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AV Shevchenko IG Budzanivska TP Shevchenko VP Polischuk D Spaar 《Archives Of Phytopathology And Plant Protection》2013,46(2):139-146
The work was focused on the investigation of possible dependencies between the development of viral infection in plants and the presence of high heavy metal concentrations in soil. Field experiments have been conducted in order to study the development of systemic tobacco mosaic virus (TMV) infection in Lycopersicon esculentum L. cv. Miliana plants under effect of separate salts of heavy metals Cu, Zn and Pb deposited in soil. As it is shown, simultaneous effect of viral infection and heavy metals in tenfold maximum permissible concentration leads to decrease of total chlorophyll content in experiment plants mainly due to the degradation of chlorophyll a. The reduction of chlorophyll concentration under the combined influence of both stress factors was more serious comparing to the separate effect of every single factor. Plants' treatment with toxic concentrations of lead and zinc leaded to slight delay in the development of systemic TMV infection together with more than twofold increase of virus content in plants that may be an evidence of synergism between these heavy metal's and virus' effects. Contrary, copper although decreased total chlorophyll content but showed protective properties and significantly reduced amount of virus in plants. 相似文献
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Katalymov LL Evstigneev DA 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2004,90(2):181-186
When cathode subthreshold impulse was turned off, excitable membranes of isolated nerve fibres and nervous trunk show postelectrotonic depolarisation (PED), that is a slow recovery of membrane potential to the resting level. PED of the single nodes of Ranvier and nervous trunk is registered not only in normal conditions, but also after complete block of sodium channels. The size and duration of nervous trunk PED under subthreshold depolarising current increase along with duration of applied depolarisation: when cathode current 1 ms in duration was used, they were 0.093 +/- +/- 0.004 mV and 7.123 +/- 0.576 ms, respectively; when current was 5 ms in duration, they were 0.189 +/- 0.005 mV and 23.212 +/- 1.186 ms, whereas a 10-ms depolarisation yields values of 0.220 +/- 0.011 mV and 68.721 +/- 3.389 ms. Application of the train of catelectrotonic impulses leads to PED built-up. As PED is found not only in normal conditions but also after complete block of sodium channels, it is reasonable to suggest that the most probable reason for PED is an outward potassium current. 相似文献
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Generalization of current views on energy analysis of complex formation of biologically active compounds with nucleic acids is represented and the outlook for further developments is determined. 相似文献
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Fabrizio Grandi Marcia M Colodel Lidianne N Monteiro João Rafael VP Leão Noeme S Rocha 《BMC veterinary research》2010,6(1):45
Backgroud
Extramedullary hematopoiesis (EMH) is defined as the presence of hematopoietic stem cells such as erythroid and myeloid lineage plus megakaryocytes in extramedullary sites like liver, spleen and lymph nodes and is usually associated with either bone marrow or hematological disorders. Mammary EMH is a rare condition either in human and veterinary medicine and can be associated with benign mixed mammary tumors, similarly to that described in this case. 相似文献8.
The competitive binding of anthracycline antitumour drugs, [daunomycin (DAU), doxorubicin (DOX) or nogalamycin (NOG)], with
caffeine (CAF) to a model DNA oligomer has been investigated by 500 MHz 1H NMR spectroscopy under physiological solution conditions. The method depends on the stepwise analysis of one-component (self-association),
two-component (hetero-association and DNA complexation) and three-component interactions, in order to de-convolute the overall
binding of the anthracycline antibiotic and CAF to DNA into two competing processes, viz. hetero-association of the antibiotic-CAF
(‘interceptor’ action of CAF) and CAF–DNA complexation (‘protector’ action of CAF). It is found that the complexation of DAU
with DNA in the presence of CAF is mainly affected by the CAF–DNA complexation, whereas the binding of either DOX or NOG to
DNA is affected approximately equally by both the CAF–DNA complexation and CAF-antibiotic hetero-association. Quantitative
evaluation of the three-component mixture of drug–CAF–DNA has enabled the proportion of the antibiotic displaced from DNA
on addition of CAF to be calculated over a large range of CAF concentration, which may provide a quantitative basis for the
change in anthracycline-related toxicity on addition of CAF. 相似文献
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The analysis of the energy contributions of various physical factors to the complex formation between biologically active compounds and nucleic acids in aqueous solution was performed. A comparison of the energy parameters was made for ligand-ligand, intercalator-DNA, MGB-DNA and ligand-RNA groups. It was shown that the energetics of these reactions is of compensatory nature. Physical factors exerting the most pronounced influence on the energy parameters were identified. Correlation of the energy contributions to MGB-DNA complex formation and its biological effect was found. 相似文献