全文获取类型
收费全文 | 55243篇 |
免费 | 4682篇 |
国内免费 | 30篇 |
专业分类
59955篇 |
出版年
2023年 | 330篇 |
2022年 | 416篇 |
2021年 | 882篇 |
2020年 | 616篇 |
2019年 | 727篇 |
2018年 | 1031篇 |
2017年 | 898篇 |
2016年 | 1749篇 |
2015年 | 2919篇 |
2014年 | 3039篇 |
2013年 | 3810篇 |
2012年 | 4912篇 |
2011年 | 4312篇 |
2010年 | 2658篇 |
2009年 | 2125篇 |
2008年 | 3496篇 |
2007年 | 3386篇 |
2006年 | 3289篇 |
2005年 | 3032篇 |
2004年 | 2944篇 |
2003年 | 2714篇 |
2002年 | 2559篇 |
2001年 | 866篇 |
2000年 | 891篇 |
1999年 | 646篇 |
1998年 | 540篇 |
1997年 | 322篇 |
1996年 | 322篇 |
1995年 | 329篇 |
1994年 | 272篇 |
1993年 | 263篇 |
1992年 | 253篇 |
1991年 | 229篇 |
1990年 | 203篇 |
1989年 | 177篇 |
1988年 | 139篇 |
1987年 | 135篇 |
1986年 | 119篇 |
1985年 | 165篇 |
1984年 | 190篇 |
1983年 | 139篇 |
1982年 | 176篇 |
1981年 | 140篇 |
1980年 | 144篇 |
1979年 | 99篇 |
1978年 | 122篇 |
1977年 | 104篇 |
1976年 | 115篇 |
1975年 | 97篇 |
1974年 | 81篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Juan Viruel Pilar Catalán José Gabriel Segarra‐Moragues 《Journal of Biogeography》2012,39(6):1073-1085
Aim The Chilean endemic Dioscorea biloba (Dioscoreaceae) is a dioecious geophyte that shows a remarkable 600 km north–south disjunction in the peripheral arid area of the Atacama Desert. Its restricted present‐day distribution and probable Neogene origin indicate that its populations have a history linked to that of the Atacama Desert, making this an ideal model species with which to investigate the biogeography of the region. Location Chile, Atacama Desert and peripheral arid area. Methods Two hundred and seventy‐five individuals from nine populations were genotyped for seven nuclear microsatellite loci, and plastid trnL–F and trnT–L sequences were obtained for a representative subset of these. Analyses included the estimation of genetic diversity and population structure through clustering, Bayesian and analysis of molecular variance analyses, and statistical parsimony networks of chloroplast haplotypes. Isolation by distance was tested against alternative dispersal hypotheses. Results Microsatellite markers revealed moderate to high levels of genetic diversity within populations, with those from the southern Limarí Valley showing the highest values and northern populations showing less exclusive alleles. Bayesian analysis of microsatellite data identified three genetic groups that corresponded to geographical ranges. Chloroplast phylogeography revealed no haplotypes shared between northern and southern ranges, and little haplotype sharing between the two neighbouring southern valleys. Dispersal models suggested the presence of extinct hypothetical populations between the southern and northern ranges. Main conclusions Our results are consistent with prolonged isolation of the northern and southern groups, mediated by the life‐history traits of the species. Significant isolation was revealed at both large and moderate distances as gene flow was not evident even between neighbouring valleys. Bayesian analyses of microsatellite and chloroplast haplotype diversity identified the southern area of Limarí as the probable area of origin of the species. Our data do not support recent dispersal of D. biloba from the southern range into Antofagasta, but indicate the fragmentation of an earlier wider range, concomitant with the Pliocene–Pleistocene climatic oscillations, with subsequent extinctions of the Atacama Desert populations and the divergence of the peripheral ones as a consequence of genetic drift. 相似文献
2.
3.
Four new species ofCayaponia are described and illustrated: three from Brazil (C. cogniauxiana, C. nitida andC. rugosa) and one from Brazil and Bolivia (C. ferruginea). 相似文献
4.
5.
6.
7.
8.
Sergio Cardoso Laura Valverde Miguel A. Alfonso-Sánchez Leire Palencia-Madrid Xabier Elcoroaristizabal Jaime Algorta Susana Catarino David Arteta Rene J. Herrera María Teresa Zarrabeitia José A. Pe?a Marian M. de Pancorbo 《PloS one》2013,8(7)
The European genetic landscape has been shaped by several human migrations occurred since Paleolithic times. The accumulation of archaeological records and the concordance of different lines of genetic evidence during the last two decades have triggered an interesting debate concerning the role of ancient settlers from the Franco-Cantabrian region in the postglacial resettlement of Europe. Among the Franco-Cantabrian populations, Basques are regarded as one of the oldest and more intriguing human groups of Europe. Recent data on complete mitochondrial DNA genomes focused on macrohaplogroup R0 revealed that Basques harbor some autochthonous lineages, suggesting a genetic continuity since pre-Neolithic times. However, excluding haplogroup H, the most representative lineage of macrohaplogroup R0, the majority of maternal lineages of this area remains virtually unexplored, so that further refinement of the mtDNA phylogeny based on analyses at the highest level of resolution is crucial for a better understanding of the European prehistory. We thus explored the maternal ancestry of 548 autochthonous individuals from various Franco-Cantabrian populations and sequenced 76 mitogenomes of the most representative lineages. Interestingly, we identified three mtDNA haplogroups, U5b1f, J1c5c1 and V22, that proved to be representative of Franco-Cantabria, notably of the Basque population. The seclusion and diversity of these female genetic lineages support a local origin in the Franco-Cantabrian area during the Mesolithic of southwestern Europe, ∼10,000 years before present (YBP), with signals of expansions at ∼3,500 YBP. These findings provide robust evidence of a partial genetic continuity between contemporary autochthonous populations from the Franco-Cantabrian region, specifically the Basques, and Paleolithic/Mesolithic hunter-gatherer groups. Furthermore, our results raise the current proportion (≈15%) of the Franco-Cantabrian maternal gene pool with a putative pre-Neolithic origin to ≈35%, further supporting the notion of a predominant Paleolithic genetic substrate in extant European populations. 相似文献
9.
David M. Anderson Richard H. Scheller James W. Posakony Linda B. McAllister Steven G. Trabert Clifford Beall Roy J. Britten Eric H. Davidson 《Journal of molecular biology》1981,145(1):5-28
Three repetitive sequence families from the sea urchin genome were studied, each defined by homology with a specific cloned probe one to a few hundred nucleotides long. Recombinant λ-sea urchin DNA libraries were screened with these probes, and individual recombinants were selected that include genomic members of these families. Restriction mapping, gel blot, and kinetic analyses were carried out to determine the organization of each repeat family. Sequence elements belonging to the first of the three repeat families were found to be embedded in longer repeat sequences. These repeat sequences frequently occur in small clusters. Members of the second repeat family are also found in a long repetitive sequence environment, but these repeats usually occur singly in any given region of the DNA. The sequences of the third repeat are only 200 to 300 nucleotides long, and are generally terminated by single copy DNA, though a few examples were found associated with other repeats. These three repeat sequence families constitute sets of homologous sequence elements that relate distant regions of the DNA. 相似文献
10.