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SYNOPSIS. The sustainable use of wild species by local peopleis emerging as an important conservation strategy. The premiseis the economic value of species will justify their own preservationas well as the habitats they occupy. However, the lack of naturalhistory and demographic information for the majority of speciesbeing exploited or with potential uses presents challengingproblems for implementing sustainable use programs. Each yearin Argentina and Paraguay, an average of 1.9 million tegu lizardsof the genus Tupinambis are exploited for their skins. In spiteof the importance of tegus as a resource, their biology is poorlyknown and their populations have never been managed. The lifehistory of Tupinambis, like that of other exploited lizards,is characterized by a relatively long life span, a large clutchsize, several years of growth before reproduction, and highmortality of hatchlings. Importantly, the mortality of young-of-the-yearand the proportion of females reproducing each year are bothprobably strongly influenced by interannual environmental variation.Whenthese parameters were randomized in life table projections tosimulate the effects of environmental stochasticity, the populationgrowth rate was highly sensitive to environmental fluctuations.Monte Carlo simulations of different harvest strategies showedthat estimates of population growth rates were overwhelminglyinfluenced by environmental variation and the number of yearsincluded in the growth rate estimate, even in the face of seeminglylarge changes in adult mortality that would result from populationmanagement. These results are both encouraging and cautionaryfor Tupinambis conservation. On the one hand these patternscan help explain how Tupinambis populations may have persistedin spite of high and variable harvest levels during many years,but conversely, stochastic effects make it difficult to evaluatethe effects of conservation measures. Size and sex can be determinedfrom harvested skins, and pilot studies suggest that analysesof the annual harvest can provide valuable information for evaluatinglong-term population trends. 相似文献
4.
Variation in heat shock proteins within tropical and desert species of poeciliid fishes 总被引:8,自引:0,他引:8
Norris CE; diIorio PJ; Schultz RJ; Hightower LE 《Molecular biology and evolution》1995,12(6):1048-1062
The 70-kilodalton heat shock protein (hsp70) family of molecular
chaperones, which contains both stress-inducible and normally abundant
constitutive members, is highly conserved across distantly related taxa.
Analysis of this protein family in individuals from an outbred population
of tropical topminnows, Poeciliopsis gracilis, showed that while
constitutive hsp70 family members showed no variation in protein isoforms,
inducibly synthesized hsp70 was polymorphic. Several species of
Poeciliopsis adapted to desert environments exhibited lower levels of
inducible hsp70 polymorphism than the tropical species, but constitutive
forms were identical to those in P. gracilis, as they were in the
confamilial species Gambusia affinis. These differences suggest that
inducible and constitutive members of this family are under different
evolutionary constraints and may indicate differences in their function
within the cell. Also, northern desert species of Poeciliopsis synthesize a
subset of the inducible hsp70 isoforms seen in tropical species. This
distribution supports the theory that ancestral tropical fish migrated
northward and colonized desert streams; the subsequent decrease in
variation of inducible hsp70 may have been due to genetic drift or a
consequence of adaptation to the desert environment. Higher levels of
variability were found when the 30- kilodalton heat shock protein (hsp30)
family was analyzed within different strains of two desert species of
Poeciliopsis and also in wild-caught individuals of Gambusia affinis. In
both cases the distribution of hsp30 isoform diversity was similar to that
seen previously with allozyme polymorphisms.
相似文献
5.
