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1.
Olga N. Kulaeva Anastasiya B. Fedina Emiliya A. Burkhanova Natalya N. Karavaiko Marat Ya. Karpeisky Igor B. Kaplan Michael E. Taliansky Joseph G. Atabekov 《Plant molecular biology》1992,20(3):383-393
Exogenous human interferon 2 (IFN) and 2–5 oligoadenylates (2–5A) have been shown to cause at least a dual physiological effect in tobacco and wheat: (i) increased cytokinin activity and (ii) induced synthesis of numerous proteins, among which members of two groups of stress proteins have been identified, namely pathogenesis-related (PR) and heat shock (HS) proteins. These effects were observed only by low concentrations of these substances: IFN at 0.1–1 u/ml and 2–5A at 1–10 nM. 相似文献
2.
Nir Eynon Emiliya S. Nasibulina Lauren K. Banting Pawel Cieszczyk Agnieszka Maciejewska-Karlowska Marek Sawczuk Elvira A. Bondareva Roza R. Shagimardanova Maytal Raz Yael Sharon Alun G. Williams Ildus I. Ahmetov Alejandro Lucia Ruth Birk 《PloS one》2013,8(4)
Objective
The FTO A/T polymorphism (rs9939609) is a strong candidate to influence obesity-related traits. Elite athletes from many different sporting disciplines are characterized by low body fat. Therefore, the aim of this study was to assess whether athletic status is associated with the FTO A/T polymorphism.Subjects and Methods
A large cohort of European Caucasians from Poland, Russia and Spain were tested to examine the association between FTO A/T polymorphism (rs9939609) and athletic status. A total of 551 athletes were divided by type of sport (endurance athletes, n = 266 vs. sprint/power athletes, n = 285) as well as by level of competition (elite-level vs. national-level). The control group consisted of 1,416 ethnically-matched, non-athletic participants, all Europeans. Multinomial logistic regression analyses were conducted to assess the association between FTO A/T genotypes and athletic status/competition level.Results
There were no significantly greater/lesser odds of harbouring any type of genotype when comparing across athletic status (endurance athletes, sprint/power athletes or control participants). These effects were observed after controlling for sex and nationality. Furthermore, no significantly greater/lesser odds ratios were observed for any of the genotypes in respect to the level of competition (elite-level vs. national-level).Conclusion
The FTO A/T polymorphism is not associated with elite athletic status in the largest group of elite athletes studied to date. Large collaborations and data sharing between researchers, as presented here, are strongly recommended to enhance the research in the field of exercise genomics. 相似文献3.
Svetlana V. Kamzolova Natalia G. Vinokurova Emiliya G. Dedyukhina Vladimir A. Samoilenko Julia N. Lunina Alexey A. Mironov Ramil K. Allayarov Igor G. Morgunov 《Applied microbiology and biotechnology》2014,98(9):4149-4157
The process of succinic acid (SA) production represents the combination of microbial synthesis of α-ketoglutaric acid from rapeseed oil by yeast Yarrowia lipolytica VKM Y-2412 and subsequent decarboxylation of α-ketoglutaric acid by hydrogen peroxide to SA that leads to the production of 69.0 g l?1 of SA and 1.36 g l?1 of acetic acid. SA was isolated from the culture broth filtrate in a crystalline form. The SA recovery from the culture filtrate has certain difficulties due to the presence of residual triglycerides of rapeseed oil. The effect of different methods of the culture filtrate treatment and various sorption materials on the coagulation of triglycerides was studied, and as a result, the precipitation of residual triglycerides by acetone was chosen. The subsequent isolation procedures involved the decomposition of H2O2 in the filtrate followed by filtrate bleaching and acidification with a mineral acid, evaporation of filtrate, and SA extraction with ethanol from the residue. The purity of crystalline SA isolated from the culture broth filtrate achieved 97.6–100 %. The product yield varied from 62.6 to 71.6 % depending on the acidity of the supernatant. 相似文献
4.
