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Joni Esrom Lima Vagner Augusto Benedito Antonio Figueira Lázaro Eustáquio Pereira Peres 《Plant cell reports》2009,28(8):1169-1177
We analyzed the impact of ethylene and auxin disturbances on callus, shoots and Agrobacterium rhizogenes-induced hairy root formation in tomato (Solanum lycopersicum L.). The auxin low-sensitivity dgt mutation showed little hairy root initiation, whereas the ethylene low-sensitivity Nr mutation did not differ from the control Micro-Tom cultivar. Micro-Tom and dgt hairy roots containing auxin sensitivity/biosynthesis rol and aux genes formed prominent callus onto media supplemented with cytokinin. Under the same conditions, Nr hairy roots did not form callus. Double mutants combining Rg1, a mutation conferring elevated shoot formation capacity, with either dgt or Nr produced explants that formed shoots with little callus proliferation. The presence of rol + aux genes in Rg1 hairy roots prevented shoot formation. Taken together, the results suggest that although ethylene does not affect hairy root
induction, as auxin does, it may be necessary for auxin-induced callus formation in tomato. Moreover, excess auxin prevents
shoot formation in Rg1. 相似文献
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Graças Jonathas Pereira Lima Joni Esrom Peres Lázaro Eustáquio Pereira Jamet Elisabeth Dunand Christophe Vitorello Victor Alexandre Chervin Christian 《Journal of Plant Growth Regulation》2021,40(1):116-125
Journal of Plant Growth Regulation - One irreversible consequence of acidic pH for roots is cell death. Growing evidence suggests the role of hormones and cell wall-related enzymes in response to... 相似文献
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