising source for regenerative medicine. However, it has long been debated whether the hAEC population consists of heterogeneous or homogeneous cells. In a previous study, we investigated the characteristics of hAEC isolated from four different regions of the amniotic membrane finding significant heterogeneity. The aim of this study was to evaluate the hepatic differentiation capability of hAEC isolated from these four regions. Human term placentae were collected after caesarean section and hAEC were isolated from four regions of the amniotic membrane (R1-R4, according to their relative distance from the umbilical cord) and treated in hepatic differentiation conditions for 14 days. hAEC-derived hepatocyte-like cells showed marked differences in the expression of hepatic markers: R4 showed higher levels of Albumin and Hepatocyte Nuclear Factor (HNF) 4α whereas R1 expressed higher Cytochrome P450 enzymes, both at the gene and protein level. These preliminary results suggest that hAEC isolated from R1 and R4 of the amniotic membrane are more prone to hepatic differentiation. Therefore, the use of hAEC from a specific region of the amniotic membrane should be taken into consideration as it could have an impact on the outcome of therapeutic applications. 相似文献
3.
4.
Common signal transduction system shared by STE2 and STE3 in haploid cells of Saccharomyces cerevisiae: autocrine cell-cycle arrest results from forced expression of STE2 总被引:20,自引:3,他引:17
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Induction of STE2 expression using the GAL1 promoter both in a wild-type MATalpha strain and in a MATalpha ste3 strain caused transient cell-cycle arrest and changes in morphology ('shmoo'-like phenotype) in a manner similar to alpha cells responding to alpha-factor. In addition, STE2 expressed in a MATalp[ha ste3 mutant allowed the cell to conjugate with alpha cells but at an efficiency lower than that of wil-type alpha cells. This result indicates that signal(s) generated by alpha-factor in alpha cells can be substituted by signal(s) generated by the interaction of alpha-factor with the expressed STE2 product. When STE2 or STE3 was expressed in a matalpha1 strain (insensitive to both alpha- and a-factors), the cell became sensitive to alpha- or a-factor, respectively, and resulted in morphological changes. These results suggest that STE2 and STE3 are the sole determinants for alpha-factor and a-factor sensitivity, respectively, in this strain. On the other hand, expression of STE2 in an a/alpha diploid cell did not affect the alpha-factor insensitive phenotype. Haploid-specific components may be necessary to transduce the alpha-factor signal. These results are consistent with the idea that STE2 encodes an alpha-factor receptor and STE3 encodes an a-factor receptor, and suggest that both alpha- and a-factors may generate an exchangeable signal(s) within haploid cells. 相似文献
5.
B cell growth-promoting activity of recombinant human interleukin 4 总被引:23,自引:0,他引:23
T Defrance B Vanbervliet J P Aubry Y Takebe N Arai A Miyajima T Yokota F Lee K Arai J E de Vries 《Journal of immunology (Baltimore, Md. : 1950)》1987,139(4):1135-1141
Human interleukin 4 (IL-4), also known as B cell stimulatory factor 1, is a T cell-derived glycoprotein consisting of 129 amino acids for which a cDNA has been recently isolated. IL-4 displays little or no B cell growth factor (BCGF) activity in the standard anti-IgM costimulatory assay using suboptimal concentrations of soluble anti-IgM antibody whereas the low m.w. BCGF is very active. When insolubilized anti-IgM was used as the costimulating agent, both IL-4 and the low m.w. BCGF were found to promote B cell proliferation. Human IL-4 is able to induce the proliferation of B lymphocytes preactivated for either 1 day with insolubilized anti-IgM antibody or for 3 days with Staphylococcus aureus strain Cowan I. However, IL-4 is poorly mitogenic for B cells preactivated for 1 day with the Staphylococcus strain whereas the low m.w. BCGF strongly enhances the proliferation of these B cells. These two findings demonstrate that the preactivation signal necessary to induce human B cells to proliferate in response to IL-4 is critical. The increased tritiated thymidine ([3H]dThd) uptake in preactivated B cell cultures with IL-4 reflects cel proliferation because cell cycle analysis demonstrates that IL-4 induces activated B cells to enter the S and G2/M phases of the cell cycle and the addition of IL-4 to preactivated B cell cultures permits the recovery of three- to fourfold more B cells after 4 days of culture. IL-4 and the low m.w. BCGF act in concert to induce the proliferation of anti-IgM-preactivated B cells as demonstrated by [3H]dThd uptake and cell cycle analysis. In striking contrast to the demonstrated antagonistic effect of interferon-gamma on the IL-4-induced expression of the low affinity receptor for IgE (Fc epsilon RL/CD23), on B cells, it was found that interferon-gamma enhanced the IL-4-induced proliferation of anti-IgM-preactivated B cells. Finally, it was found that IL-4 had to be present continuously during the culture period to exert an optimal growth-promoting effect on B cell blasts. As a conclusion, IL-4 is able to induce the proliferation of an appropriately activated subpopulation of human B cells. 相似文献
6.
7.
8.
9.
T Oda H Miyajima Y Suzuki T Ito S Yokota M Hoshino A Ichiyama 《Journal of biochemistry》1989,106(3):460-467
In the previous study (Oda, T., et al. (1985) Eur. J. Biochem. 150, 415-421), we isolated a cDNA clone which expressed in Escherichia coli a specific size of product having the activity of rat liver serine:pyruvate aminotransferase (SPTm). This specific product (SPT10) was purified to homogeneity through three different column chromatographies. The amino acid composition and N-terminal amino acid sequence of the purified enzyme agreed with those predicted from the nucleotide sequence of cDNA and showed that SPT10 consists of the whole amino acid sequence of mature SPTm and several extra amino acid residues at the N-terminus. The catalytic and physical properties of SPT10, such as substrate specificity, Km for alpha-keto acids, electric charge, and quaternary structure, were all very similar to those of SPTm. Using several cDNA clones which lack a 5'-terminal sequence corresponding to a portion of the N-terminal amino acid sequence of SPTm, we examined the expression profile of the specific product in bacteria transformed with each cDNA clone. The products encoded by these cDNAs were segregated into inclusion bodies and were neither catalytically active nor easily solubilized by sonication. In contrast, the inclusion bodies were not formed in the bacteria transformed with the cDNA clone for SPT10. 相似文献
10.
Nicholas C. Nicolaides Fabio Palombo Kenneth W. Kinzler Bert Vogelstein Josef Jiricny 《Genomics》1996,31(3):395
Defects in mismatch repair genes cause the genetic instability characteristic of hereditary nonpolyposis colorectal cancer and a subset of sporadic colon tumors. The newest member of the mismatch repair gene family,GTBP, has recently been identified as a partial cDNA. Here, we describe the isolation of its 5′ terminus, allowing definition of the entire coding region. Several polymorphisms within the 5′ end were identified and are presented. 相似文献