Andrija Smelcerovic Predrag Dzodic Voja Pavlovic Emiliya Cherneva Denitsa Yancheva 《Amino acids》2014,46(4):825-840
Among the large family of cyclodepsipeptides, the simplest members are the cyclodidepsipeptides which have an ester group and an amide group in the same six-membered ring. To point out the pharmacological potential of this class of compounds, the present article reviews structure, isolation, synthesis and biological properties of the known cyclodidepsipeptides. Synthesis of cyclodidepsipeptides is achieved by two general approaches—by initial formation of the amide bond, or initial formation of the ester bond; and subsequent intermolecular cyclization to cyclodidepsipeptide structure. It is closely related to the condensation and ring-closure strategies applied in the preparation of the larger members of the cyclodepsipeptide family. However, due to synthesis of the smaller heretocycles it allows for the use of more versatile building blocks. There are data on antimicrobial, antioxidant and immunomodulatory activities of cyclodidepsipeptides as well as their inhibitory activities toward α-glucosidase, acyl-CoA:cholesterol acyltransferase, xanthine oxidase and platelet aggregation. Because we have recently found that two 6-(propan-2-yl)-4-methyl-morpholine-2,5-diones, as novel non-purine xanthine oxidase inhibitors, may give promise to be used in the treatment of gout, in this review we have included a study of molecular interactions of the selected cyclodidepsipeptides with xanthine oxidase using idTarget web server. Cyclodidepsipeptides showed promising pharmacological activities and meet all criteria for good solubility and permeability. However, further research of their medical application is necessary. In addition to this, the diversity of natural cyclodidepsipeptides, simplicity for synthesis and convenience for rational drug design indicate the cyclodidepsipeptide as promising scaffold in medicinal chemistry. 相似文献
5.
Diatom-inferred trophic history of IJsselmeer (The Netherlands) 总被引:1,自引:0,他引:1
Holger Cremer Frans P. M. Bunnik Emiliya P. Kirilova Eddy H. R. R. Lammens André F. Lotter 《Hydrobiologia》2009,631(1):279-287
IJsselmeer was formed in 1932 through the closure of the Afsluitdijk that separated the artificial lake from the former Zuiderzee
estuary. The palaeoecology of IJsselmeer was studied on a 63-cm-long sediment core. Lithology and microfossil data, particularly
the diatom flora, clearly show the transition from the marine Zuiderzee into the freshwater IJsselmeer. Trophic conditions
in IJsselmeer since 1932 have been inferred by qualitative and quantitative diatom-based approaches: by plotting the distribution
of trophic categories based on published trophic indicator values, by a canonical correspondence analysis (CCA) yielding relative
total phosphorus (TP) changes and by applying a transfer function in order to calculate TP concentrations. All three approaches
indicate that IJsselmeer initially was meso- to eutrophic. A first hypertrophic period is indicated for the mid-1940s, likely
due to internal loading. After 1960, the phosphorus load steadily increased and TP in IJsselmeer reached highest concentrations
(ca. 150 μg l−1) in the 1980s as confirmed by monitoring data since 1975. The monitored data show that the TP concentration continuously
decreased after 1985 due to successful environmental protection measures. This trend is not (or not yet) evidenced by the
diatom data and thus, the diatom-inferred TP concentration.
Guest editors: K. Buczkó, J. Korponai, J. Padisák & S. W. Starratt
Palaeolimnological Proxies as Tools of Environmental Reconstruction in Fresh Water 相似文献
6.
Negrin Negrev Yuri Nyagolov Margarita Stefanova Emiliya Stancheva 《Central European Journal of Biology》2011,6(4):518-523
Effects of the hormones of the hypothalamic-pituitary-thyroid axis on some basic parameters of the activity of protein C anticoagulation
pathway in rats are studied. Thyrotropin-releasing hormone (0.06 mg/kg body mass), thyrotropin (1 IU/kg), triiodothyronine
(T3) (0.08 mg/kg), thyroxine (T4) (0.08 mg/kg), administered subcutaneously for three consecutive days on four different groups
of rats increased significantly activated protein C, free protein S and protein S activity, and reduced the soluble endothelial
protein C receptor. Protein C antigen and total protein S were significantly elevated only by thyrotropin-releasing hormone
and thyroid-stimulating hormone, but they were not affected by T3 and T4 treatment. The data indicate the hypothalamic-pituitary-thyroid
axis is involved in the regulation of the protein C anticoagulation pathway in rats by activation of this system, suggesting
a tendency of hypocoagulability. 相似文献
7.