Control of cell volume in the J774 macrophage by microtubule disassembly and cyclic AMP 总被引:8,自引:5,他引:3 下载免费PDF全文
We have explored the possibilities that cell volume is regulated by the status of microtubule assembly and cyclic AMP metabolism and may be coordinated with shape change. Treatment of J774.2 mouse macrophages with colchicine caused rapid microtubule disassembly and was associated with a striking increase (from 15-20 to more than 90 percent) in the proportion of cells with a large protuberance at one pole. This provided a simple experimental system in which shape changes occurred in virtually an entire cell population in suspension. Parallel changes in cell volume could then be quantified by isotope dilution techniques. We found that the shape change caused by colchicine was accompanied by a decrease in cell volume of approximately 20 percent. Nocodozole, but not lumicolchicine, caused identical changes in both cell shape and cell volume. The volume loss was not due to cell lysis nor to inhibition of pinocytosis. The mechanism of volume loss was also examined. Colchicine induced a small but reproducible increase in activity of the ouabain-sensitive Na(+), K(+)-dependent ATPase. However, inhibition of this enzyme/transport system by ouabain did not change cell volume nor did it block the colchicines-induced decrease in volume. One the other hand, SITS (4’acetamido, 4-isothiocyano 2,2’ disulfonic acid stilbene), an inhibitor of anion transport, inhibited the effects of colchicines, thus suggesting a role for an anion transport system in cell volume regulation. Because colchicine is known to activate adenylate cyclase in several systems and because cell shape changes are often induced by hormones that elevate cyclic AMP, we also examined the effects of cyclic AMP on cell volume. Agents that act to increase syclic AMP (cholera toxin, which activates adenylate cyclase; IBMX, and inhibitor of phosphodiesterase; and dibutyryl cyclic AMP) all caused a volume decrease comparable to that of colchicine. To define the effective metabolic pathway, we studied two mutants of J774.2, one deficient in adenylate cyclase and the other exhibiting markedly reduced activity of cyclic AMP-dependent protein kinase. Cholera toxin did not produce a volume change in either mutant. Cyclic AMP produced a decrease in the cyclase-deficient line comparable to that in wild type, but did not cause a volume change in the kinase- deficient line. This analysis established separate roles for cyclic AMP and colchicine. The volume decrease induced by cyclic AMP requires the action of a cyclic AMP-dependent protein kinase. Colchicine, on the other hand, induced a comparable volume change in both mutants and wild type, and thus does not require the kinase. 相似文献
6.
PAUL R. FITZGERALD 《The Journal of eukaryotic microbiology》1965,12(2):215-221
SYNOPSIS. Five experiments using newborn Holstein-Friesian and weaner Hereford calves were conducted to observe the effects caused by parenteral injections of oocysts of Eimeria bovis . Sporulated oocysts were given intraperitoneally (IP), subcutaneously (SC), intramuscularly (IM) and intravenously (IV). Unsporulated oocysts or merozoites were given IP or IM.
Coccidiosis developed in calves in three experiments after they were inoculated IP with sporulated oocysts. Immunity to reinfection resulted from these infections. No infections occurred at any time after SC, IM or IV inoculation with sporulated oocysts or after IP or IM inoculation with unsporulated oocysts or merozoites.
Coccidiosis failed to occur in two experiments when special precautions were used to prevent puncture of the intestines during IP inoculations. There was no detectable immunological response to any of the inoculations unless intestinal infections occurred.
In one experiment sporulated oocysts were exposed to 60,000 r irradiation by x-ray in an attempt to attenuate the oocysts. Calves became infected when given orally administered oocysts irradiated at this level. 相似文献
Coccidiosis developed in calves in three experiments after they were inoculated IP with sporulated oocysts. Immunity to reinfection resulted from these infections. No infections occurred at any time after SC, IM or IV inoculation with sporulated oocysts or after IP or IM inoculation with unsporulated oocysts or merozoites.
Coccidiosis failed to occur in two experiments when special precautions were used to prevent puncture of the intestines during IP inoculations. There was no detectable immunological response to any of the inoculations unless intestinal infections occurred.
In one experiment sporulated oocysts were exposed to 60,000 r irradiation by x-ray in an attempt to attenuate the oocysts. Calves became infected when given orally administered oocysts irradiated at this level. 相似文献
7.
Mesenchymal stromal cell (MSC) therapies have been pursued for a broad spectrum of indications but mixed reports on clinical efficacy have given rise to some degree of skepticism regarding the effectiveness of this approach. However, recent reports of successful clinical outcomes and regulatory approvals for graft-versus-host disease, Crohn's disease and critical limb ischemia have prompted a shift in this perspective. With hundreds of clinical trials involving MSCs currently underway and an increasing demand for large-scale manufacturing protocols, there is a critical need to develop standards that can be applied to processing methods and to establish consensus assays for both MSC processing control and MSC product release. Reference materials and validated, uniformly applied tests for quality control of MSC products are needed. Here, we review recent developments in MSC manufacturing technologies, release testing and potency assays. We conclude that, although MSCs hold considerable promise clinically, economies of scale have yet to be achieved although numerous bioreactor technologies for scalable production of MSCs exist. Additionally, rigorous disease-specific product testing and comprehensive understanding of mechanisms of action, which are linked to relevant process and product release potency assays, will be required to ensure that these therapies continue to be successful. 相似文献
8.
