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1.
Karlheinz Grillitsch Pablo Tarazona Lisa Klug Tamara Wriessnegger Günther Zellnig Erich Leitner Ivo Feussner Günther Daum 《生物化学与生物物理学报:生物膜》2014
Despite similarities of cellular membranes in all eukaryotes, every compartment displays characteristic and often unique features which are important for the functions of the specific organelles. In the present study, we biochemically characterized the plasma membrane of the methylotrophic yeast Pichia pastoris with emphasis on the lipids which form the matrix of this compartment. Prerequisite for this effort was the design of a standardized and reliable isolation protocol of the plasma membrane at high purity. Analysis of isolated plasma membrane samples from P. pastoris revealed an increase of phosphatidylserine and a decrease of phosphatidylcholine compared to bulk membranes. The amount of saturated fatty acids in the plasma membrane was higher than in total cell extracts. Ergosterol, the final product of the yeast sterol biosynthetic pathway, was found to be enriched in plasma membrane fractions, although markedly lower than in Saccharomyces cerevisiae. A further characteristic feature of the plasma membrane from P. pastoris was the enrichment of inositol phosphorylceramides over neutral sphingolipids, which accumulated in internal membranes. The detailed analysis of the P. pastoris plasma membrane is discussed in the light of cell biological features of this microorganism especially as a microbial cell factory for heterologous protein production. 相似文献
2.
3.
Nina Safar-Hermann Mohammed N. Ismail Han Sun Choi Erich Mstl Elmar Bamberg 《Zoo biology》1987,6(2):189-193
Estrogen concentration in feces was investigated in five different herbivorous species of zoo animals. Using a nonspecific estrogen radioimmunoassay, in four species (red buffalo, yak, Grevy's zebra, and Nubian ibex) pregnancy was revealed by measuring estrogen concentration in feces. In hippopotamus, the levels of fecal estrogens were not different between pregnant and nonpregnant animals. 相似文献
4.
Acetyl-cholinesterase and fluoride-resistant acid phosphatase activities in dorsal root ganglia in the rat 总被引:1,自引:0,他引:1
J A Vega C Rodriguez M Medina A Martinez Telleria M E Bengoechea A Perez-Casas 《Cellular and molecular biology, including cyto-enzymology》1989,35(1):39-46
Acetylcholinesterase and fluoride-resistant acid phosphatase activities were contrasted in alternative serial sections of rat dorsal root ganglia. The morphometric analysis demonstrated no correlation between cellular size and enzymatic activity. Differences with previous works in this area are discussed. 相似文献
5.
Conjugative transfer functions of broad-host-range plasmid RK2 are coregulated with vegetative replication 总被引:24,自引:0,他引:24
Mostafa Motallebi-Veshareh † Dietmar Balzer Erich Lanka Grazyna Jagura-Burdzy Christopher M. Thomas 《Molecular microbiology》1992,6(7):907-920
The kilB locus (which is unclonable in the absence of korB) of broad-host-range plasmid RK2 (60 kb) lies between the trfA operon (co-ordinates 16.4 to 18.2 kb), which encodes a protein essential for vegetative replication, and the Tra2 block of conjugative transfer genes (co-ordinates 20.0 to 27.0 kb). Promoter probe studies indicated that kilB is transcribed clockwise from a region containing closely spaced divergent promoters, one of which is the trfA promoter. The repression of both promoters by korB suggested that kilB may also play a role in stable maintenance of RK2. We have sequenced the region containing kilB and analysed it by deletion and insertion mutagenesis. Loss of the KilB+ phenotype does not result in decreased stability of mini RK2 plasmids. However insertion in ORFI (kilBI) of the region analysed results in a Tra- phenotype in plasmids which are otherwise competent for transfer, demonstrating that this locus is essential for transfer and is probably the first gene of the Tra2 region. From the kilBI DNA sequence KilBI is predicted to be 34995 Da, in line with M(r) = 36,000 observed by sodium dodecyl sulphate/polyacrylamide gel electrophoresis, and contains a type I ATP-binding motif. The purified product was used to raise antibody which allowed the level of KilBI produced from RK2 to be estimated at approximately 2000 molecules per bacterium. Protein sequence comparisons showed the highest homology score with VirB11, which is essential for the transfer of the Agrobacterium tumefaciens Ti plasmid DNA from bacteria to plant cells. The sequence similarity of both KilBI and VirB11 to a family of protein export functions suggested that KilBI may be involved in assembly of the surface-associated Tra functions. The data presented in this paper provide the first demonstration of coregulation of genes required for vegetative replication and conjugative transfer on a bacterial plasmid. 相似文献
6.
