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PIETRO CENINI ANDREA BOLOGNESI FIORENZO STIRPE 《The Journal of eukaryotic microbiology》1988,35(3):384-387
Ribosomes from Trypanosoma brucei rhodesiense and from Leishmania infantum were isolated and optimal conditions for in vitro translation were established. The effect of ribosome-inactivating proteins extracted from several plants was then assessed in order to identify those suitable for the preparation of immunotoxins against these organisms. Ribosomes from both species were inactivated by some ribosome-inactivating proteins (dianthins, saporins, pokeweed antiviral proteins, and the ribosome-inactivating chain of abrin). The similarity of the effects on the ribosomes from the two species examined indicates that ribosome-inactivating proteins should also be effective in a similar way on ribosomes from other species of Trypanosoma and Leishmania. 相似文献
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BATTELLI MARIA GIULIA; LORENZONI ENZO; STIRPE FIORENZO; CELLA RINO; PARISI BRUNO 《Journal of experimental botany》1984,35(6):882-889
The effect of ribosome-inactivating proteins (RIPs), eithersingle-chain or toxins, was studied on plant ribosomes. RIPsdid not affect ribosomes from their own plants, while inhibitingto a variable extent protein synthesis by heterologous plantribosomes. Ricin stimulated and PAPS inhibited the growthof carrot cells in culture. Key words: Plant ribosomes, Ribosome-inactivating proteins, Protein synthesis, Ribosome specificity, Plant cell cultures 相似文献
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