A second gene at the tomato Cf-4 locus confers resistance to cladosporium fulvum through recognition of a novel avirulence determinant 总被引:3,自引:0,他引:3
Takken FL Thomas CM Joosten MH Golstein C Westerink N Hille J Nijkamp HJ De Wit PJ Jones JD 《The Plant journal : for cell and molecular biology》1999,20(3):279-288
The tomato Cf-4 and Cf-9 genes confer resistance to the leaf mould pathogen Cladosporium fulvum and map at a complex locus on the short arm of chromosome 1. It was previously shown that the gene encoding Cf-4, which recognizes the Avr4 avirulence determinant, is one of five tandemly duplicated homologous genes (Hcr9-4s) at this locus. Cf-4 was identified by molecular analysis of rare Cf-4/Cf-9 disease-sensitive recombinants and by complementation analysis. The analysis did not exclude the possibility that an additional gene(s) located distal to Cf-4 may also confer resistance to C. fulvum. We demonstrate that a number of Dissociation-tagged Cf-4 mutants, identified on the basis of their insensitivity to Avr4, are still resistant to infection by C. fulvum race 5. Molecular analysis of 16 Cf-4 mutants, most of which have small chromosomal deletions in this region, suggested the additional resistance specificity is encoded by Hcr9-4E. Hcr9-4E recognizes a novel C. fulvum avirulence determinant that we have designated Avr4E. 相似文献
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10.
PJ?MumbyEmail author JD?Hedley JRM?Chisholm CD?Clark H?Ripley J?Jaubert 《Coral reefs (Online)》2004,23(2):171-183
Trends in coral cover are widely used to indicate the health of coral reefs but are costly to obtain from field survey over large areas. In situ studies of reflected spectra at the coral surface show that living and recently dead colonies can be distinguished. Here, we investigate whether such spectral differences can be detected using an airborne remote sensing instrument. The Compact Airborne Spectrographic Imager (Itres Research Ltd, Canada) was flown in two configurations: 10 spectral bands with 1-m2 pixels and 6 spectral bands with 0.25-m2 pixels. First, we show that an instrument with 10 spectral bands possesses adequate spectral resolution to distinguish living Porites, living Pocillopora spp., partially dead Porites, recently dead
Porites (total colony mortality within 6 months), old dead (>6 months) Porites,
Halimeda spp., and coralline red algae when there is no water column to confuse spectra. All substrata were distinguished using fourth-order spectral derivatives around 538 nm and 562 nm. Then, at a shallow site (Tivaru) at Rangiroa Atoll, Tuamotu Archipelago (French Polynesia), we show that live and dead coral can be distinguished from the air to a depth of at least 4 m using first- and fourth-order spectral derivatives between 562–580 nm. However, partially dead and recently dead Porites
colonies could not be distinguished from an airborne platform. Spectral differences among substrata are then exploited to predict the cover of reef substrata in ten 25-m2 plots at nearby Motu Nuhi (max depth 8 m). The actual cover in these plots was determined in situ using quadrats with a 0.01-m2 grid. Considerable disparity occurred between field and image-based measures of substrate cover within individual 25-m2 quadrats. At this small scale, disparity, measured as the absolute difference in cover between field and remote-sensing methods, reached 25% in some substrata but was always less than 10% for living coral (99% of which consisted of
Porites spp.). At the scale of the reef (all ten 25-m2 quadrats), however, disparities in percent cover between imagery and field data were less than 10% for all substrata and extremely low for some classes (e.g. <3% for living
Porites, recently dead Porites
and Halimeda). The least accurately estimated substrata were sand and coralline red algae, which were overestimated by absolute values 7.9% and 6.6%, respectively. The precision of sampling was similar for field and remote-sensing methods: field methods required 19 plots to detect a 10% difference in coral cover among three reefs with a statistical power of 95%. Remote-sensing methods required 21 plots. However, it took 1 h to acquire imagery over 92,500 m2 of reef, which represents 3,700 plots of 25 m2 each, compared with 3 days to survey 10 such plots underwater. There were no significant differences in accuracy between 1-m2 and 0.25-m2 image resolutions, suggesting that the advantage of using smaller pixels is offset by reduced spectral information and an increase in noise (noise was observed to be 1.6–1.8 times greater in 0.25-m2 pixels). We show that airborne remote sensing can be used to monitor coral and algal cover over large areas, providing that water is shallow and clear, and that brown fleshy macroalgae are scarce, that depth is known independently (e.g. from sonar survey). 相似文献