Dr. Erich Kessler 《Plant Systematics and Evolution》1985,151(1-2):67-71
The upper limit of temperature for growth is a species-specific character in the genusChlorella. The limits of 14Chlorella species range from 26–30°C (C. saccharophila) to 38–42°C (C. sorokiniana), withC. fusca var.vacuolata (34°C) andC. kessleri (34–36°C) assuming an intermediate position. Thus, there is no wide gap in the temperature limits between the normal (“low-temperature”)
species ofChlorella and the “high-temperature” species,C. sorokiniana. 相似文献
7.
Dr. Erich Buchner Sigrid Buchner Garrett Crawford William T. Mason Paul M. Salvaterra David B. Sattelle 《Cell and tissue research》1986,246(1):57-62
Summary Using a monoclonal antibody selective for the acetylcholine (ACh)-synthesizing enzyme choline acetyltransferase (ChAT) of Drosophila melanogaster we find ChAT-like immunoreactivity in specific synaptic regions throughout the brain of Drosophila melanogaster apart from the lobes and the peduncle of the mushroom body and most of the first visual neuropile (lamina). Several anatomically well-defined central brain structures exhibit particularly strong binding. Characteristic differential staining patterns are observed for each of the four neuromeres of the optic lobes. Cell bodies appear not to bind this antibody. The prominent features of the distribution of ChAT-like immunoreactivity are paralleled by the distribution of acetylcholine hydrolyzing enzymatic activity as revealed by histochemical staining for acetylcholine esterase (AChE). These results are discussed in comparison with published data on enzyme distribution, choline uptake and ACh receptor binding in the nervous system of Drosophila melanogaster. 相似文献
8.
The activities of neutral, magnesium-stimulated, and acid sphingomyelinases were measured in five regions of rat brain. Neutral enzyme activity was 2-3-fold higher in striatum than in parietal cortex and 13-fold higher than in cerebral white matter. Acid sphingomyelinase activity was more evenly distributed throughout these regions. Striatal neutral sphingomyelinase activity was not affected by treatment of rats with reserpine or haloperidol and was reduced (16%) by 6-hydroxydopamine. Striatal acid sphingomyelinase was unaffected by reserpine and 6-hydroxydopamine, and was increased (17%) by haloperidol. We conclude that neutral, magnesium-stimulated sphingomyelinase activity differs in various regions of rat brain and is particularly enriched in the corpus striatum. However, it appears to be a constitutive component of tissue rather than a readily modulated regulatory element of the catecholaminergic system. 相似文献
9.
- 1.
- 1. The net uptake of α-aminoisobutyric acid (AIB) in Ehrlich ascites tumor cells has been studied under a variety of transmembrane concentration gradients of Na+, K+ and AIB itself. 相似文献
10.
Gerhard H. Schmidt Erich Gürsch 《Ethology : formerly Zeitschrift fur Tierpsychologie》1971,28(1):19-32
Die während des Einspinnvorganges ablaufenden Verhaltensweisen werden bei Formica pratensis Retz. genau untersucht und mit anderen Ameisenarten verglichen.
- 1 Die Larve führt vor der Sekretabgabe Suchbewegungen aus, wodurch die Pflegerinnen veranlaßt werden, sie in vorher zusammengetragenes, feines Nestmaterial zu legen. Hier läßt sie eine Mulde entstehen, die ihr die Orientierung beim Kokonbau erleichtert.
- 2 Zum Kokonbau ist Nestmaterial unbedingt erforderlich; Licht als nestfremder Faktor verhindert die Gespinstanfertigung nicht.
- 3 Beim Kokonbau lassen sich drei Phasen unterscheiden: a) Anlage des Gerüstes durch Verbindung einzelner Nestpartikel zu einem strumpfförmigen Gebilde; b) Anfertigung des Außenkokons durch Abdichten der im Gerüst vorhandenen Poren; c) Bau des Innenkokons durch Spinnen von Achtertouren, wobei zunächst ein Kokonpol mit der dazugehörigen Kokonhälfte angefertigt wird und erst nach Drehung urn die Querachse der andere Pol.
- 4 Drehbewegungen um Quer- und Längsachse kommen in alien Phasen des Kokonbaues vor.
- 5 Die Achtertour wird regelmäßig durch eine Absteifbewegung, die der Formung des Kokons dient, unterbrochen.
- 6 Das plastische Verhalten der Larven beim Kokonbau wird aufgezeigt. Die Verhaltensweisen werden anhand eines Reiz-Wirkungsschemas diskutiert.