全缘叶绿绒蒿挥发性组分定量结构-色谱保留关系研究

提出顶点及顶点相互作用矢量的概念,并将该矢量用于复杂样本的分子结构表征。采用逐步回归结合统计检测对变量进行筛选后,再用多元线性回归建立了定量结构-色谱保留(QSRR)关系的7变量模型,模型的建模计算值复相关系数(R)为0.990,标准偏差(SD)为1.325;留一法(LOO)交互检验复相关系数(RCV)为0.983,标准偏差(SDCV)为1.696。结果表明该矢量具有较强的分子结构表达能力,模型具有良好的估计能力与稳定性。     

A new synonym of Pedicularis cyathophylla (Orobanchaceae)

Pedicularis cyathophylla Franchet in Bull.Soc.Bot.France 47:25.1900.Type:China.Sichuan:Kangding,Zheduoshan Mountains (Ta-tsien-kou & Tche-to-chan),J.A.Soulié 218 (holotype,P00160827,P!).Pedicularis xiangchengensis H.P.Yang in Acta Phytotax.Sin.28:137.f.3:3.1990,syn.nov.Type:China.Sichuan:Xiangcheng,Rewu,on mountain slopes,alt.4100-4300 m,1981-08-06,Qing-Zang Exped.3829(holotype,00032964,PE!;isotypes,KUN!,CDBI!).     

九节龙皂苷诱导胶质瘤SHG-44细胞凋亡的实验观察

目的：探讨九节龙皂苷对胶质瘤SHG-44细胞潜在的治疗作用及其机制。方法：用四基偶唑蓝（MTT）法检测5、7．5、10、12．5、15、20、40、80mg／L九节龙皂苷作用6、12、24、72h对人胶质瘤SHG-44细胞活性的影响和细胞流式术检测SGH-44细胞调亡情况;Hoeehst33258荧光染色法观察细胞形态的变化;琼脂糖凝胶电泳检测SHG-44细胞DNA的完整性。结果：九节龙皂苷明显抑制SHG-44细胞生长活性呈浓度-时间依赖性,并诱导细胞发生明显的凋亡,细胞核发生浓聚边集,DNA呈凋亡特异性“梯状”分布。结论：九节龙皂苷明显抑制SHG-44细胞的生长活性,能引起胶质瘤细胞大量凋亡,具有显著的抗肿瘤作用。     

Adenovirus-mediated expression of pig α(1,3) galactosyltransferase reconstructs Gal α(1,3) Gal epitope on the surface of human tumor cells

INTRODUCTIONGal a(1, 3) Gal (gal epitope) is a carbohydrate epitope, which is produced in large amounton the cells of pigs, mice and New World monkey(monkey of South America) by the glycosylationenzyme G alal 1 ) 4G IcNAc3- a- D- galactosyltransferase[or(1, 3)GT; EC2.4.1.511111. This enzyme is active in the Golgi appaxatus of cells and transfers galactose from the sugandonor uridine diphoSphate galactose (UDP-galactose) to the acceptor Nacetyllactosamine residue (Galaal-4GlcNAc-R…     

中国东北地区人乳头瘤病毒16型全基因组克隆及HPV16.HaCaT重组细胞模型的构建及初步鉴定

为构建含东北地区人乳头瘤病毒16型(HPV16)全基因组的HPV16.HaCaT细胞模型,收集中国东北地区HPV16单一感染患者宫颈脱落细胞,提取DNA,将HPV16全基因组分成4个区段,通过4对特异性引物对HPV16全基因组进行分段扩增,测序后进行序列拼接及核酸序列分析,克隆HPV16全基因组序列;通过细胞转染,构建含HPV16全基因组的HPV16.HaCaT重组细胞模型;利用聚合酶链式反应(PCR)和细胞免疫荧光法检测重组细胞内HPV16早期基因的表达.成功克隆出中国东北地区HPV16全基因组序列(GenBank登录号:MW320358);构建了东北地区HPV16全基因组的重组质粒及HPV16.HaCaT重组细胞模型;证明了 HPV16早期基因E1-E4、E5、E6和E7在重组细胞模型内均有表达,从而获得中国东北地区HPV16全基因组序列及含有HPV16全基因组的HPV16.HaCaT重组细胞模型.     

Competition, resource partitioning and coexistence of an endoparasitoid Encarsia perniciosi and an ectoparasitoid Aphytis melinus of the California red scale

Abstract. 1. Laboratory experiments and field studies were conducted to explain the coexistence of an endoparasitoid, Encarsia perniciosi Tower, and an ectoparasitoid, Aphytis melinus DeBach, both of which were introduced into California to control the California red scale, Aonidiella aurantii (Mask.).
2. Encarsia parasitized all scale stages but it preferred first and second instar scales. This is in contrast to Aphytis melinus , in which previous studies have shown that it parasitizes second and third instar females and second instar males but prefers third instar female scales. Encarsia developed most rapidly when it parasitized an early second instar and slowest when it parasitized the mature female scale. However, on early second instar scales it was about 80% as fecund as a wasp that emerged from a mature female scale.
3. Second instar scales parasitized by Encarsia were accepted by Aphytis as readily as unparasitized scales.
4. Encarsia did not distinguish between unparasitized hosts and those previously parasitized by Aphytis.
5. Encarsia always outcompeted by Aphytis when both species parasitize the same host.
6. Encarsia prefers scale on stems whereas Aphytis prefers those on leaves and fruits. This, too, may be a result of interspecific competition with Aphytis.
7. The partitioning of the scale resource by the two species explains why they coexist in coastal southern California but it does not explain why Encarsia disappeared from citrus groves in the inland valleys coincident with the introduction of Aphytis melinus into southern California.     

具有保肝作用的天然药物开发进展

肝脏是机体代谢的最主要场所,也是机体最容易遭受到损伤的脏器之一,各种因素引起的肝损伤已成为威胁人类健康的重要疾病之一。肝损伤机制主要与线粒体损伤、自由基脂质过氧化、炎症细胞因子分泌和细胞膜损伤有关。目前已报道很多天然药物具有显著保肝作用,且具有疗效稳定、副作用低、多途径作用、作用温和持久等优势,已广泛用于肝脏疾病的防治。本文对肝损伤的生理机制以及具有保肝作用的天然药物开发进展进行了综述,提出了目前存在的一些问题并进行了展望。     

蛹虫草菌丝生长研究

通过对 4种不同来源的蛹虫草菌种 ,在 5种不同配方培养基上菌丝生长情况进行了比较研究。结果表明 ,不同培养基配方对不同来源的蛹虫草菌种培养菌丝的长速和长势存在较大的差异。     

三羟异黄酮对HER-2/neu高表达乳腺癌细胞血管生成相关因子表达的影响

三羟异黄酮(genistein)是大豆中的一种非营养成分,其结构与黄酮化合物类似,能竞争性地与雌激素受体结合,故称之为植物雌激素(phytoestrogen)。它具有广泛的生物学作用,如抗肿瘤、抗病毒、抗真菌、抗氧化、抗突变、抗高血压、抗增生等,其中genistein抑制肿瘤的血管生成是当前研究的热点之一。肿瘤的血管生成是肿瘤进一步生长转移的基础,该过程受肿瘤细胞和血管内皮细胞分泌的血管生成相关因