8.
Erwin Cabrera Paul Mathews Emiliya Mezhericher Thomas G. Beach Jingjing Deng Thomas A. Neubert Agueda Rostagno Jorge Ghiso 《生物化学与生物物理学报:疾病的分子基础》2018,1864(1):208-225
Extensive parenchymal and vascular Aβ deposits are pathological hallmarks of Alzheimer's disease (AD). Besides classic full-length peptides, biochemical analyses of brain deposits have revealed high degree of Aβ heterogeneity likely resulting from the action of multiple proteolytic enzymes. In spite of the numerous studies focusing in Aβ, the relevance of N- and C-terminal truncated species for AD pathogenesis remains largely understudied. In the present work, using novel antibodies specifically recognizing Aβ species N-terminally truncated at position 4 or C-terminally truncated at position 34, we provide a clear assessment of the differential topographic localization of these species in AD brains and transgenic models. Based on their distinct solubility, brain N- and C-terminal truncated species were extracted by differential fractionation and identified via immunoprecipitation coupled to mass spectrometry analysis. Biochemical/biophysical studies with synthetic homologues further confirmed the different solubility properties and contrasting fibrillogenic characteristics of the truncated species composing the brain Aβ peptidome. Aβ C-terminal degradation leads to the production of more soluble fragments likely to be more easily eliminated from the brain. On the contrary, N-terminal truncation at position 4 favors the formation of poorly soluble, aggregation prone peptides with high amyloidogenic propensity and the potential to exacerbate the fibrillar deposits, self-perpetuating the amyloidogenic loop. Detailed assessment of the molecular diversity of Aβ species composing interstitial fluid and amyloid deposits at different disease stages, as well as the evaluation of the truncation profile during various pharmacologic approaches will provide a comprehensive understanding of the still undefined contribution of Aβ truncations to the disease pathogenesis and their potential as novel therapeutic targets. 相似文献
9.
Igor G. Minkevich Emiliya G. Dedyukhina Tat’yana I. Chistyakova 《Applied microbiology and biotechnology》2010,88(3):799-806
Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was
found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to
values which can be considered as biological constants. The energy content of dry biomass strongly depended on the total lipid
content. When the lipid content was 64%, the energy value of dry biomass reached 73% of diesel oil; therefore, oleaginous
microorganisms can be a promising source of biodiesel fuel. The approach used in this work makes it possible to determine
the energy value of biomass by its elemental composition without application of laborious and expensive calorimetric measurements
of combustion heats. 相似文献
10.
Pisareva E Savov V Kujumdzieva A 《Zeitschrift für Naturforschung. C, Journal of biosciences》2005,60(1-2):116-120
Citrinin is a mycotoxin, which is produced by fungi belonging to the genus Monascus, known in biotechnology as producers of azaphilone pigments. The relation between biosynthesis of these secondary metabolites was investigated in different species of the genus Monascus in batch-culture at the following cultivation conditions: T = 28 degrees C, agitation 220 rpm, and a medium, which induce citrinin production, containing ethanol as a carbon source. The screening was carried out with 16 fungal strains and the biosynthesis of citrinin and pigments was monitored quantitatively at the standard conditions mentioned above. Some kinetic parameters of the process have been determined. The values of the growth yield coefficient Y(X/C) were between 0.32 and 0.57. The amount of the extracellular red and orange pigments at the end of cultivation varied for the different strains between 0.09 and 1.33 OU/ mg dry weight, and 0.15 and 0.96 OU/mg dry weight, respectively. The amount of the total pigments measured was between 0.16 and 3.6 OU/mg dry weight, and between 0.21 and 3.39 OU/mg dry weight. The determined ratio 500 nm/400 nm, characterizing the pigment production, ranged between 0.60 and 1.06. Twelve of the investigated strains produced citrinin and pigments, two of them produced only pigments. Two strains were not able to produce neither pigments nor citrinin. Thus, the biosynthesis of citrinin appeared to be strain-specific and does not correlate with the pigments' biosynthesis by the fungal strains belonging to the genus Monascus. 相似文献